The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model

bostancı, meriç emre; TAŞ, Ayca; Silig, Yavuz; KOÇ, TÜLAY; GÜRSOY, SİNAN; Avcı, Onur

doi:10.26650/experimed.1102002

The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model

Meriç Emre BOSTANCI, (Sivas Numune Hastanesi Cerrahi Onkoloji Anabilim Dalı, Sivas, Türkiye)

Onur AVCI, (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Sivas, Türkiye)

Sinan GÜRSOY, (Sivas Cumhuriyet Üniversitesi Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü, Sivas, Türkiye)

AYÇA TAŞ, (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı, Sivas, Türkiye)

Tülay KOÇ, (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Sivas, Türkiye)

YAVUZ SİLİĞ (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Sivas, Türkiye)

EXPERIMED

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Yıl: 2022 Cilt: 12 Sayı: 2 Sayfa Aralığı: 66 - 73 Metin Dili: İngilizce DOI: 10.26650/experimed.1102002 İndeks Tarihi: 28-09-2022

The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model

Öz:

Objective: The aim of this study was to examine the effect of rectal ozone on the histopathological healing of the colonic mucosa, tissue oxidative stress, and bacterial translocation in the experimental colitis model. Materials and Methods: Three groups of rats were randomly formed [Group 1: Sham group, group 2: Control group, group 3: Ozone treatment group]. Microscopic and macroscopic scoring were done histopathologically in all groups. Glutathione-s-transferase (GST), superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) levels were measured in the colon and liver tissue. Blood cultures were taken for the detection of bacterial translocation. Results: Microscopic damage scores were found as 0.0 (0.0-2.0) in the sham group, 3.0 (3.0-3.0) in the control group, 2.0 (0.0-2.0) in the ozone treatment group (p=0.001). Macroscopic damage scores were found as 0.0 (0.0-1.0) in the sham group, 3.0 (0.0-4.0) in the control group, 0.0 (0.0-1.0) in the ozone treatment group; the scores of ozone treatment and sham groups were found to be statistically different (p=0.004). Compared to the control group, the bacterial translocation in the liver, mesenteric lymph node, portal vein, spleen, and systemic blood was fewer in the ozone treatment group. Statistically significant differences were also observed between the groups’ SOD and GST levels in colon tissue and MDA, SOD, and GST levels in liver tissue. Regarding MDA values in the liver tissue of the groups, it was 1.95±0.43 in Group I, 3.63±0.81 in Group II, and 1.19±0.72 U/mg in Group III (p=0.017). When the liver tissue SOD levels of the groups were examined, it was 8.21±0.76 U/mg in Group I, 4.57±0.58 U/mg in Group II, and 8.62±1.13 U/mg in Group III (p=0.029). When GST values in liver tissue belonging to the groups were examined, 0.35±0.16 in Group 1, 0.23±0.03 in Group II, 0.49±0.13 U/mg in Group III (p=0.036). Conclusion: Rectal ozone application plays a role in increasing the organism’s antioxidant activity and has an effective role in increasing the enzyme activities of antioxidant defenses. In addition, rectal ozone application has a positive effect on wound healing at a histopathological level and reduces bacterial translocation in various tissues.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	bostancı m, Avcı O, GÜRSOY S, TAŞ A, KOÇ T, Silig Y (2022). The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. , 66 - 73. 10.26650/experimed.1102002
Chicago	bostancı meriç emre,Avcı Onur,GÜRSOY SİNAN,TAŞ Ayca,KOÇ TÜLAY,Silig Yavuz The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. (2022): 66 - 73. 10.26650/experimed.1102002
MLA	bostancı meriç emre,Avcı Onur,GÜRSOY SİNAN,TAŞ Ayca,KOÇ TÜLAY,Silig Yavuz The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. , 2022, ss.66 - 73. 10.26650/experimed.1102002
AMA	bostancı m,Avcı O,GÜRSOY S,TAŞ A,KOÇ T,Silig Y The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. . 2022; 66 - 73. 10.26650/experimed.1102002
Vancouver	bostancı m,Avcı O,GÜRSOY S,TAŞ A,KOÇ T,Silig Y The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. . 2022; 66 - 73. 10.26650/experimed.1102002
IEEE	bostancı m,Avcı O,GÜRSOY S,TAŞ A,KOÇ T,Silig Y "The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model." , ss.66 - 73, 2022. 10.26650/experimed.1102002
ISNAD	bostancı, meriç emre vd. "The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model". (2022), 66-73. https://doi.org/10.26650/experimed.1102002

APA	bostancı m, Avcı O, GÜRSOY S, TAŞ A, KOÇ T, Silig Y (2022). The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. EXPERIMED, 12(2), 66 - 73. 10.26650/experimed.1102002
Chicago	bostancı meriç emre,Avcı Onur,GÜRSOY SİNAN,TAŞ Ayca,KOÇ TÜLAY,Silig Yavuz The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. EXPERIMED 12, no.2 (2022): 66 - 73. 10.26650/experimed.1102002
MLA	bostancı meriç emre,Avcı Onur,GÜRSOY SİNAN,TAŞ Ayca,KOÇ TÜLAY,Silig Yavuz The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. EXPERIMED, vol.12, no.2, 2022, ss.66 - 73. 10.26650/experimed.1102002
AMA	bostancı m,Avcı O,GÜRSOY S,TAŞ A,KOÇ T,Silig Y The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. EXPERIMED. 2022; 12(2): 66 - 73. 10.26650/experimed.1102002
Vancouver	bostancı m,Avcı O,GÜRSOY S,TAŞ A,KOÇ T,Silig Y The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model. EXPERIMED. 2022; 12(2): 66 - 73. 10.26650/experimed.1102002
IEEE	bostancı m,Avcı O,GÜRSOY S,TAŞ A,KOÇ T,Silig Y "The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model." EXPERIMED, 12, ss.66 - 73, 2022. 10.26650/experimed.1102002
ISNAD	bostancı, meriç emre vd. "The Effect of Rectal Ozone Use on Bacterial Translocation and Oxidative Stress in Experimental Colitis Model". EXPERIMED 12/2 (2022), 66-73. https://doi.org/10.26650/experimed.1102002