TY  - JOUR
TI  - Milk Non-Enzymatic Immunofiltration Assay “mNERIFA”: An Alternative  Rapid Bovine Milk Test for Anti-Brucella Antibody Detection
AB  - Rapid milk tests for the indirect diagnosis of brucellosis are limited and generally not preferred due to insufficient diagnostic  performances and inappropriate handling process. In this study, a rapid immunofiltration assay known as Non-Enzymatic Immunofiltration  Assay (NERIFA) was evaluated based on diagnostic performances and applicability for the detection of anti-Brucella antibodies in bovine  milk samples as milk NERIFA (mNERIFA). For this purpose, wheys, that were obtained from rennin precipitated milk constituted with  reference sera, were used as test material. Besides, an enzyme-linked immunosorbent assay (ELISA) was also developed for the evaluation  of mNERIFA. Aft er approval, the ELISA was evaluated as important (P<0.001) by Receiver Operating Procedures (ROC) analysis based  on the diagnostic index (J) score specified as the Youden index (J: 0.963) and the Area Under the Curve (AUC) value (0.996), its results  compared with mNERIFA by kappa statistical analysis. ELISA’s comparison with mNERIFA based on diagnostic performances indicated  that ELISA’s performance was in perfect agreement with mNERIFA (κ=0.97) and the agreement was 0.97% (0.92-0.99%) with CI 95%.  In the optimization process of mNERIFA, it was found that there was no significant diff erence between the individual and pooled whey  samples (P>0.05), and a pool of 5 samples can be used instead of individual testing. In this study, it was concluded that mNERIFA may  be recommended as a rapid test for anti-Brucella antibody detection in bovine milk samples based on the diagnostic performances,  applicability, and pooling capacity.
AU  - GENÇ, EVRIM
AU  - GENÇ, Oktay
AU  - Serdar, Gülnur
AU  - kılıçoğlu, yunus
DO  - 10.9775/kvfd.2021.26927
PY  - 2022
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 28
IS  - 4
SN  - 1300-6045
SP  - 431
EP  - 436
DB  - TRDizin
UR  - http://search/yayin/detay/1120762
ER  -