TY  - JOUR
TI  - Comprehensive Eff ects of Fetal Calf Serum in Soybean-Lecithin Based  Goat Semen Cryopreservation Extenders and Impacts on Incubation  Resilience
AB  - The aim of this study was to investigate the eff ects of various fetal calf serum (FCS) doses on %1 soybean lecithin-based semen  extenders for goat semen cryopreservation and the impacts on spermatological parameters over post-thaw and post-incubation (6 h) time  periods. Sperm samples collected via electro-ejaculation method were pooled to overcome the individual diff erences and were used in  the study. Th e samples then were split into four equal aliquots to create study groups as; 0.25%, 0.50%, 0.75% FCS supplemented and a  sample of FCS-free control group. Each sample group was diluted to approximately 150 x 106  spermatozoon/mL final concentration and  two step dilution method was used for cryopreservation. Study groups were examined for sperm motility, plasma membrane functional  integrity with hypoosmotic swelling test (HOST), acrosome integrity by FITC-Pisum sativum agglutinin (PSA-FITC) and DNA damage by  terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL). All samples were incubated for further 6 h in a  humidified air chamber with 5% CO2 at 39°C. Th e results indicated that FCS supplementation in soybean lecithin-based extenders for goat  sperm cryopreservation had significant eff ects on post-thawing time point motility (P<0.05), plasma membrane integrity (P<0.05) and  acrosomal integrity (P<0.05) parameters. Subsequently to 6 h of incubation period, DNA integrity results yielded better scores comparing  to control group in addition with other spermatological parameters (P<0.05).
AU  - Toker, M.Berk
AU  - Alçay, Selim
DO  - 10.9775/kvfd.2022.27457
PY  - 2022
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 28
IS  - 4
SN  - 1300-6045
SP  - 455
EP  - 460
DB  - TRDizin
UR  - http://search/yayin/detay/1120816
ER  -