Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience
Yıl: 2022 Cilt: 28 Sayı: 4 Sayfa Aralığı: 455 - 460 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27457 İndeks Tarihi: 14-09-2022
Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience
Öz: The aim of this study was to investigate the eff ects of various fetal calf serum (FCS) doses on %1 soybean lecithin-based semen extenders for goat semen cryopreservation and the impacts on spermatological parameters over post-thaw and post-incubation (6 h) time periods. Sperm samples collected via electro-ejaculation method were pooled to overcome the individual diff erences and were used in the study. Th e samples then were split into four equal aliquots to create study groups as; 0.25%, 0.50%, 0.75% FCS supplemented and a sample of FCS-free control group. Each sample group was diluted to approximately 150 x 106 spermatozoon/mL final concentration and two step dilution method was used for cryopreservation. Study groups were examined for sperm motility, plasma membrane functional integrity with hypoosmotic swelling test (HOST), acrosome integrity by FITC-Pisum sativum agglutinin (PSA-FITC) and DNA damage by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL). All samples were incubated for further 6 h in a humidified air chamber with 5% CO2 at 39°C. Th e results indicated that FCS supplementation in soybean lecithin-based extenders for goat sperm cryopreservation had significant eff ects on post-thawing time point motility (P<0.05), plasma membrane integrity (P<0.05) and acrosomal integrity (P<0.05) parameters. Subsequently to 6 h of incubation period, DNA integrity results yielded better scores comparing to control group in addition with other spermatological parameters (P<0.05).
Anahtar Kelime: Lesitin Bazlı Teke Sperma Sulandırıcılarına Eklenen Fötal Buzağı Serumunun Spermanın Dondurulma ve İnkübasyon Direnci Üzerine Etkileri
Öz: Bu çalışmanın amacı teke spermasının dondurulmasında kullanılacak %1 soya lesitini bazlı sulandırıcıya eklenen çeşitli dozlarda fötal buzağı serumunun (FBS) dondurma-çözdürme ve 6 saatlik inkübasyon süreci sonrası dönemde spermatolojik parametrelere etkilerini araştırmaktır. Sperma örnekleri elektro-ejakülatör ile alınmış ve bireysel farklılıkların üstesinden gelinmesi amacıyla birleştirilmiştir (pooling). Birleştirilen örnekler dört eşit gruba bölünmüş ve %0.25, %0.50, %0.75 FBS eklenmiş ve FBS eklenmemiş kontrol olacak şekilde çalışma grupları oluşturulmuştur. Her çalışma grubu dondurulma amacıyla 150 x 106 spermatozoon/mL final konsantrasyon olacak şekilde iki aşamalı olarak sulandırılmıştır. Çalışma grupları sperma motilitesi, hipo-ozmotik şişme testi ile ölçülen plazma membran fonksiyonel bütünlüğü, FITC-Pisum sativum agglutinin (FITC-PSA) ile ölçülen akrozomal bütünlük ve terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling assay (TUNEL) testi ile ölçülen DNA bütünlüğü açısından incelenmiştir. Tüm gruplar çözdürme sonrası dönemde inkübasyon direncinin ölçülmesi amacıyla 6 saat boyunca %5 CO2 ve 39°C sıcaklıklığa ayarlanmış bir ortamda inkübe edilmiştir. Çalışmanın sonuçları soya lesitini bazlı sperma dondurma sulandırıcılarına FBS eklenmesinin çözdürme sonrası motilite (P<0.05), plazma membran bütünlüğü (P<0.05) ve akrozomal bütünlük üzerine istatistiki anlam düzeyinde fark yarattığını göstermiştir. Buna ek olarak, gerçekleştirilen 6 saatlik inkübasyon sürecinin sonucunda FBS’nin adı geçen spermatolojik parametrelere ek olarak DNA bütünlüğü sonuçlarında da pozitif etki yarattığı tespit edilmiştir (P<0.05).
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Salmani H, Nabi MM, Vaseghi-Dodaran H, Rahman MB, Mohammadi Sangcheshmeh A, Shakeri M, Towhidi A, Shahneh AZ, Zhandi M: Effect of glutathione in soybean lecithin-based semen extender on goat semen quality after freeze-thawing. Small Ruminant Res, 112, 123-127, 2013. DOI: 10.1016/j.smallrumres.2012.12.015
- 2. Aisen EG, Medina VH, Venturino A: Cryopreservation and post thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57, 1801-1808, 2002. DOI: 10.1016/S0093-691X(02)00653-2
- 3. Jiménez-Rabadán P, Ramón M, García-Álvarez O, Maroto-Morales A, Álvaro-García PJ, Del Olmo E, Pérez-Guzmán MD, Fernández-Santos MR, Julián Garde J, Soler AJ: Improved cryopreservation protocol for Blanca-Celtibérica buck semen collected by electroejaculation. Cryobiology, 67, 251-257, 2013. DOI: 10.1016/j.cryobiol.2013.08.002
- 4. Salmani H, Towhidi A, Zhandi M, Bahreini M, Sharafi M: In vitro assessment of soybean lecithin and egg yolk based diluents for cryo preservation of goat semen. Cryobiology, 68, 276-280, 2014. DOI: 10.1016/j. cryobiol.2014.02.008
- 5. Purdy PH: A review on goat sperm cryopreservation. Small Ruminant Res, 63, 215-225, 2006. DOI: 10.1016/j.smallrumres.2005.02.015
- 6. Gangwar C, Kharche SD, Kumar S, Jindal SK: Cryopreservation of goat semen: Status and prospects. Indian J Small Ruminants, 22 (1): 1-10, 2016. DOI: 10.5958/0973-9718.2016.00005.2
- 7. Mata-Campuzano M, Álvarez-Rodríguez M, Álvarez M, Tamayo Canul J, Anel L, de Paz P, Martínez-Pastor F: Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology, 83, 520-528, 2015. DOI: 10.1016/j.theriogenology.2014.10.018
- 8. Zhandi M, Sharafi M: Negative effect of combined cysteine and glutathione in soy lecithin-based extender on post-thawed ram spermatozoa. Cell Tissue Bank, 16, 443-448, 2015. DOI: 10.1007/s10561-014-9488-z
- 9. Forouzanfar M, Sharafi M, Hosseini SM, Ostadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH: In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology, 73, 480-487, 2010. DOI: 10.1016/j.theriogenology.2009.10.005
- 10. Alcay S, Toker MB, Onder NT, Gokce E: Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa. Cryobiology, 74, 81-85, 2017. DOI: 10.1016/j. cryobiol.2016.11.011
- 11. Rezaei Z, Zeynodini S, Baghshahi H, Towhidi A, Faraji S: The effect of adding pyridoxine to soybean lecithin-based extender on goat semen quality parameters after the freeze- thawing process. Iranian J Vet Res, 22 (3): 234- 238, 2021. DOI: 10.22099/ijvr.2021.39828.5777
- 12. Sun L, Fan W, Wu C, Zhang S, Dai J, Zhang D: Effect of substituting different concentrations of soybean lecithin and egg yolk in tris-based extender on goat semen cryopreservation. Cryobiology, 92, 146-150, 2020. DOI: 10.1016/j.cryobiol.2019.12.004
- 13. Ortman K, Rodriguez Martinez H: Membrane damage during dilution, cooling and freezing thawing of boar spermatozoa packaged in plastic bags. J Vet Med Ser A, 41, 37-47, 1994. DOI: 10.1111/j.1439-0442.1994.tb00063.x
- 14. Ntemka A, Tsakmakidis IA, Kiossis E, Milovanovic A, Boscos CM: Current status and advances in ram semen cryopreservation. J Hell Vet Med Soc, 69 (2): 911-924, 2018. DOI: 10.12681/jhvms.18014
- 15. Garzón DL, Peñaranda DS, Pérez L, Marco-Jiménez F, Espert X, Müller T, Jover M, Asturiano JF: Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of European eel sperm. Reprod Domest Anim, 43, 99-105, 2008. DOI: 10.1111/ j.1439-0531.2007.00861.x
- 16. Blank MH, Silva VC, Rui BR, Novaes GA, Castiglione VC, Garcia Pereira RJ: Beneficial influence of fetal bovine serum on in vitro cryosurvival of chicken spermatozoa. Cryobiology, 95, 103-109, 2020. DOI: 10.1016/j. cryobiol.2020.05.011
- 17. Sarıözkan S, Türk G, Cantürk F, Yay A, Eken A, Akçay A: The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67, 1-6, 2013. DOI: 10.1016/j.cryobiol.2013.04.002
- 18. Chen L, Mao SJT, Larsen WJ: Identification of a factor in fetal bovine serum that stabilizes the cumulus extracellular matrix: A role for a member of the inter-α-trypsin inhibitor family. J Biol Chem, 267, 12380-12386, 1992. DOI: 10.1016/S0021-9258(19)49851-7
- 19. Ustuner B, Gokce E, Toker MB, Onder NT, Soylu MK, Sagirkaya H, Nur Z: Effect of sperm pooling with seminal plasma collected in breeding or nonbreeding season on Saanen goat sperm cryosurvival. Andrologia, 50 (4):e12968, 2018. DOI: 10.1111/and.12968
- 20. Toker MB, Alcay S, Gokce E, Ustuner B: Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience. Cryobiology, 72, 205-209, 2016. DOI: 10.1016/j.cryobiol.2016.05.001
- 21. Alcay S, Aktar A, Koca D, Kilic MA, Akkasoglu M, Sagirkaya H: Positive effect of autologous platelet rich plasma on Saanen buck semen cryopreservation in non-breeding season. Cryobiology, 103, 45-48, 2021. DOI: 10.1016/j.cryobiol.2021.09.012
- 22. Alçay S, Toker B, Üstüner B, Nur Z, Sağirkaya H, Soylu MK: Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Univ Vet Fak Derg, 20 (5): 793-798, 2014. DOI: 10.9775/kvfd.2014.11144
- 23. Ustuner B, Alcay S, Toker MB, Nur Z, Gokce E, Sonat FA, Gul Z, Duman M, Ceniz C, Uslu A, Sagirkaya H, Soylu MK: Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk based extender. Anim Reprod Sci, 164, 97-104, 2016. DOI: 10.1016/j. anireprosci.2015.11.017
- 24. Hammadeh ME, Dehn C, Hippach M, Zeginiadou T, Stieber M, Georg T, Rosenbaum P, Schmidt W: Comparison between computerized slow-stage and static liquid nitrogen vapour freezing methods with respect to the deleterious effect on chromatin and morphology of spermatozoa from fertile and subfertile men. Int J Androl, 24, 66-72, 2001. DOI: 10.1046/j.1365- 2605.2001.00270.x
- 25. Alçay S, Toker MB, Gökçe E, Önder NT, Üstüner B, Nur Z: Long term incubation resilience of post-thaw ram semen diluted with lecithin-based extender supplemented with bovine serum albumin. Kafkas Univ Vet Fak Derg, 25 (3): 291-297, 2019. DOI: 10.9775/kvfd.2018.20843
- 26. Yodmingkwan P, Guntaprom S, Jaksamrit J, Lertchunhakiat K: Effects of extenders on fresh and freezing semen of boer goat. Agric Sci Procedia, 11, 125-130, 2016. DOI: 10.1016/j.aaspro.2016.12.021
- 27. Oke M, Jacob JK, Paliyath G: Effect of soy lecithin in enhancing fruit juice/sauce quality. Food Res Int, 43, 232-240, 2010. DOI: 10.1016/j. foodres.2009.09.021
- 28. Chelucci S, Pasciu V, Succu S, Addis D, Leoni GG, Manca ME, Naitana S, Berlinguer F: Soybean lecithin-based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryo preservation. Theriogenology, 83, 1064-1074, 2015. DOI: 10.1016/j. theriogenology.2014.12.012
- 29. Barcelo-Fimbres M, Seidel Jr GE: Effects of fetal calf serum, phenazine ethosulfate and either glucose or fructose during in vitro culture of bovine embryos on embryonic development after cryopreservation. Mol Reprod Dev, 74, 1395-1405, 2007. DOI: 10.1002/mrd.20699
- 30. Gstraunthaler G: Alternatives to the use of fetal bovine serum: Serum free cell culture. ALTEX, 20 (4): 275-281, 2003. DOI: 10.14573/altex. 2003.4.257
- 31. Price PJ, Gregory EA: Relationship between in vitro growth promotion and biophysical and biochemical properties of the serum supplement. In Vitro, 18, 576-584, 1982. DOI: 10.1007/BF02810081
- 32. Sandal Aİ, Şenlikci H, Yılmazer M, Kartal B, Palabıyık B, Özdaş ÖB: Determination of oxidative stress parameters and DNA fragmentation on post-thawed buck semen in the presence of ram seminal plasma and fetal calf serum. Andrologia, 53, 1-8, 2021. DOI: 10.1111/and.14032
- 33. Wang W, Luo J, Sun S, Xi L, Gao Q, Haile AB, Shi H, Zhang W, Shi H: The effect of season on spermatozoa motility, plasma membrane and acrosome integrity in fresh and frozen-thawed semen from xinong saanen bucks. Reprod Domest Anim, 50, 23-28, 2015. DOI: 10.1111/rda.12444
- 34. Memon AA, Wahid H, Rosnina Y, Goh YM, Ebrahimi M, Nadia FM, Audrey G: Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender. Anim Reprod Sci, 129, 44-49, 2011. DOI: 10.1016/j.anireprosci.2011.10.004
- 35. Sun W, Jiang S, Su J, Zhang J, Bao X, Ding R, Shi P, Li S, Wu C, Zhao G, Cao G, Sun QY, Yu H, Li X: The effects of cryopreservation on the acrosome structure, enzyme activity, motility, and fertility of bovine, ovine, and goat sperm. Anim Reprod, 17 (4): e20200219, 2020. DOI: 10.1590/1984-3143- AR2020-0219
- 36. Anakkul N, Suwimonteerabutr J, Tharasanit T, Phutikanit N, Singlor J, Techakumphu M: Glycerol concentration effects on quality and longevity of post-thaw goat semen. Thai J Vet Med, 43 (2): 179-186, 2013.
| APA | Toker M, Alçay S (2022). Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. , 455 - 460. 10.9775/kvfd.2022.27457 |
| Chicago | Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. (2022): 455 - 460. 10.9775/kvfd.2022.27457 |
| MLA | Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. , 2022, ss.455 - 460. 10.9775/kvfd.2022.27457 |
| AMA | Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. . 2022; 455 - 460. 10.9775/kvfd.2022.27457 |
| Vancouver | Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. . 2022; 455 - 460. 10.9775/kvfd.2022.27457 |
| IEEE | Toker M,Alçay S "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience." , ss.455 - 460, 2022. 10.9775/kvfd.2022.27457 |
| ISNAD | Toker, M.Berk - Alçay, Selim. "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience". (2022), 455-460. https://doi.org/10.9775/kvfd.2022.27457 |
| APA | Toker M, Alçay S (2022). Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(4), 455 - 460. 10.9775/kvfd.2022.27457 |
| Chicago | Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.4 (2022): 455 - 460. 10.9775/kvfd.2022.27457 |
| MLA | Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.4, 2022, ss.455 - 460. 10.9775/kvfd.2022.27457 |
| AMA | Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 455 - 460. 10.9775/kvfd.2022.27457 |
| Vancouver | Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 455 - 460. 10.9775/kvfd.2022.27457 |
| IEEE | Toker M,Alçay S "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.455 - 460, 2022. 10.9775/kvfd.2022.27457 |
| ISNAD | Toker, M.Berk - Alçay, Selim. "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/4 (2022), 455-460. https://doi.org/10.9775/kvfd.2022.27457 |
