TY  - JOUR
TI  - Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey
AB  - Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms. Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used. Results: In the tests performed, approximately 1.2 kb of DNA fragments specific to phytoplasma were obtained. The 16S rRNA nucleotide sequence (1254 bp in length) (OM663745) revealed that it was showed more than 99.44% nucleotide similarity to other ‘Ca. P. trifolii’ members. The tentative RFLP and phylogenetic analyzes performed proved the  ‘Ca. P. trifolii’ the infection from the Clover proliferation group (16SrVI) group and subgroup A in symptomatic pepper plants. Conclusion: The presence of ‘Ca. P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.
AU  - güller, abdullah
AU  - Demirel, Serap
AU  - KURT, Zeynelabidin
AU  - Korkmaz, Gülüstan
AU  - usta, mustafa
DO  - 10.20289/zfdergi.1081067
PY  - 2022
JO  - Ege Üniversitesi Ziraat Fakültesi Dergisi
VL  - 59
IS  - 3
SN  - 1018-8851
SP  - 429
EP  - 437
DB  - TRDizin
UR  - http://search/yayin/detay/1126386
ER  -