Yıl: 2022 Cilt: 69 Sayı: 2 Sayfa Aralığı: 117 - 122 Metin Dili: İngilizce DOI: 10.33988/auvfd.839517 İndeks Tarihi: 07-10-2022

Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab

Öz:
The demand for the fast-food industry in the world is increasing day by day. In this sense, chicken doner kebab becomes frequently preferred food source in daily life. At the same time, chicken doner kebab is both a good animal origin protein source and a cheaper option. Therefore, it is prepared and consumed in high amounts in Turkey. In this study, it was aimed to determine the presence of Clostridium perfringens and its toxin genes in traditional Turkish chicken doner kebabs purchased from restaurants and modified atmosphere packaged (MAP) samples collected from markets. For this purpose, 100 ready-to-cook and 100 ready-to-eat, totally 200 doner samples have been used as material. As a result, the prevalence of C. perfringens has been found 29%, 6% in readyto- cook and ready-to-eat samples, respectively. The cpa gene was detected in all isolates. However, both cpa and cpe gene was found only in 4% of isolates.
Anahtar Kelime:

Geleneksel türk tavuk döner kebaplarında Clostridium perfringens'in tespiti ve enterotoksin genlerinin (cpa ve cpe) belirlenmesi

Öz:
Dünyada fast-food beslenmeye olan talep her geçen gün artmaktadır. Bu anlamda tavuk döner kebap günlük hayatta sıklıkla tercih edilen bir besin kaynağı haline gelmektedir. Aynı zamanda tavuk döner kebap hem iyi bir hayvansal protein kaynağı hem de daha ekonomik bir seçenek olması nedeniyle Türkiye'de yüksek miktarlarda üretilmekte ve tüketilmektedir. Bu çalışmada, restoranlardan satın alınan geleneksel Türk tipi tavuk döner kebapları ve marketten toplanan modifiye atmosfer paketli (MAP) örneklerde Clostridium perfringens ve toksin genlerinin varlığının belirlenmesi amaçlanmıştır. Bu amaçla 100 adet pişirilmiş ve paketlenmiş ve 100 adet restoranlardan alınmış ve yenmeye hazır olmak üzere toplam 200 döner örneği materyal olarak kullanılmıştır. Sonuç olarak, pişirilmiş ve paketlenmiş örneklerde C. perfringens prevalansı %29 ve restoranlardan alınmış örneklerde ise %6 olarak bulunmuştur. Tüm izolatlarda cpa geni tespit edilmiş olmasına rağmen hem cpa hem de cpe geni izolatların sadece %4'ünde belirlenmiştir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KEVENK T, UYANIK T, Kanat S, ÇADIRCI Ö, gücükoğlu a (2022). Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. , 117 - 122. 10.33988/auvfd.839517
Chicago KEVENK Tahsin Onur,UYANIK TOLGA,Kanat Sibel,ÇADIRCI Özgür,gücükoğlu ali Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. (2022): 117 - 122. 10.33988/auvfd.839517
MLA KEVENK Tahsin Onur,UYANIK TOLGA,Kanat Sibel,ÇADIRCI Özgür,gücükoğlu ali Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. , 2022, ss.117 - 122. 10.33988/auvfd.839517
AMA KEVENK T,UYANIK T,Kanat S,ÇADIRCI Ö,gücükoğlu a Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. . 2022; 117 - 122. 10.33988/auvfd.839517
Vancouver KEVENK T,UYANIK T,Kanat S,ÇADIRCI Ö,gücükoğlu a Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. . 2022; 117 - 122. 10.33988/auvfd.839517
IEEE KEVENK T,UYANIK T,Kanat S,ÇADIRCI Ö,gücükoğlu a "Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab." , ss.117 - 122, 2022. 10.33988/auvfd.839517
ISNAD KEVENK, Tahsin Onur vd. "Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab". (2022), 117-122. https://doi.org/10.33988/auvfd.839517
APA KEVENK T, UYANIK T, Kanat S, ÇADIRCI Ö, gücükoğlu a (2022). Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 69(2), 117 - 122. 10.33988/auvfd.839517
Chicago KEVENK Tahsin Onur,UYANIK TOLGA,Kanat Sibel,ÇADIRCI Özgür,gücükoğlu ali Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. Ankara Üniversitesi Veteriner Fakültesi Dergisi 69, no.2 (2022): 117 - 122. 10.33988/auvfd.839517
MLA KEVENK Tahsin Onur,UYANIK TOLGA,Kanat Sibel,ÇADIRCI Özgür,gücükoğlu ali Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.69, no.2, 2022, ss.117 - 122. 10.33988/auvfd.839517
AMA KEVENK T,UYANIK T,Kanat S,ÇADIRCI Ö,gücükoğlu a Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(2): 117 - 122. 10.33988/auvfd.839517
Vancouver KEVENK T,UYANIK T,Kanat S,ÇADIRCI Ö,gücükoğlu a Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(2): 117 - 122. 10.33988/auvfd.839517
IEEE KEVENK T,UYANIK T,Kanat S,ÇADIRCI Ö,gücükoğlu a "Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 69, ss.117 - 122, 2022. 10.33988/auvfd.839517
ISNAD KEVENK, Tahsin Onur vd. "Detection of Clostridium perfringens and determination of enterotoxin genes (cpa and cpe) in traditional turkish chicken doner kebab". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69/2 (2022), 117-122. https://doi.org/10.33988/auvfd.839517