Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a  Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye

Karayel-Hacioglu, Ilke; Alkan, Feray; Duran-Yelken, Selda

doi:10.9775/kvfd.2022.27656

Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye

İlke KARAYEL HACIOĞLU, (Ankara Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Ankara, Türkiye)

Selda DURAN YELKEN, (Siirt Üniversitesi Veteriner Fakültesi Viroloji Anabilim Dalı, Siirt, Türkiye)

Feray ALKAN (Ankara Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Ankara, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2022 Cilt: 28 Sayı: 4 Sayfa Aralığı: 499 - 506 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27656 İndeks Tarihi: 14-09-2022

Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye

Öz:

Enteric diseases are considered an important factor aff ecting the livestock industry; however, there are limited data on viruses that cause enteric diseases in small ruminants in our country. In this study, we focused on enterovirus (EV), hunnivirus (HuV), and kobuvirus (KoV), which are members of the family of Picornaviridae. In order to provide further information on the prevalence and the molecular epidemiology of these viruses, a total of 73 fecal samples or gut contents from diarrheic sheep and goats were screened for EV, HuV, and KoV. For this purpose, RT-PCRs were performed by using the specific primers for each virus. Th e prevalence rate determined in the sampled population was 2.7% (2/73) for each virus. In detail, EVs (2/60, 3.3%) and HuVs (2/60, 3.3%) were each detected in only sheep samples while KoVs were identified in a goat sample (1/13, 7.6%) and a sheep sample (1/60, 1.6%). Th ere was no evidence of coinfection with these viruses in the tested animals. According to the results of the molecular analyzes, our EVs were clustered in caprine/ovine specific EV-G and HuV strains retrieved in this study were grouped along with the other caprine/ovine origin sequences in Hunnivirus A2 genotype. Moreover, it was observed that the detected KoVs clustered in distinct species: Aichivirus B and Aichivirus C. In conclusion, this study, which reported the detection of EVs and HuVs from sheep as well as KoVs from sheep and goats in our country, provides valuable data on the epidemiology and molecular characteristics of these viruses.

Anahtar Kelime:

Bir Bakışta İshalli Küçük Ruminantlarda Picornavirusların Moleküler Tespiti: Türkiye’de Enterovirus, Hunnivirus ve Kobuvirus

Öz:

Enterik hastalıklar, hayvancılık endüstrisini etkileyen önemli bir faktör olarak kabul edilmektedir, ancak ülkemizde küçük ruminantlarda enterik hastalıklara neden olan viruslara ilişkin veriler sınırlıdır. Bu çalışmada Picornaviridae ailesinin üyeleri olan enterovirus (EV), hunnivirus (HuV) ve kobuvirus (KoV) üzerinde durulmuştur. Bu virusların prevalansı ve moleküler epidemiyolojisi hakkında daha fazla bilgi elde etmek amacıyla, ishalli koyun ve keçilerden toplam 73 dışkı örneği veya bağırsak içeriği EV, HuV ve KoV için test edilmiştir. Bu amaçla, her bir virus için spesifik primerler kullanılarak RT-PCR’lar yapılmıştır. Örneklenen popülasyonda tespit edilen prevalans oranı her bir virus için %2.7 (2/73) olarak hesaplanmıştır. Detaylı olarak değerlendirildiğinde, EV (2/60, %3.3) ve HuV (2/60, %3.3) yalnızca koyun örneklerinde saptanırken, KoV ise bir keçi (1/13, %7.6) ve bir koyun örneğinde (1/60, %1.6) tespit edilmiştir. Bununla birlikte, test edilen hayvanlarda bu viruslarla herhangi bir koenfeksiyon saptanmamıştır. Moleküler analizlerin sonuçlarına göre, EV suşlarının keçi/koyuna özgü EV-G’de kümelendiği ve bu çalışmada elde edilen HuV suşlarının ise Hunnivirus A2 genotipinde diğer keçi/koyun kökenli dizinlerle birlikte gruplandığı ortaya konulmuştur. Ayrıca tespit edilen KoV suşlarının Aichivirus B ve Aichivirus C olmak üzere farklı türlerde kümelendiği gözlemlenmiştir. Sonuç olarak, ülkemizde koyun ve keçilerde KoV’ların yanı sıra koyunlarda EV ve HuV’un tespitini bildiren bu çalışma, bu virusların epidemiyolojisi ve moleküler özelliklerine ilişkin değerli veriler sunmaktadır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Karayel-Hacioglu I, Duran-Yelken S, Alkan F (2022). Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. , 499 - 506. 10.9775/kvfd.2022.27656
Chicago	Karayel-Hacioglu Ilke,Duran-Yelken Selda,Alkan Feray Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. (2022): 499 - 506. 10.9775/kvfd.2022.27656
MLA	Karayel-Hacioglu Ilke,Duran-Yelken Selda,Alkan Feray Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. , 2022, ss.499 - 506. 10.9775/kvfd.2022.27656
AMA	Karayel-Hacioglu I,Duran-Yelken S,Alkan F Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. . 2022; 499 - 506. 10.9775/kvfd.2022.27656
Vancouver	Karayel-Hacioglu I,Duran-Yelken S,Alkan F Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. . 2022; 499 - 506. 10.9775/kvfd.2022.27656
IEEE	Karayel-Hacioglu I,Duran-Yelken S,Alkan F "Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye." , ss.499 - 506, 2022. 10.9775/kvfd.2022.27656
ISNAD	Karayel-Hacioglu, Ilke vd. "Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye". (2022), 499-506. https://doi.org/10.9775/kvfd.2022.27656

APA	Karayel-Hacioglu I, Duran-Yelken S, Alkan F (2022). Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(4), 499 - 506. 10.9775/kvfd.2022.27656
Chicago	Karayel-Hacioglu Ilke,Duran-Yelken Selda,Alkan Feray Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.4 (2022): 499 - 506. 10.9775/kvfd.2022.27656
MLA	Karayel-Hacioglu Ilke,Duran-Yelken Selda,Alkan Feray Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.4, 2022, ss.499 - 506. 10.9775/kvfd.2022.27656
AMA	Karayel-Hacioglu I,Duran-Yelken S,Alkan F Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 499 - 506. 10.9775/kvfd.2022.27656
Vancouver	Karayel-Hacioglu I,Duran-Yelken S,Alkan F Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(4): 499 - 506. 10.9775/kvfd.2022.27656
IEEE	Karayel-Hacioglu I,Duran-Yelken S,Alkan F "Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.499 - 506, 2022. 10.9775/kvfd.2022.27656
ISNAD	Karayel-Hacioglu, Ilke vd. "Molecular Detection of Picornaviruses in Diarrheic Small Ruminants at a Glance: Enterovirus, Hunnivirus, and Kobuvirus in Türkiye". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/4 (2022), 499-506. https://doi.org/10.9775/kvfd.2022.27656