Objectives: Histologic grade is the most useful predictor of prognosis of prostate cancer. The Gleason scoring is the only grading method for prostatic carcinoma, which is recommended by World Health Organization. The Gleason grading (3+3) 6 is the lowest score currently assigned. In radical materials, we can see all prostate areas and decide certain grading easily. However, in the biopsy, we can see only a part of the tumor and we cannot be sure of the histologic grade of the total tumor. If the tumor has a potential for cribriform formation, we cannot detect it with current methods. Our aim in this study was to detect molecular heterogeneity in well-differentiated prostate cancers. Materials and Methods: Twenty-six prostate needle biopsies from patients with prostate carcinoma Gleason (3+3) were stained and analyzed with immunohistochemical markers of Betacatenin, Cyclin D1, Pax2 and Pax8. Results: While intense staining was observed with Betacatenin in tumor, no significant findings were detected with Cyclin D1, Pax2 and Pax8. Conclusion: Betacatenin, Cyclin D1, Pax2 and Pax8 are the markers that we use frequently in daily pathology practice and have also been the subject of studies in the literature on urogenital system carcinogenesis. However, while intense staining was observed with Betacatenin in well- differentiated tumoral acinar structures, no significant findings were detected with Cyclin D1, Pax2 and Pax8.