TY  - JOUR
TI  - Cytotoxic and Apoptotic Effects of the Combination  of Borax (Sodium Tetraborate) and 5-Fluorouracil on  DLD-1 Human Colorectal Adenocarcinoma Cell Line
AB  - Objectives: Colorectal cancer (CRC) remains a crucial health problem due to the toxicity of 5-Fluorouracil (5-FU) as first-line chemotherapy  agent for treating CRC. The anticancer effects of boron and its compounds have been shown in various cell lines. This study aimed to examine the  cytotoxic and apoptotic effects of borax (sodium tetraborate) alone or along with 5-FU on human CRC cells, DLD-1. Materials and Methods: Cytotoxicity and apoptosis were determined by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay,  4’,6-diamidino-2-phenylindole and annexin V/propidium iodide staining. Results: The results showed that combined treatment revealed a significant time- and concentration-dependent cytotoxic effect on DLD-1 cells  compared with borax or 5-FU treatment alone. The combination of borax and 5-FU induced a clear increase in the early apoptotic cell percentage,  compared to the cells treated with monotherapies. Additionally, a significant increase in condensed and fragmented nuclei was detected in DLD-1  cells treated with the combination treatment compared with borax or 5-FU alone. Conclusion: Our current findings suggest that the combination of borax with 5-FU has a strong cytotoxic and apoptotic effect on the human CRC  DLD-1 cells
AU  - Kaya-Sezginer, Ecem
AU  - KIRLANGIÇ, Ömer Faruk
AU  - Yavuz, Ozlem
AU  - Gur, Serap
AU  - Ören, Sema
DO  - 10.4274/tjps.galenos.2021.29726
PY  - 2022
JO  - Turkish Journal of Pharmaceutical Sciences
VL  - 19
IS  - 4
SN  - 1304-530X
SP  - 371
EP  - 376
DB  - TRDizin
UR  - http://search/yayin/detay/1125617
ER  -