Purpose: Universities are one of the main institutions which play important roles in the society. The aim of our study was to evaluate the knowledge, attitudes and behaviors of the employees of Yozgat Bozok University in terms of rational drug use. Materials and Methods: Data was collected by the application of a questionnaire consisting of 4 sections and 41 questions in the electronic environment. All the data was evaluated using descriptive statistical methods. Mann-Whitney U and Kruskall Wallis tests were used to calculate differences between socio-demographic parameters like income, education, occupation, gender and age. Results: The total number of the participants were 189 and %45 were men and %55 were women. Knowledge of rational drug use was found low in the men, in the primary and high school education groups and in the poor income group (p<0.05). Vitamins and analgesics were the main drug groups used without any advice of a physician. Conclusion : Insufficient knowledge of the rational drug use is much more prominent in the low income group, in men and in low educated population but the role of internet is an additional important factor on the drug use which may become much more important in the future. In order to improve the rational drug use, the actions of the organizations and health institutions are expected to consider the role of socio-economic parameters and the internet.