Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline
Yıl: 2022 Cilt: 10 Sayı: 2 Sayfa Aralığı: 929 - 939 Metin Dili: İngilizce DOI: 10.29130/dubited.844331 İndeks Tarihi: 27-09-2022
Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline
Öz: Because of its excellent electrochemical properties, extreme redox performance, and ability to mediate the electron transfer between the electrode surface and the reaction site, polyaniline (PANI) is one of the most ideal and well-known conductive polymers for biosensor design. This research developed an electrochemical enzymatic biosensor system with PANI film-coated screen printed electrodes (SPE) using the one-step direct electropolymerization process. PANI electropolymerization was performed in different acidic solutions, and the effects on the electrodeposition of the potential range, potential scan rate, and cycle number are discussed depending on these acidic solutions. The surface morphologies of films prepared with different processes were characterized by usıng the SEM (scanning electron microscopy) technique. A sensitive and selective catechol biosensor was developed by immobilizing the tyrosinase (Tyr) enzyme into PANI film combined with glutaraldehyde as a cross-linking agent. After optimizing the biosensor performance conditions, the developed biosensor measured catechol in green tea samples.
Anahtar Kelime: Farklı Elektropolimerizasyon Koşulları Kullanılarak Hazırlanan Polianilin ile Elektrokimyasal Biyosensör Geliştirilmesi
Öz: Polianilin (PANI), mükemmel elektrokimyasal özellikleri, etkili redoks davranışı ve reaksiyon esnasında elektrot yüzeyine elektron transferine aracılık etme kabiliyeti nedeniyle nedeniyle biyosensör tasarımı için en çok tercih edilen ve bilinen iletken polimerlerden biridir. Bu çalışmada, doğrudan elektropolimerizasyon (tek aşamalı) işlemi kullanılarak hazırlanan PANI film kaplı yüzey baskılı elektrotlar (SPE) ile elektrokimyasal bir enzimatik biyosensör sistemi geliştirilmiştir. Çalışmada, PANI elektropolimerizasyonu farklı asidik çözeltilerde gerçekleştirilmiş ve potansiyel aralığın, potansiyel tarama hızının ve döngü sayısının elektrodepozisyona etkileri gözlemlenmiştir. Farklı işlemlerle hazırlanan filmlerin yüzey morfolojileri, taramalı elektron mikroskobu (SEM) tekniği ile karakterize edilmiştir. Glutaraldehit ile çapraz bağlanma tekniği ile tirozinaz (Tyr) enziminin PANI filmine immobilize edilmesiyle oldukça kararlı ve etkili bir katekol biyosensörü hazırlanmıştır. Biyosensör performans koşullarının optimizasyonundan sonra, yeşil çay örneklerinde katekol analizi için geliştirilen biyosensör kullanılmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | YILDIRIM-TIRGIL N, KALKAN Z, Öztürk S, sürdem s, Oktem M (2022). Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. , 929 - 939. 10.29130/dubited.844331 |
| Chicago | YILDIRIM-TIRGIL NIMET,KALKAN Zeycan,Öztürk Soner,sürdem sedat,Oktem Mehmet Fatih Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. (2022): 929 - 939. 10.29130/dubited.844331 |
| MLA | YILDIRIM-TIRGIL NIMET,KALKAN Zeycan,Öztürk Soner,sürdem sedat,Oktem Mehmet Fatih Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. , 2022, ss.929 - 939. 10.29130/dubited.844331 |
| AMA | YILDIRIM-TIRGIL N,KALKAN Z,Öztürk S,sürdem s,Oktem M Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. . 2022; 929 - 939. 10.29130/dubited.844331 |
| Vancouver | YILDIRIM-TIRGIL N,KALKAN Z,Öztürk S,sürdem s,Oktem M Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. . 2022; 929 - 939. 10.29130/dubited.844331 |
| IEEE | YILDIRIM-TIRGIL N,KALKAN Z,Öztürk S,sürdem s,Oktem M "Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline." , ss.929 - 939, 2022. 10.29130/dubited.844331 |
| ISNAD | YILDIRIM-TIRGIL, NIMET vd. "Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline". (2022), 929-939. https://doi.org/10.29130/dubited.844331 |
| APA | YILDIRIM-TIRGIL N, KALKAN Z, Öztürk S, sürdem s, Oktem M (2022). Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 10(2), 929 - 939. 10.29130/dubited.844331 |
| Chicago | YILDIRIM-TIRGIL NIMET,KALKAN Zeycan,Öztürk Soner,sürdem sedat,Oktem Mehmet Fatih Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. Düzce Üniversitesi Bilim ve Teknoloji Dergisi 10, no.2 (2022): 929 - 939. 10.29130/dubited.844331 |
| MLA | YILDIRIM-TIRGIL NIMET,KALKAN Zeycan,Öztürk Soner,sürdem sedat,Oktem Mehmet Fatih Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, vol.10, no.2, 2022, ss.929 - 939. 10.29130/dubited.844331 |
| AMA | YILDIRIM-TIRGIL N,KALKAN Z,Öztürk S,sürdem s,Oktem M Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2022; 10(2): 929 - 939. 10.29130/dubited.844331 |
| Vancouver | YILDIRIM-TIRGIL N,KALKAN Z,Öztürk S,sürdem s,Oktem M Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2022; 10(2): 929 - 939. 10.29130/dubited.844331 |
| IEEE | YILDIRIM-TIRGIL N,KALKAN Z,Öztürk S,sürdem s,Oktem M "Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline." Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 10, ss.929 - 939, 2022. 10.29130/dubited.844331 |
| ISNAD | YILDIRIM-TIRGIL, NIMET vd. "Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 10/2 (2022), 929-939. https://doi.org/10.29130/dubited.844331 |
