Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats
Yıl: 2022 Cilt: 69 Sayı: 2 Sayfa Aralığı: 201 - 209 Metin Dili: İngilizce DOI: 10.33988/auvfd.822776 İndeks Tarihi: 07-10-2022
Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats
Öz: The potential protective effects of mesenchymal stem cells (MSCs) on some kidney diseases have been reported. However, the effect of the fetal kidney–derived (FKD)MSCs on doxorubicin-induced nephropathy has not been studied yet. This study aimed to treat rats with doxorubicin-induced kidney injuries by transplantation of –FKD-MSCs. Twenty-four Sprague-Dawley rats were divided into three groups as control, doxorubicin nephropathy (Sham), and doxorubicin + MSC treated group. Serum biochemistry analysis was performed at the beginning and the end of the study. Functional changes in kidneys were evaluated by scintigraphy. In the doxorubicin nephropathy group, histopathological findings such as mesangial cell proliferation, tubular cast, and glomerular hypertrophy were observed, whereas in the MSC group these findings were significantly reduced. CD133 and CD24 positive immunoreactions were the most severe and frequently observed in the MSC group. While positive staining was detected in the tubular epithelium, there was no immunostaining observed in the glomerulus. The results showed that both functional and histological improvements were achieved in the MSC group compared to the Sham group. In conclusion, transplantation of fetal kidney - derived MSCs into patients with renal damage is thought to contribute to the healing of the renal tissue.
Anahtar Kelime: Erkek Sprague-Dawley ratlarda doksorubisin nefropatisinde fetal rat böbreği kökenli mezenkimal kök hücre uygulamasının etkilerinin araştırılması
Öz: Mezenkimal kök hücrelerin (MKH) bazı böbrek hastalıklarındaki potansiyel koruyucu etkileri bildirilmiştir. Bununla birlikte, fetal böbrek kaynaklı (FBK) MKH'ların doksorubisin ile indüklenmiş nefropati üzerindeki etkisi henüz araştırılmamıştır. Bu çalışmanın amacı, doksorubisin kaynaklı böbrek hasarı olan ratlara FBK-MKH'ların transplantasyonu yapılarak hasarın tedavi edilmesidir. Çalışmada yirmi dört adet Sprague–Dawley ırkı rat üç gruba ayrılmıştır. Bunlar: kontrol grubu, doksorubisin nefropatisi (Sham) grubu ve doksorubisin + MKH ile tedavi edilen gruptur. Çalışmanın başında ve sonunda serum biyokimya analizleri gerçekleştirilmiştir. Böbreklerdeki fonksiyonel değişiklikler sintigrafi ile değerlendirilmiştir. Doksorubisin nefropatisi grubunda mezanjiyal hücre proliferasyonu, tübül içi kast birikimi ve glomerüler hipertrofi gibi histopatolojik bulgular gözlenirken, MKH grubunda bu bulgular anlamlı olarak azalmıştır. CD133 ve CD24 pozitif immünreaksiyonlar, en şiddetli ve en sık olarak MKH grubunda gözlenmiştir. Tübüler epitelde pozitif boyanma tespit edilirken glomerulusta immün boyanma gözlenmemiştir. Sonuçlar, Sham grubuna kıyasla MKH grubunda hem fonksiyonel hem de histolojik iyileşmelerin sağlandığını göstermiştir. Sonuç olarak, böbrek hasarı olan hastalara fetal böbrek kaynaklı MKH transplantasyonunun böbrek dokusunun iyileşmesine katkıda bulunduğu düşünülmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Boztok Özgermen B, BULUT G, Alpaslan Pinarli F, GÜLTEKİN S, OZEN D, YAVUZ O, Haydardedeoglu A (2022). Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. , 201 - 209. 10.33988/auvfd.822776 |
| Chicago | Boztok Özgermen Başak,BULUT GAYE,Alpaslan Pinarli Ferda,GÜLTEKİN SALİH SİNAN,OZEN DOGUKAN,YAVUZ ORHAN,Haydardedeoglu Ali Evren Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. (2022): 201 - 209. 10.33988/auvfd.822776 |
| MLA | Boztok Özgermen Başak,BULUT GAYE,Alpaslan Pinarli Ferda,GÜLTEKİN SALİH SİNAN,OZEN DOGUKAN,YAVUZ ORHAN,Haydardedeoglu Ali Evren Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. , 2022, ss.201 - 209. 10.33988/auvfd.822776 |
| AMA | Boztok Özgermen B,BULUT G,Alpaslan Pinarli F,GÜLTEKİN S,OZEN D,YAVUZ O,Haydardedeoglu A Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. . 2022; 201 - 209. 10.33988/auvfd.822776 |
| Vancouver | Boztok Özgermen B,BULUT G,Alpaslan Pinarli F,GÜLTEKİN S,OZEN D,YAVUZ O,Haydardedeoglu A Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. . 2022; 201 - 209. 10.33988/auvfd.822776 |
| IEEE | Boztok Özgermen B,BULUT G,Alpaslan Pinarli F,GÜLTEKİN S,OZEN D,YAVUZ O,Haydardedeoglu A "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats." , ss.201 - 209, 2022. 10.33988/auvfd.822776 |
| ISNAD | Boztok Özgermen, Başak vd. "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats". (2022), 201-209. https://doi.org/10.33988/auvfd.822776 |
| APA | Boztok Özgermen B, BULUT G, Alpaslan Pinarli F, GÜLTEKİN S, OZEN D, YAVUZ O, Haydardedeoglu A (2022). Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 69(2), 201 - 209. 10.33988/auvfd.822776 |
| Chicago | Boztok Özgermen Başak,BULUT GAYE,Alpaslan Pinarli Ferda,GÜLTEKİN SALİH SİNAN,OZEN DOGUKAN,YAVUZ ORHAN,Haydardedeoglu Ali Evren Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi 69, no.2 (2022): 201 - 209. 10.33988/auvfd.822776 |
| MLA | Boztok Özgermen Başak,BULUT GAYE,Alpaslan Pinarli Ferda,GÜLTEKİN SALİH SİNAN,OZEN DOGUKAN,YAVUZ ORHAN,Haydardedeoglu Ali Evren Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.69, no.2, 2022, ss.201 - 209. 10.33988/auvfd.822776 |
| AMA | Boztok Özgermen B,BULUT G,Alpaslan Pinarli F,GÜLTEKİN S,OZEN D,YAVUZ O,Haydardedeoglu A Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(2): 201 - 209. 10.33988/auvfd.822776 |
| Vancouver | Boztok Özgermen B,BULUT G,Alpaslan Pinarli F,GÜLTEKİN S,OZEN D,YAVUZ O,Haydardedeoglu A Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(2): 201 - 209. 10.33988/auvfd.822776 |
| IEEE | Boztok Özgermen B,BULUT G,Alpaslan Pinarli F,GÜLTEKİN S,OZEN D,YAVUZ O,Haydardedeoglu A "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 69, ss.201 - 209, 2022. 10.33988/auvfd.822776 |
| ISNAD | Boztok Özgermen, Başak vd. "Investigation of the effects of fetal rat kidney-derived mesenchymal stem cells implementation on doxorubicin-induced nephropathy in male Sprague–Dawley rats". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69/2 (2022), 201-209. https://doi.org/10.33988/auvfd.822776 |
