Yıl: 2022 Cilt: 49 Sayı: 1 Sayfa Aralığı: 36 - 44 Metin Dili: İngilizce DOI: 10.5798/dicletip.1086124 İndeks Tarihi: 21-09-2022

Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide

Öz:
Objective: It was aimed to investigate the antigenotoxic effect of Ganoderma lucidum against the damage caused by lipopolysaccharide (LPS) in rat bone marrow cells by micronucleus test and comet analysis. Method: In the experiment, 28 Wistar albino female rats were used and 4 groups were formed. Only physiological saline was applied to the control group. A single dose of 7.5 mg/kg LPS was administered intraperitoneally to the LPS group. LPS+ Ganoderma lucidum (GL): Ganoderma lucidum was given by gavage at a dose of 50 mg/kg per day for one week. Then, on the 8th day, LPS was applied intraperitoneally. Ganoderma lucidum group: GL was given by gavage at 50 mg/kg per day for 1 week. After 24 hours, the rats were euthanized by cervical dislocation under anesthesia. Analyzes were performed for percentage of micronucleated polychromatic erythrocytes (MNPCE) and Polychromatic erythrocytes/Normochromatic erythrocytes (PCE/NCE) ratio and DNA damage in rat femur bone marrow cells. Results: When the percentage of micronucleus and PCE/NCE ratio were examined, no statistical significance was observed when the groups were compared both with the control and among themselves. Compared to the control group, it was observed that the comet parameters of the experimental groups (excluding the head DNA) increased significantly. Length tail (L tail), tail DNA, olive tail moment (OTM) values were significantly different both compared to the control and among themselves. While there was a significant difference between LPS+GL group and LPS and GL groups in Tail Moment (TM) value and Length comet (L comet) value, no difference was observed between LPS and GL groups. Conclusion: When all of the findings is considered, Ganoderma lucidum cannot be classified as a genotoxic agent. We can say, however, that it reverses the genotoxic damage in issue.
Anahtar Kelime:

Lipopolisakkarit ile İndüklenen Sıçan Kemik İliğinde Ganoderma Lucidum'un Genotoksik ve Sitotoksik Aktivitesinin Değerlendirilmesi

Öz:
Amaç: Sıçan kemik iliği hücrelerinde lipopolisakkaritin (LPS) oluşturduğu hasara karşı Ganoderma lucidum’un antigenotoksik etkisi mikronükleus testi ve komet analizi ile araştırılması amaçlanmıştır. Yöntemler: Deneyde 28 adet Wistar albino dişi sıçanlar kullanılarak ve 4 grup oluşturuldu. Kontrol grubuna sadece serum fizyolojik uygulandı. LPS grubuna, intraperitoneal olarak tek doz 7.5 mg/kg LPS uygulandı. LPS+ Ganoderma lucidum (GL): Ganoderma lucidum bir hafta süreyle günde 50 mg/kg dozda gavaj yoluyla verildi. Daha sonra 8. gün intraperitoneal olarak LPS uygulandı. Ganoderma lucidum grubu: GL 1 hafta boyunca günde 50 mg/kg olacak şeklide gavaj ile verildi. 24 saat sonra sıçanlar anestezi eşliğinde servikal dislokasyonla ötanazi edildi. Sıçan femur kemik iliği hücrelerinde, mikronükleuslu polikromatik eritrositlerin (MNPCE) yüzdesi, Polikromatik eritrositlerin/Normokromatik eritrositlere (PCE/NCE) oranı ve DNA hasarı için analizler yapıldı. Bulgular: Mikronükleus yüzdesi ve PCE/NCE oranı incelendiğinde gruplar hem kontrol ile hem de kendi aralarında kıyaslandığında isatatistiksel olarak anlamlılık gözlenmedi. Kontrol grubuna göre deney gruplarının kuyruklu yıldız parametreleri (baş DNA hariç) önemli derecede arttığı görüldü. Kuyruk uzunluğu (L tail), kuyruk DNA, olive tail moment (OTM) değerleri hem kontrole göre hem de kendi aralarında anlamlı derecede farklıydı. Kuyruk momenti (TM) değeri ve comet uzunluğu (L comet) değerinde LPS+GL grubu ile LPS ve GL grupları arasında anlamlı fark bulunurken, LPS ve GL grupları arasında fark gözlenmedi. Sonuç: Tüm bulgular değerlendirildiğinde Ganoderma lucidum genotoksik ajan olarak sınıflandırılamaz. Ancak söz konusu genotoksik hasarı tersine çevirdiğini söyleyebiliriz.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA BARAN M, Bitgen N, Kalkan K, Göktepe Ö, Varol S, Yay A (2022). Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. , 36 - 44. 10.5798/dicletip.1086124
Chicago BARAN MÜNEVVER,Bitgen Nazmiye,Kalkan Kübra Tuğçe,Göktepe Özge,Varol Salih,Yay Arzu Hanım Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. (2022): 36 - 44. 10.5798/dicletip.1086124
MLA BARAN MÜNEVVER,Bitgen Nazmiye,Kalkan Kübra Tuğçe,Göktepe Özge,Varol Salih,Yay Arzu Hanım Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. , 2022, ss.36 - 44. 10.5798/dicletip.1086124
AMA BARAN M,Bitgen N,Kalkan K,Göktepe Ö,Varol S,Yay A Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. . 2022; 36 - 44. 10.5798/dicletip.1086124
Vancouver BARAN M,Bitgen N,Kalkan K,Göktepe Ö,Varol S,Yay A Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. . 2022; 36 - 44. 10.5798/dicletip.1086124
IEEE BARAN M,Bitgen N,Kalkan K,Göktepe Ö,Varol S,Yay A "Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide." , ss.36 - 44, 2022. 10.5798/dicletip.1086124
ISNAD BARAN, MÜNEVVER vd. "Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide". (2022), 36-44. https://doi.org/10.5798/dicletip.1086124
APA BARAN M, Bitgen N, Kalkan K, Göktepe Ö, Varol S, Yay A (2022). Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. Dicle Tıp Dergisi, 49(1), 36 - 44. 10.5798/dicletip.1086124
Chicago BARAN MÜNEVVER,Bitgen Nazmiye,Kalkan Kübra Tuğçe,Göktepe Özge,Varol Salih,Yay Arzu Hanım Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. Dicle Tıp Dergisi 49, no.1 (2022): 36 - 44. 10.5798/dicletip.1086124
MLA BARAN MÜNEVVER,Bitgen Nazmiye,Kalkan Kübra Tuğçe,Göktepe Özge,Varol Salih,Yay Arzu Hanım Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. Dicle Tıp Dergisi, vol.49, no.1, 2022, ss.36 - 44. 10.5798/dicletip.1086124
AMA BARAN M,Bitgen N,Kalkan K,Göktepe Ö,Varol S,Yay A Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. Dicle Tıp Dergisi. 2022; 49(1): 36 - 44. 10.5798/dicletip.1086124
Vancouver BARAN M,Bitgen N,Kalkan K,Göktepe Ö,Varol S,Yay A Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. Dicle Tıp Dergisi. 2022; 49(1): 36 - 44. 10.5798/dicletip.1086124
IEEE BARAN M,Bitgen N,Kalkan K,Göktepe Ö,Varol S,Yay A "Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide." Dicle Tıp Dergisi, 49, ss.36 - 44, 2022. 10.5798/dicletip.1086124
ISNAD BARAN, MÜNEVVER vd. "Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide". Dicle Tıp Dergisi 49/1 (2022), 36-44. https://doi.org/10.5798/dicletip.1086124