Objective: This study was planned to examine the relationship between nurses' education level, night shift work, and vital signs follow-up. Methods: The population of the study, which was planned as a descriptive and cross-sectional study, consisted of 389 nurses working in a training and research hospital in Istanbul between May 2020-January 2021; the sample consisted of 241 nurses. Data were collected with Nurse Information Form and Health Professionals' Opinion Form on Monitoring Nocturnal Vital Findings. Results: It was determined that 77.6% of the nurses were female, mean age was 26.10±4.86 years, and 61.8% were undergraduate graduates. In the follow-up of vital signs, it was determined that 40.2% of the nurses used monitors, 35.7% of them used a manual device, 26% of them used both monitors and manual tools. For the monitoring of night vital signs of nurses; it was determined that the mean scores of Factor 1 (Workload, Resources and Capacity) Factor 2 (Prioritization), Factor 3 (Safety Culture), and Factor 4 (Responsibility and Controlling) were 40.23±6.84, 19.40±3.83, 13.79±4.04, 12.93±3.46 respectively. A statistically significant difference was found between the nurses' gender, educational status, the unit they worked in, the knowledge scores of the equipment used in the monitoring of vital signs in this unit and the status of monitoring the night vital signs of the health professional (p<0.05). Conclusion: It was observed that the nurses' knowledge about night vital signs monitoring was moderate, some individual characteristics affected their views on night vital signs monitoring. In this context, it can be suggested to organize in-service trainings based on evidence- based guidelines to update the knowledge of nurses working in the clinic on monitoring night vital signs.