Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection
Yıl: 2022 Cilt: 28 Sayı: 4 Sayfa Aralığı: 431 - 436 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.26927 İndeks Tarihi: 13-09-2022
Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection
Öz: Rapid milk tests for the indirect diagnosis of brucellosis are limited and generally not preferred due to insufficient diagnostic performances and inappropriate handling process. In this study, a rapid immunofiltration assay known as Non-Enzymatic Immunofiltration Assay (NERIFA) was evaluated based on diagnostic performances and applicability for the detection of anti-Brucella antibodies in bovine milk samples as milk NERIFA (mNERIFA). For this purpose, wheys, that were obtained from rennin precipitated milk constituted with reference sera, were used as test material. Besides, an enzyme-linked immunosorbent assay (ELISA) was also developed for the evaluation of mNERIFA. Aft er approval, the ELISA was evaluated as important (P<0.001) by Receiver Operating Procedures (ROC) analysis based on the diagnostic index (J) score specified as the Youden index (J: 0.963) and the Area Under the Curve (AUC) value (0.996), its results compared with mNERIFA by kappa statistical analysis. ELISA’s comparison with mNERIFA based on diagnostic performances indicated that ELISA’s performance was in perfect agreement with mNERIFA (κ=0.97) and the agreement was 0.97% (0.92-0.99%) with CI 95%. In the optimization process of mNERIFA, it was found that there was no significant diff erence between the individual and pooled whey samples (P>0.05), and a pool of 5 samples can be used instead of individual testing. In this study, it was concluded that mNERIFA may be recommended as a rapid test for anti-Brucella antibody detection in bovine milk samples based on the diagnostic performances, applicability, and pooling capacity.
Anahtar Kelime: Süt İmmünofiltrasyon Testi “mNERIFA”: Anti-Brusella Antikor Tespiti İçin Alternatif Hızlı Süt Testi
Öz: Hızlı süt testleri brusellozun indirekt teşhisi amacıyla sınırlıdır ve yetersiz teşhis özellikleri ve uygun olmayan işleme süreci nedeniyle genellikle tercih edilmez. Bu çalışmada, Non-Enzimatik İmmünofiltrasyon Testi (NERIFA) olarak bilinen hızlı bir immünfiltrasyon testi, süt NERIFA olarak tanısal performanslarına ve anti-Brucella antikor tespiti yönünden sığır süt örnekleriyle değerlendirildi. Bu amaçla referans serumlardan oluşturulan rennin ile çökeltilmiş sütten elde edilen süt serumu test materyali olarak kullanıldı. Ayrıca, mNERIFA’nın değerlendirilmesi için bir enzim immünosorbent testi (ELISA) geliştirildi. ELISA’nın, Receiver Operating Procedures (ROC) analizi ile Youden endeksi olarak bildirilen tanı indeksi (J: 0.963) ile Eğri Altındaki Alan (AUC) (0.996) değerine dayalı olarak uygunluğu (P<0.001) onaylandıktan sonra, mNERIFA ile kappa istatistiksel analizi ile karşılaştırıldı. ELISA’nın tanısal performanslara dayalı olarak mNERIFA ile karşılaştırması, ELISA’nın performansının mNERIFA ile mükemmel uyum içinde olduğunu (κ=0.97) ve bu uyumun %95 güven aralığında %0.97 (%0.92-%0.99) olduğu belirlendi. mNERIFA’nın optimizasyon sürecinde, bireysel ve havuzlanmış süt serumu örnekleri arasında anlamlı bir fark olmadığı (P>0.05) ve bireysel testler yerine 5 örneklik bir havuzun kullanılabileceği belirlendi. Bu çalışmada, mNERIFA’nın, tanısal performanslarına, uygulanabilirliğine ve havuzlama kapasitesine bağlı olarak sığır sütü örneklerinde anti-Brusella antikorlarının tespiti için hızlı bir test olarak önerilebileceği sonucuna varılmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | GENÇ E, Serdar G, kılıçoğlu y, GENÇ O (2022). Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. , 431 - 436. 10.9775/kvfd.2021.26927 |
| Chicago | GENÇ EVRIM,Serdar Gülnur,kılıçoğlu yunus,GENÇ Oktay Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. (2022): 431 - 436. 10.9775/kvfd.2021.26927 |
| MLA | GENÇ EVRIM,Serdar Gülnur,kılıçoğlu yunus,GENÇ Oktay Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. , 2022, ss.431 - 436. 10.9775/kvfd.2021.26927 |
| AMA | GENÇ E,Serdar G,kılıçoğlu y,GENÇ O Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. . 2022; 431 - 436. 10.9775/kvfd.2021.26927 |
| Vancouver | GENÇ E,Serdar G,kılıçoğlu y,GENÇ O Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. . 2022; 431 - 436. 10.9775/kvfd.2021.26927 |
| IEEE | GENÇ E,Serdar G,kılıçoğlu y,GENÇ O "Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection." , ss.431 - 436, 2022. 10.9775/kvfd.2021.26927 |
| ISNAD | GENÇ, EVRIM vd. "Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection". (2022), 431-436. https://doi.org/10.9775/kvfd.2021.26927 |
| APA | GENÇ E, Serdar G, kılıçoğlu y, GENÇ O (2022). Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(4), 431 - 436. 10.9775/kvfd.2021.26927 |
| Chicago | GENÇ EVRIM,Serdar Gülnur,kılıçoğlu yunus,GENÇ Oktay Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.4 (2022): 431 - 436. 10.9775/kvfd.2021.26927 |
| MLA | GENÇ EVRIM,Serdar Gülnur,kılıçoğlu yunus,GENÇ Oktay Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.4, 2022, ss.431 - 436. 10.9775/kvfd.2021.26927 |
| AMA | GENÇ E,Serdar G,kılıçoğlu y,GENÇ O Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 431 - 436. 10.9775/kvfd.2021.26927 |
| Vancouver | GENÇ E,Serdar G,kılıçoğlu y,GENÇ O Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 431 - 436. 10.9775/kvfd.2021.26927 |
| IEEE | GENÇ E,Serdar G,kılıçoğlu y,GENÇ O "Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.431 - 436, 2022. 10.9775/kvfd.2021.26927 |
| ISNAD | GENÇ, EVRIM vd. "Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative Rapid Bovine Milk Test for Anti-Brucella Antibody Detection". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/4 (2022), 431-436. https://doi.org/10.9775/kvfd.2021.26927 |
