Yıl: 2022 Cilt: 28 Sayı: 4 Sayfa Aralığı: 455 - 460 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27457 İndeks Tarihi: 14-09-2022

Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience

Öz:
The aim of this study was to investigate the eff ects of various fetal calf serum (FCS) doses on %1 soybean lecithin-based semen extenders for goat semen cryopreservation and the impacts on spermatological parameters over post-thaw and post-incubation (6 h) time periods. Sperm samples collected via electro-ejaculation method were pooled to overcome the individual diff erences and were used in the study. Th e samples then were split into four equal aliquots to create study groups as; 0.25%, 0.50%, 0.75% FCS supplemented and a sample of FCS-free control group. Each sample group was diluted to approximately 150 x 106 spermatozoon/mL final concentration and two step dilution method was used for cryopreservation. Study groups were examined for sperm motility, plasma membrane functional integrity with hypoosmotic swelling test (HOST), acrosome integrity by FITC-Pisum sativum agglutinin (PSA-FITC) and DNA damage by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL). All samples were incubated for further 6 h in a humidified air chamber with 5% CO2 at 39°C. Th e results indicated that FCS supplementation in soybean lecithin-based extenders for goat sperm cryopreservation had significant eff ects on post-thawing time point motility (P<0.05), plasma membrane integrity (P<0.05) and acrosomal integrity (P<0.05) parameters. Subsequently to 6 h of incubation period, DNA integrity results yielded better scores comparing to control group in addition with other spermatological parameters (P<0.05).
Anahtar Kelime:

Lesitin Bazlı Teke Sperma Sulandırıcılarına Eklenen Fötal Buzağı Serumunun Spermanın Dondurulma ve İnkübasyon Direnci Üzerine Etkileri

Öz:
Bu çalışmanın amacı teke spermasının dondurulmasında kullanılacak %1 soya lesitini bazlı sulandırıcıya eklenen çeşitli dozlarda fötal buzağı serumunun (FBS) dondurma-çözdürme ve 6 saatlik inkübasyon süreci sonrası dönemde spermatolojik parametrelere etkilerini araştırmaktır. Sperma örnekleri elektro-ejakülatör ile alınmış ve bireysel farklılıkların üstesinden gelinmesi amacıyla birleştirilmiştir (pooling). Birleştirilen örnekler dört eşit gruba bölünmüş ve %0.25, %0.50, %0.75 FBS eklenmiş ve FBS eklenmemiş kontrol olacak şekilde çalışma grupları oluşturulmuştur. Her çalışma grubu dondurulma amacıyla 150 x 106 spermatozoon/mL final konsantrasyon olacak şekilde iki aşamalı olarak sulandırılmıştır. Çalışma grupları sperma motilitesi, hipo-ozmotik şişme testi ile ölçülen plazma membran fonksiyonel bütünlüğü, FITC-Pisum sativum agglutinin (FITC-PSA) ile ölçülen akrozomal bütünlük ve terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling assay (TUNEL) testi ile ölçülen DNA bütünlüğü açısından incelenmiştir. Tüm gruplar çözdürme sonrası dönemde inkübasyon direncinin ölçülmesi amacıyla 6 saat boyunca %5 CO2 ve 39°C sıcaklıklığa ayarlanmış bir ortamda inkübe edilmiştir. Çalışmanın sonuçları soya lesitini bazlı sperma dondurma sulandırıcılarına FBS eklenmesinin çözdürme sonrası motilite (P<0.05), plazma membran bütünlüğü (P<0.05) ve akrozomal bütünlük üzerine istatistiki anlam düzeyinde fark yarattığını göstermiştir. Buna ek olarak, gerçekleştirilen 6 saatlik inkübasyon sürecinin sonucunda FBS’nin adı geçen spermatolojik parametrelere ek olarak DNA bütünlüğü sonuçlarında da pozitif etki yarattığı tespit edilmiştir (P<0.05).
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Toker M, Alçay S (2022). Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. , 455 - 460. 10.9775/kvfd.2022.27457
Chicago Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. (2022): 455 - 460. 10.9775/kvfd.2022.27457
MLA Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. , 2022, ss.455 - 460. 10.9775/kvfd.2022.27457
AMA Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. . 2022; 455 - 460. 10.9775/kvfd.2022.27457
Vancouver Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. . 2022; 455 - 460. 10.9775/kvfd.2022.27457
IEEE Toker M,Alçay S "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience." , ss.455 - 460, 2022. 10.9775/kvfd.2022.27457
ISNAD Toker, M.Berk - Alçay, Selim. "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience". (2022), 455-460. https://doi.org/10.9775/kvfd.2022.27457
APA Toker M, Alçay S (2022). Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(4), 455 - 460. 10.9775/kvfd.2022.27457
Chicago Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.4 (2022): 455 - 460. 10.9775/kvfd.2022.27457
MLA Toker M.Berk,Alçay Selim Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.4, 2022, ss.455 - 460. 10.9775/kvfd.2022.27457
AMA Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 455 - 460. 10.9775/kvfd.2022.27457
Vancouver Toker M,Alçay S Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 455 - 460. 10.9775/kvfd.2022.27457
IEEE Toker M,Alçay S "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.455 - 460, 2022. 10.9775/kvfd.2022.27457
ISNAD Toker, M.Berk - Alçay, Selim. "Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based Goat Semen Cryopreservation Extenders and Impacts on Incubation Resilience". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/4 (2022), 455-460. https://doi.org/10.9775/kvfd.2022.27457