Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro
Yıl: 2022 Cilt: 47 Sayı: 1 Sayfa Aralığı: 13 - 22 Metin Dili: İngilizce DOI: 10.55262/fabadeczacilik.1078759 İndeks Tarihi: 22-09-2022
Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro
Öz: Endocrine-disrupting chemicals like bisphenol A (BPA) and its analogs have negative effects on human health. This research aims to determine the cytotoxic effects of BPA and its four different analogs bisphenol S (BPS), bisphenol F (BPF), bisphenol Z (BPZ), bisphenol AF (BPAF) on both cancer and healthy cell lines simultaneously by performing an MTT test. In this study, human breast cancer cells (MCF-7) and human Sertoli cells (HSeC) were used for cell culture. MCF-7 and HSeC were exposed to BPA, BPS, BPF, BPZ, BPAF for 24 h. After that, the inhibitory effect of Bisphenols (IC50) was determined by measuring the absorbance. While BPF was the least cytotoxic alternative depending on the highest IC50 values in both cell lines, BPZ was found to be the most cytotoxic alternative in HSeC cell line. In the MCF-7 cell line, BPA and BPZ were found to have equally cytotoxic effects.
Anahtar Kelime: Bisfenol A ve Analoglarının MCF-7 ve HSeC Hücre Hatlarında in vitro Sitotoksik Etkisinin Değerlendirilmesi
Öz: Bisfenol A (BPA) gibi endokrin bozucu kimyasallar ve benzerleri insan sağlığı üzerinde olumsuz etkilere sahiptir. Bu araştırma, BPA ve dört farklı analogunun bisphenol S (BPS), bisfenol F (BPF), bisfenol Z (BPZ), bisfenol AF (BPAF) hem kanser hem de sağlıklı hücre hatları üzerindeki sitotoksik etkilerini MTT testi yaparak aynı anda belirlemeyi amaçlamaktadır. Bu çalışmada, hücre kültürü için insan meme kanseri hücreleri (MCF-7) ve insan sertoli hücreleri (HSeC) kullanılmıştır. MCF-7 ve HSeC, 24 saat BPA, BPS, BPF, BPZ, BPAF’ye maruz bırakılmıştır. Daha sonra, bisfenollerin (IC50) inhibitör etkisi absorbans ölçümü ile belirlenmiştir. En yüksek IC50 değerleri nedeniyle her iki hücre hattında BPF en az sitotoksik alternatif iken, BPZ’nin HSeC hücre hattında en sitotoksik alternatif olduğu bulunmuştur. MCF-7 hücre hattında, BPA ve BPZ’nin eşit sitotoksik etkilere sahip olduğu bulunmuştur.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | İPEK S, İYİGÜNDOĞDU İ, ÜSTÜNDAG A, Duydu Y (2022). Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. , 13 - 22. 10.55262/fabadeczacilik.1078759 |
| Chicago | İPEK Seda,İYİGÜNDOĞDU İREM,ÜSTÜNDAG AYLIN,Duydu Yalçın Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. (2022): 13 - 22. 10.55262/fabadeczacilik.1078759 |
| MLA | İPEK Seda,İYİGÜNDOĞDU İREM,ÜSTÜNDAG AYLIN,Duydu Yalçın Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. , 2022, ss.13 - 22. 10.55262/fabadeczacilik.1078759 |
| AMA | İPEK S,İYİGÜNDOĞDU İ,ÜSTÜNDAG A,Duydu Y Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. . 2022; 13 - 22. 10.55262/fabadeczacilik.1078759 |
| Vancouver | İPEK S,İYİGÜNDOĞDU İ,ÜSTÜNDAG A,Duydu Y Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. . 2022; 13 - 22. 10.55262/fabadeczacilik.1078759 |
| IEEE | İPEK S,İYİGÜNDOĞDU İ,ÜSTÜNDAG A,Duydu Y "Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro." , ss.13 - 22, 2022. 10.55262/fabadeczacilik.1078759 |
| ISNAD | İPEK, Seda vd. "Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro". (2022), 13-22. https://doi.org/10.55262/fabadeczacilik.1078759 |
| APA | İPEK S, İYİGÜNDOĞDU İ, ÜSTÜNDAG A, Duydu Y (2022). Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. FABAD Journal of Pharmaceutical Sciences, 47(1), 13 - 22. 10.55262/fabadeczacilik.1078759 |
| Chicago | İPEK Seda,İYİGÜNDOĞDU İREM,ÜSTÜNDAG AYLIN,Duydu Yalçın Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. FABAD Journal of Pharmaceutical Sciences 47, no.1 (2022): 13 - 22. 10.55262/fabadeczacilik.1078759 |
| MLA | İPEK Seda,İYİGÜNDOĞDU İREM,ÜSTÜNDAG AYLIN,Duydu Yalçın Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. FABAD Journal of Pharmaceutical Sciences, vol.47, no.1, 2022, ss.13 - 22. 10.55262/fabadeczacilik.1078759 |
| AMA | İPEK S,İYİGÜNDOĞDU İ,ÜSTÜNDAG A,Duydu Y Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. FABAD Journal of Pharmaceutical Sciences. 2022; 47(1): 13 - 22. 10.55262/fabadeczacilik.1078759 |
| Vancouver | İPEK S,İYİGÜNDOĞDU İ,ÜSTÜNDAG A,Duydu Y Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. FABAD Journal of Pharmaceutical Sciences. 2022; 47(1): 13 - 22. 10.55262/fabadeczacilik.1078759 |
| IEEE | İPEK S,İYİGÜNDOĞDU İ,ÜSTÜNDAG A,Duydu Y "Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro." FABAD Journal of Pharmaceutical Sciences, 47, ss.13 - 22, 2022. 10.55262/fabadeczacilik.1078759 |
| ISNAD | İPEK, Seda vd. "Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro". FABAD Journal of Pharmaceutical Sciences 47/1 (2022), 13-22. https://doi.org/10.55262/fabadeczacilik.1078759 |
