The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions

TARI, Canan; ONCU, Şelale; ÖZKAN, Kamer; AVCI, Tuba

The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions

Canan TARI, (İzmir Yüksek Teknoloji Enstitüsü, Gıda Mühendisliği Bölümü, İzmir, Türkiye)

Kamer ÖZKAN, (İzmir Yüksek Teknoloji Enstitüsü, İzmir, Türkiye)

Şelale ONCU, (İzmir Yüksek Teknoloji Enstitüsü, Gıda Mühendisliği Bölümü, İzmir, Türkiye)

Tuba AVCI (İzmir Yüksek Teknoloji Enstitüsü, İzmir, Türkiye)

GIDA

3 0

Yıl: 2011 Cilt: 36 Sayı: 1 Sayfa Aralığı: 25 - 31 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions

Öz:

Bu çalışmada, ortam kompozisyonunu belirleyen değişik faktörlerin Rhizopus oryzae pellet morfolojisine ve poligalakturonaz (PG) enzimi üretimine olan etkisi incelenmiştir. En yüksek PG aktivitesine (11.53 U/ml) 4 mg/kg Zn+2 içeren, Mg+2 içermeyen ve pH 3 olan basal ortamında ulaşılmıştır. Karbon kaynağı olarak 14.78 g/l glukoz, 10 g/l galaktoz, 5 g/l mannoz, 0.5 g/l arabinoz ve 19.73 g/l ksilozun birlikte kullanılmıyla da en yüksek PG aktivitesine (27.94 U/ml) ulaşılmıştır. Mısır unu nitrojen kaynağı olarak PG sentezini tetiklemede oldukça etkili olup bir diğer yüksek tetikleyici olan mısır maserasyon sıvısı (CSL) ve $(NH _4)2SO _4$’den %33 daha fazla aktivite vermiştir. $(NH _4)2SO _4$ and 25 g/l glukoz içereren formulasyon ortalama çapı 1.25± 0.25 olan en fazla sayıda pellet oluşumunu sağlamıştır. Ortalama çapı 1.75±0.25 mm olan pelletlerin oluşumu ise yüksek glukoz konsantrasyonuna sahip (50g/l) maya ekstraktı içeren formulasyon ile elde edilmiştir. Uygun karbon kaynağının (glukoz) uygun nitrojen kaynağı (mısır unu) ile interaktif etkisi PG aktivitesini basal ortama göre 4 kat daha arttırmış, ortalama çapı 1.44±0.35mm olan pelletler oluşturmuştur.

Anahtar Kelime: pelet kültür ortamı Rhizopus oryzae morfoloji poligalakturonaz

Konular: Gıda Bilimi ve Teknolojisi

Değişik ortam komposizyonunda Rhizopus oryzae’nin poligalakturonaz üretimi ile pellet morfolojisinin ilişkisi

Öz:

Various media parameters affecting the polygalacturonase (PG) production of Rhizopus oryzae, were studied and their relation to pellet morphology was investigated. The basal medium in the absence of Mg+2 and in the presence of 4 mg/kg of Zn +2 at pH 3, resulted into maximum PG activity (11.53 U/ml). A composition of 14.78 g/l of glucose, 10 g/l of galactose, 5 g/l mannose, 0.5 g/l of arabinose and 19.73 g/l of xylose resulted into maximum PG activity (27.94 U/ml) when used as combined carbon sources. Corn meal as the nitrogen source, promoted PG synthesis where it resulted into 33 % more activity than corn steep liquor (CSL) and $(NH _4)2SO _4$ which were the next highest promoter. The highest number of pellets with an average mean diameter of 1.25±0.25 mm was obtained with the formulation containing $(NH _4)2SO _4$ and 25 g/l of glucose. Yeast extract on the other hand resulted into pellet formation with an average mean diameter of 1.75±0.25 mm at higher glucose concentration (50 g/l). The interactive effect of suitable carbon source (glucose) with suitable nitrogen source (corn meal) enhanced the PG activity 4 times more than the basal medium with pellets of 1.44±0.35mm in average diameter.

Anahtar Kelime: Rhizopus oryzae morphology polygalacturonase pellets culture media

Konular: Gıda Bilimi ve Teknolojisi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TARI C, ÖZKAN K, ONCU Ş, AVCI T (2011). The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. , 25 - 31.
Chicago	TARI Canan,ÖZKAN Kamer,ONCU Şelale,AVCI Tuba The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. (2011): 25 - 31.
MLA	TARI Canan,ÖZKAN Kamer,ONCU Şelale,AVCI Tuba The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. , 2011, ss.25 - 31.
AMA	TARI C,ÖZKAN K,ONCU Ş,AVCI T The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. . 2011; 25 - 31.
Vancouver	TARI C,ÖZKAN K,ONCU Ş,AVCI T The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. . 2011; 25 - 31.
IEEE	TARI C,ÖZKAN K,ONCU Ş,AVCI T "The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions." , ss.25 - 31, 2011.
ISNAD	TARI, Canan vd. "The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions". (2011), 25-31.

APA	TARI C, ÖZKAN K, ONCU Ş, AVCI T (2011). The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. GIDA, 36(1), 25 - 31.
Chicago	TARI Canan,ÖZKAN Kamer,ONCU Şelale,AVCI Tuba The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. GIDA 36, no.1 (2011): 25 - 31.
MLA	TARI Canan,ÖZKAN Kamer,ONCU Şelale,AVCI Tuba The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. GIDA, vol.36, no.1, 2011, ss.25 - 31.
AMA	TARI C,ÖZKAN K,ONCU Ş,AVCI T The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. GIDA. 2011; 36(1): 25 - 31.
Vancouver	TARI C,ÖZKAN K,ONCU Ş,AVCI T The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions. GIDA. 2011; 36(1): 25 - 31.
IEEE	TARI C,ÖZKAN K,ONCU Ş,AVCI T "The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions." GIDA, 36, ss.25 - 31, 2011.
ISNAD	TARI, Canan vd. "The relationship of pellet morphology to polygalacturonase production of Rhizopus oryzae in various media compositions". GIDA 36/1 (2011), 25-31.