Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the  Treatment of Osteoarthritis in Dogs

Uney, Kamil; Arıcan, Mustafa; UZUNLU, ELGİN ORÇUM; PARLAK, Kurtulus; sönmez, gonca

doi:10.9775/kvfd.2022.27357

Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs

Mustafa ARICAN, (Selçuk Üniversitesi, Veteriner Fakültesi, Cerrahi Anabilim Dalı, Konya, Türkiye)

Kamil ÜNEY, (Selçuk Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Konya, Türkiye)

Kurtuluş PARLAK, (Selçuk Üniversitesi, Veteriner Fakültesi, Cerrahi Anabilim Dalı, Konya, Türkiye)

Elgin Orçum UZUNLU, (Selçuk Üniversitesi, Veteriner Fakültesi, Cerrahi Anabilim Dalı, Konya, Türkiye)

Gonca SÖNMEZ (Selçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Konya, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2022 Cilt: 28 Sayı: 4 Sayfa Aralığı: 437 - 445 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27357 İndeks Tarihi: 13-09-2022

Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs

Öz:

Th e aim of this study was to evaluate the treatment of osteoarthritis (OA) in dogs with intra-articular injection of an (autologous platelet-rich plasma (PRP), Mesenchymal stem cells (MSCs), bio-physically activated platelet-rich plasma (B-PRP/plasma-poor platelets-PPP) and their combinations and to determine the changes in the levels of synovial fl uid samples (infl ammatory mediator, proteases (MMP-2 and MMP-9) and collagenase enzymes (MMP-1, MMP-8 and MMP-13) obtained from the joints. Total of 36 diff erent breeds, sex, age and weight of dogs with osteoarthritis were used as the material. Th e groups were divided as Group I (PRP), Group II (MSCs), Group III (PRP + MSCs), Group IV (B-PRP), Group V (B-PRP + MSCs) and group VI (saline control). Only single injection was given. Metalloproteinases were measured by using the ELISA in preoperative and postoperative treatment of 0, 15th, 30th, 60th, and 90th days in synovial fl uid samples. Clinical and radiographic examinations were performed on the 0, 15th, 30th, 60th, and 90th days. Th e results obtained from the PRP + MSCs combination group were more successful. It was also noted that successful results could be obtained with PRP alone or in combination with stem cells, especially if repeated intraarticular injections are required for osteoarthritis. New studies are needed to understand the eff ectiveness of B-PRP. Only B-PRP or stem cell combination was eff ective on many enzymes, but varying results were obtained with each case.

Anahtar Kelime:

Osteoartritli Köpeklerde Otolog Trombositten Zengin Plazma, Biyo-Fiziksel Olarak Aktive Edilmiş TZP ve Kök Hücre Tedavilerinin Proteaz ve Kollajenaz Enzim Aktivitesine Etkisi

Öz:

Bu çalışmanın amacı, köpeklerdeki OA’in tedavisinde eklem içi enjeksiyonu yapılan, otolog trombositten zengin plazma (PRP), Mezenkimal kök hücreler (MSC’ler), biyo-fiziksel olarak aktive edilmiş trombositten zengin plazma (B-PRP/plazmadan fakir trombositler-PPP) ve kombinasyonlarının etkinliğinin, sinovyal sıvı örneklerinde (yangısel mediator, proteazlar (MMP-2 ve MMP-9) ve kollajenaz enzimlerinin (MMP 1, MMP-8 ve MMP-13) düzeylerindeki değişiklikleri değerlendirmektir. Farklı cins, cinsiyet, yaş ve kiloda OA teşhisi konulan 36 köpek kullanıldı. Gruplar; Grup I (PRP), Grup II (MSC’ler), Grup III (PRP + MSC’ler), Grup IV (B-PRP), Grup V (B-PRP + MSC’ler) ve Grup VI (kontrol) oluşturdu. Sadece tek intra artiküler enjeksiyon yapıldı. Operasyon öncesi ve tedavi sonrası 0, 15, 30, 60 ve 90. günlerde metalloproteinaz seviyeleri ELISA ile ölçüldü. Sinovyal sıvı örneklerinde klinik ve radyografik muayeneler 0, 15, 30, 60 ve 90. günlerde yapıldı. PRP + MSC kombinasyon grubundan elde edilen sonuçların başarılı olduğu gözlendi. Özellikle osteoartritin tedavisinde tekrarlayan intraartiküler enjeksiyonlar gerekiyorsa, PRP tek başına veya kök hücrelerle kombinasyon kullanımı halinde başarılı sonuçlar alınabileceği kaydedildi. B-PRP’nin etkinliğini anlamak için yeni çalışmalara ihtiyaç olduğu gözlendi. Genelde B-PRP veya kök hücre kombinasyonu birçok enzim üzerine etkili olduğu kayıt edidi. Ancak vakalar arasında farklı sonuçlar elde edildi.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Arıcan M, Uney K, PARLAK K, UZUNLU E, sönmez g (2022). Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. , 437 - 445. 10.9775/kvfd.2022.27357
Chicago	Arıcan Mustafa,Uney Kamil,PARLAK Kurtulus,UZUNLU ELGİN ORÇUM,sönmez gonca Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. (2022): 437 - 445. 10.9775/kvfd.2022.27357
MLA	Arıcan Mustafa,Uney Kamil,PARLAK Kurtulus,UZUNLU ELGİN ORÇUM,sönmez gonca Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. , 2022, ss.437 - 445. 10.9775/kvfd.2022.27357
AMA	Arıcan M,Uney K,PARLAK K,UZUNLU E,sönmez g Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. . 2022; 437 - 445. 10.9775/kvfd.2022.27357
Vancouver	Arıcan M,Uney K,PARLAK K,UZUNLU E,sönmez g Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. . 2022; 437 - 445. 10.9775/kvfd.2022.27357
IEEE	Arıcan M,Uney K,PARLAK K,UZUNLU E,sönmez g "Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs." , ss.437 - 445, 2022. 10.9775/kvfd.2022.27357
ISNAD	Arıcan, Mustafa vd. "Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs". (2022), 437-445. https://doi.org/10.9775/kvfd.2022.27357

APA	Arıcan M, Uney K, PARLAK K, UZUNLU E, sönmez g (2022). Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(4), 437 - 445. 10.9775/kvfd.2022.27357
Chicago	Arıcan Mustafa,Uney Kamil,PARLAK Kurtulus,UZUNLU ELGİN ORÇUM,sönmez gonca Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.4 (2022): 437 - 445. 10.9775/kvfd.2022.27357
MLA	Arıcan Mustafa,Uney Kamil,PARLAK Kurtulus,UZUNLU ELGİN ORÇUM,sönmez gonca Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.4, 2022, ss.437 - 445. 10.9775/kvfd.2022.27357
AMA	Arıcan M,Uney K,PARLAK K,UZUNLU E,sönmez g Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 437 - 445. 10.9775/kvfd.2022.27357
Vancouver	Arıcan M,Uney K,PARLAK K,UZUNLU E,sönmez g Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 437 - 445. 10.9775/kvfd.2022.27357
IEEE	Arıcan M,Uney K,PARLAK K,UZUNLU E,sönmez g "Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.437 - 445, 2022. 10.9775/kvfd.2022.27357
ISNAD	Arıcan, Mustafa vd. "Proteases and Collagenase Enzymes Activity Aft er Autologous Platelet Rich Plasma, Bio-Physically Activated PRP and Stem Cells for the Treatment of Osteoarthritis in Dogs". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/4 (2022), 437-445. https://doi.org/10.9775/kvfd.2022.27357