TY  - JOUR
TI  - Serum Expression Levels of Certain miRNAs in Predicting Diagnosis,  Prognosis, and Response to Chemotherapy in Malignant Pleural  Mesothelioma
AB  - Background: miRNAs are involved in tumor pathogenesis and can  therefore be determined in the primary tumor, plasma and serum, and  body fluids. As in various cancers, their role in the diagnosis, prognosis,  and treatment of patients with malignant pleural mesothelioma (MPM)  may be important.  Aims: To analyze the predictive value of miR-16-5p, miR-29c-3p,  miR-31-5p, miR-125a-5p, miR-320a, miR-484 and miR-532-5p  expressions for diagnosis, prognosis and response to treatment in  patients with MPM. Study Design: Prospective case-control study. Methods: In the first phase of the study, blood samples were collected  from 101 MPM patients before chemotherapy and from 24 healthy  donors (HDs). In the second phase, the blood samples were collected  from 74 MPM patients who had received chemotherapy when the  best overall response and disease recurrence were determined. A  quantitative real-time polymerase chain reaction was undertaken to  detect the miRNA expression levels. The miRNA expression profiles  of MPM patients were compared with those of HDs. The associations  between the expression levels of miRNAs and prognosis and response  to treatment were then evaluated. Results: All miRNAs, except miR-31-5p, were expressed differently  in MPM relative to that in HDs. The expression level of miR-16- 5p decreased when compared with that of HDs, and the expression  levels of miR-29c-3p, miR-125a-5p, miR-320a, miR-484, and miR-532-5p increased when compared with that of HDs. The sensitivity  and specificity values of miR-29c-3p, miR-125a-5p, miR-320a,  miR-484, and miR-532-5p for discriminating MPM from HDs were  85.9% and 59.1%, 95.1% and 62.5%, 87.1% and 79.2%, 82.2% and  58.3%, and 69.3% and 82.6%, respectively. After adjusting for the  histological subtype, stage, and treatment, the miR-29c-3p, miR-125a-5p, and miR-484 were associated with longer survival. The miRNA  expression levels did not change longitudinally for the determination  of chemotherapy response and recurrence. Conclusion: miRNAs may be useful in diagnosing patients with  MPM and provides helpful information in determining the prognosis  of patients.
AU  - Metintas, Muzaffer
AU  - Ak, Guntulu
AU  - Ozbayer, Cansu
AU  - bogar, filiz
AU  - Metintas, Selma
DO  - 10.4274/balkanmedj.galenos.2022.2022-3-26
PY  - 2022
JO  - Balkan Medical Journal
VL  - 39
IS  - 4
SN  - 2146-3123
SP  - 246
EP  - 254
DB  - TRDizin
UR  - http://search/yayin/detay/1132395
ER  -