Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats
Yıl: 2022 Cilt: 20 Sayı: 2 Sayfa Aralığı: 79 - 85 Metin Dili: İngilizce DOI: 10.4274/tybd.galenos.2021.52523 İndeks Tarihi: 11-10-2022
Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats
Öz: Objective: Corticosteroids are one of the treatment methods used to prevent inflammation in sepsis. This study aimed to determine the anti-inflammatory activity of astaxanthin in sepsis and compare it with dexamethasone. Materials and Methods: After approval of the local ethics committee, 40 Sprague-Dawley male rats were randomly assigned to the control group (n=8), lipopolysaccharide group (n=8), astaxanthin group (n=8), astaxanthin + lipopolysaccharide group (n=8) and dexamethasone + lipopolysaccharide group (n=8). On day 1, these groups were given dimethyl sulfoxide, Salmonella typhimurium lipopolysaccharide, astaxanthin dissolved in dimethyl sulfoxide, astaxanthin and lipopolysaccharide and dexamethasone and lipopolysaccharide, respectively. After 24 hours, rats underwent laparotomy, and liver and blood samples were taken. GraphPad Prism 6 was used for statistical analysis. P values less than 0.05 were considered significant. Results: Nuclear factor-kappa B levels in both treatment groups significantly decreased when compared with the lipopolysaccharide group. Apoptotic cells and reaction severity decreased significantly in the treatment groups compared with the lipopolysaccharide group. Conclusion: This study revealed that the use of astaxanthin had a positive effect on liver tissue undergoing treatment for sepsis. Moreover, despite some differences, measurement values were comparable when dexamethasone was administered.
Anahtar Kelime: Sıçanlarda Lipopolisakkarit ile Oluşturulmuş Sepsis Modelinde Astaksantinin Karaciğer Dokusunda Anti-enflamatuvar Etkilerinin Araştırılması
Öz: Amaç: Kortikosteroidler enflamasyonu önlemek için sepsiste kullanılan tedavi yöntemlerinden biridir. Çalışmamızda astaksantinin sepsiste anti-enflamatuvar aktivitesini göstermeyi ve deksametazon ile karşılaştırmayı amaçladık. Gereç ve Yöntem: Yerel etik komitenin onayından sonra, 40 Sprague-Dawley erkek sıçan randomize olarak kontrol grubu (n=8), lipopolisakkarit grubu (n=8), astaksantin grubu (n=8), astaksantin + lipopolisakkarit grubu (n=8) ve deksametazon + lipopolisakkarit grubu (n=8) olarak belirlendi. İlk gün bu gruplara sırasıyla dimetil sülfoksit, Salmonella typhimurium lipopolisakkariti, dimetil sülfoksit içinde çözünmüş astaksantin, astaksantin ve lipopolisakkarit ve deksametazon verildi. Yirmi dördüncü saatin sonunda sıçanlara laparotomi yapılarak karaciğer ve kan örnekleri alındı. İstatistiksel analiz için GraphPad Prism6 programı kullanıldı. 0,05’ten küçük p değerleri anlamlı kabul edildi. Bulgular: Lipopolisakkarit grubu ile karşılaştırıldığında, her iki tedavi grubunda da nükleer faktörkappa B seviyelerinde istatistiksel olarak anlamlı bir düşüş vardı. Tedavi gruplarında lipopolisakkarit grubuna göre apoptotik hücreler ve reaksiyon şiddeti anlamlı olarak azaldı. Sonuç: Astaksantin kullanımının, karaciğer dokusunda sepsis tedavisi konusunda olumlu bir etkisi olduğunu bulduk. Farklılıklar olmasına rağmen, deksametazon uygulamasına kıyasla benzer ölçüm değerleri elde edildi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Çobaner N, YELKEN B, Erkasap N, OZKURT M, Bektur E (2022). Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. , 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| Chicago | Çobaner Nurdan,YELKEN BIRGUL BUYUKKIDAN,Erkasap Nilufer,OZKURT METE,Bektur Ezgi Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. (2022): 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| MLA | Çobaner Nurdan,YELKEN BIRGUL BUYUKKIDAN,Erkasap Nilufer,OZKURT METE,Bektur Ezgi Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. , 2022, ss.79 - 85. 10.4274/tybd.galenos.2021.52523 |
| AMA | Çobaner N,YELKEN B,Erkasap N,OZKURT M,Bektur E Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. . 2022; 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| Vancouver | Çobaner N,YELKEN B,Erkasap N,OZKURT M,Bektur E Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. . 2022; 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| IEEE | Çobaner N,YELKEN B,Erkasap N,OZKURT M,Bektur E "Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats." , ss.79 - 85, 2022. 10.4274/tybd.galenos.2021.52523 |
| ISNAD | Çobaner, Nurdan vd. "Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats". (2022), 79-85. https://doi.org/10.4274/tybd.galenos.2021.52523 |
| APA | Çobaner N, YELKEN B, Erkasap N, OZKURT M, Bektur E (2022). Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. Türk Yoğun Bakım Dergisi, 20(2), 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| Chicago | Çobaner Nurdan,YELKEN BIRGUL BUYUKKIDAN,Erkasap Nilufer,OZKURT METE,Bektur Ezgi Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. Türk Yoğun Bakım Dergisi 20, no.2 (2022): 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| MLA | Çobaner Nurdan,YELKEN BIRGUL BUYUKKIDAN,Erkasap Nilufer,OZKURT METE,Bektur Ezgi Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. Türk Yoğun Bakım Dergisi, vol.20, no.2, 2022, ss.79 - 85. 10.4274/tybd.galenos.2021.52523 |
| AMA | Çobaner N,YELKEN B,Erkasap N,OZKURT M,Bektur E Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. Türk Yoğun Bakım Dergisi. 2022; 20(2): 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| Vancouver | Çobaner N,YELKEN B,Erkasap N,OZKURT M,Bektur E Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats. Türk Yoğun Bakım Dergisi. 2022; 20(2): 79 - 85. 10.4274/tybd.galenos.2021.52523 |
| IEEE | Çobaner N,YELKEN B,Erkasap N,OZKURT M,Bektur E "Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats." Türk Yoğun Bakım Dergisi, 20, ss.79 - 85, 2022. 10.4274/tybd.galenos.2021.52523 |
| ISNAD | Çobaner, Nurdan vd. "Investigation of the Anti-inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharideinduced Sepsis in Rats". Türk Yoğun Bakım Dergisi 20/2 (2022), 79-85. https://doi.org/10.4274/tybd.galenos.2021.52523 |
