TY  - JOUR
TI  - The impact of oocyte denudation without a pre-incubation on  intracytoplasmic sperm injection outcomes
AB  - Aims: Debate over the optimal timing of oocyte denudation following oocyte retrieval  continues. Multinucleation has been associated with lower embryo quality and implantation  rate. Defining oocyte characteristics might increase the chances of better embryo selection.  This study aimed to investigate the laboratory and clinical outcomes of intracytoplasmic sperm  injection when the oocytes were denuded immediately after oocyte retrieval. Methods: A prospective randomized sibling-oocyte study was performed. The participants were  under 40 years of age with more than 5 oocytes. Patients with male factors were excluded. The  study and control groups were formed by simple randomization. The study group underwent  oocyte denudation immediately. In the control group, oocytes were routinely incubated in an  equilibration solution for 2 h until the removal of the cumulus cells. Outcome measures were  normal fertilization (two pronuclei, 2PN), multinucleation rate, the proportion of good quality  embryos, blastocyst formation, and pregnancy rate. Results: A total of 792 oocytes were collected from 54 participants (mean age: 30.6±3.7 years).  The fertilization rate was higher in the study group (n=209 in 376) compared with the controls  (n=201 in 416) (55.6% vs. 48.3%, p=0.041). The multinucleation rate (6.6% vs. 4.3%, p=0.150),  proportion of grade 1 embryos on day 3 (48.7% vs. 43%, p=0.112) and day 5 (27.7% vs. 23.8%,  p=0.214), the proportion of embryos reaching blastocyst stage (34.0% vs. 28.1%, p=0.072), and  the pregnancy rates (78.6% vs. 71.4%, p=1.000) were similar. Conclusions: Our results demonstrated that immediate removal of cumulus cells does not have  any negative impact on intracytoplasmic sperm injection outcomes.
AU  - Kanaat, Şenay
AU  - Dönmez Çakıl, Yaprak
AU  - Selam, Belgin
AU  - Düzgün, Zehra Beyza
AU  - CINCIK, MEHMET
DO  - 10.4274/gulhane.galenos.2022.59454
PY  - 2022
JO  - Gülhane Tıp Dergisi
VL  - 64
IS  - 3
SN  - 1302-0471
SP  - 235
EP  - 239
DB  - TRDizin
UR  - http://search/yayin/detay/1124087
ER  -