TY  - JOUR
TI  - Investigation of inhibition effect of folic acid (vitamin B9) on angiotensin-converting enzyme  activity purified from human plasma
AB  - Inhibition of the angiotensin-converting enzyme (ACE, EC 3.4.15.1) is one of the most important hypertension treatments. Here, ACE was purified from human plasma with affinity chromatography. The purity and molecule weight of ACE were identified utilizing the SDS-PAGE and  viewed in two bands at around 60 kDa and 70 kDa on the gel. KM and Vmax constants from the Lineweaver-Burk graphic were computed to be 0.6 mM and 175.44 (µmol/min).mL-1 , respectively. The effects of folic acid (vitamin B9) on purified ACE were studied. Folic acid on purified ACE demonstrated an inhibitory efficacy. The IC50 value for folic acid was calculated to be 127.94 µM.  Kind of inhibitory and Ki constant for folic acid were defined. The kind of inhibitory for folic acid was  found as non-competitive inhibitory. Ki constant was computed to be 226.59 µM for folic acid. In this  study, it was concluded that folic acid, which shows an inhibitor efficacy on ACE, may have both therapeutic and protective impacts against hypertension.
AU  - BAŞ, Zehra
DO  - 10.21597/jist.1109149
PY  - 2022
JO  - Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi
VL  - 12
IS  - 3
SN  - 2146-0574
SP  - 1607
EP  - 1614
DB  - TRDizin
UR  - http://search/yayin/detay/1122982
ER  -