UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri
Yıl: 2022 Cilt: 48 Sayı: 2 Sayfa Aralığı: 255 - 260 Metin Dili: Türkçe DOI: 10.32708/uutfd.1148449 İndeks Tarihi: 29-09-2022
UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri
Öz: Makrofajlar immün sistemin önemli hücreleridir ve eksiklikleri ya da disfonksiyonları vücutta ciddi hastalıklara yol açar. Bir pirimidin nükleotidi olan üridin-5’-trifosfat’ın (UTP) makrofajlar için bir sinyal molekülü gibi davrandığı bilinmektedir. Ancak UTP’nin makrofaj canlılığı ya da proliferasyonu üzerine etkileri henüz araştırılmamıştır. Dolayısıyla bu çalışmada farklı konsantrasyonlarda (1, 10 ve 100 μM) uygulanan UTP’nin RAW 264.7 fare makrofaj hücrelerinin canlılığı üzerine etkileri farklı zaman noktalarında MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür] testi kullanılmak suretiyle incelenmiştir. UTP uygulandıktan 24 ya da 48 saat sonra hücre canlılığında kontrole göre herhangi bir fark gözlenmezken, 10 μM konsantrasyonda uygulanan UTP’nin 72 saat sonra hücre canlılığını kontrole göre %27,7 oranında (p
Anahtar Kelime: Effects of UTP on Viability in RAW 264.7 Cell Line
Öz: Macrophages are important cells of immune system and their deficiency or dysfunction lead to severe diseases. Uridine-5’-triphosphate, a pyrimidine nucleotide, is known to behave as a signaling molecule for macrophages. However, the effect of UTP on macrophage viability or proliferation is yet to be researched. Therefore, we investigated the effects of different concentrations (1, 10 and 100 μM) of UTP on RAW 264.7 mouse macrophage cell viability at different time points using MTT [(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)] assay. We found that while there was no difference among groups at 24 or 48 hours following UTP administration, cell viability was enhanced by 27,7% (p
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Ermiş E, Cansev Z, etgu O, Yoyen-Ermis D, ORAL H (2022). UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. , 255 - 260. 10.32708/uutfd.1148449 |
| Chicago | Ermiş Erkan,Cansev Zeynep,etgu Onur,Yoyen-Ermis Digdem,ORAL HALUK BARBAROS UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. (2022): 255 - 260. 10.32708/uutfd.1148449 |
| MLA | Ermiş Erkan,Cansev Zeynep,etgu Onur,Yoyen-Ermis Digdem,ORAL HALUK BARBAROS UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. , 2022, ss.255 - 260. 10.32708/uutfd.1148449 |
| AMA | Ermiş E,Cansev Z,etgu O,Yoyen-Ermis D,ORAL H UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. . 2022; 255 - 260. 10.32708/uutfd.1148449 |
| Vancouver | Ermiş E,Cansev Z,etgu O,Yoyen-Ermis D,ORAL H UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. . 2022; 255 - 260. 10.32708/uutfd.1148449 |
| IEEE | Ermiş E,Cansev Z,etgu O,Yoyen-Ermis D,ORAL H "UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri." , ss.255 - 260, 2022. 10.32708/uutfd.1148449 |
| ISNAD | Ermiş, Erkan vd. "UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri". (2022), 255-260. https://doi.org/10.32708/uutfd.1148449 |
| APA | Ermiş E, Cansev Z, etgu O, Yoyen-Ermis D, ORAL H (2022). UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 48(2), 255 - 260. 10.32708/uutfd.1148449 |
| Chicago | Ermiş Erkan,Cansev Zeynep,etgu Onur,Yoyen-Ermis Digdem,ORAL HALUK BARBAROS UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. Uludağ Üniversitesi Tıp Fakültesi Dergisi 48, no.2 (2022): 255 - 260. 10.32708/uutfd.1148449 |
| MLA | Ermiş Erkan,Cansev Zeynep,etgu Onur,Yoyen-Ermis Digdem,ORAL HALUK BARBAROS UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. Uludağ Üniversitesi Tıp Fakültesi Dergisi, vol.48, no.2, 2022, ss.255 - 260. 10.32708/uutfd.1148449 |
| AMA | Ermiş E,Cansev Z,etgu O,Yoyen-Ermis D,ORAL H UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. Uludağ Üniversitesi Tıp Fakültesi Dergisi. 2022; 48(2): 255 - 260. 10.32708/uutfd.1148449 |
| Vancouver | Ermiş E,Cansev Z,etgu O,Yoyen-Ermis D,ORAL H UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri. Uludağ Üniversitesi Tıp Fakültesi Dergisi. 2022; 48(2): 255 - 260. 10.32708/uutfd.1148449 |
| IEEE | Ermiş E,Cansev Z,etgu O,Yoyen-Ermis D,ORAL H "UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri." Uludağ Üniversitesi Tıp Fakültesi Dergisi, 48, ss.255 - 260, 2022. 10.32708/uutfd.1148449 |
| ISNAD | Ermiş, Erkan vd. "UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri". Uludağ Üniversitesi Tıp Fakültesi Dergisi 48/2 (2022), 255-260. https://doi.org/10.32708/uutfd.1148449 |
