Yıl: 2011 Cilt: 35 Sayı: 2 Sayfa Aralığı: 203 - 210 Metin Dili: Türkçe

Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae)

Öz:
Endemik bir tür olan Astragalus chrysochlorus Boiss. & Kotschy için etkin bir mikroçoğaltım ve kök kültürü protokolü geliştirilmiştir. En yüksek gövde gelişim frekansı (%100) ve maksimum gövde sayısı (13 gövde/hipokotil eksplantı), 0.5 mg/L trans-Zeatin riboside içeren Murashige ve Skoog (MS) besiyerinde sağlanmıştır. Geliştirilen gövdecikler %2 sukroz içeren MS besiyerinde %93 oranında köklendirilmiştir. Hipokotil explantından yüksek frekansta kallus oluşumunun indüklenmesi ve gelişimi 0.5 mg/L 2,4-Dichlorophenoxyacetic acid içeren MS besiyerinde elde edilmiştir. İndirekt organogenez yoluyla kallustan bitki rejenerasyonu 0.5 mg/L ZR içeren MS besiyerinde gerçekleştirilmiştir. Kök kültürü gelişimi ise 0.5 mg/L α-Naphthaleneacetic acid içeren sıvı MS besiyerinde elde edilmiştir. Bu çalışma ile geliştirilen ve optimize edilen in vitro mikroçoğaltım, kallus ve kök kültürü protokolleri, Astragalus L. türlerinin vejetatif çoğaltımına ve sekonder metabolit çalışmaları için hücre/kök kültürlerinin kullanımına olanak sağlayacaktır.
Anahtar Kelime: gençleştirme bitki büyüme düzenleyicileri Astragalus kallus kaynakların korunması endemik bitkiler Türkiye iklime uyum koruma Astragalus chrysochlorus organ oluşumu

Türkiye endemiklerinden Astragalus chrysochlorus (Leguminosae) türünün mikroçoğaltımı ve kök kültürleri

Öz:
Effi cient micropropagation and root culture protocols were developed for the endemic Astragalus chrysochlorus Boiss. & Kotschy. A high frequency of shoot formation (100%) and maximum multiplication (13 shoots per hypocotyl explant) were achieved on Murashige and Skoog (MS) media supplemented with 0.5 mg/L trans-Zeatin riboside (ZR). By using single regenerated hypocotyl explants, rooting was achieved at a rate of 93% on MS medium containing 2% sucrose without growth regulators. High frequency callus initiation and growth were achieved when single hypocotyl explants were inoculated on MS medium supplemented with 0.5 mg/L 2,4-Dichlorophenoxyacetic acid. Plant regeneration through indirect organogenesis was achieved on MS medium supplemented with 0.5 mg/L ZR. Root cultures were successfully established in liquid MS medium containing 0.5 mg/L α-Naphthaleneacetic acid. Th e optimised in vitro propagation, callus culture, and root culture protocols off er the possibility to use cell/root culture techniques for vegetative propagation and secondary metabolism studies on Astragalus L. species.
Anahtar Kelime: conservation Astragalus chrysochlorus organogenesis plant growth regulators Astragalus regeneration endemic plants resource conservation Turkey acclimatization callus

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA HASANÇEBİ S, TURGUT KARA N, ÇAKIR Ö, ARI Ş (2011). Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). , 203 - 210.
Chicago HASANÇEBİ SEMRA,TURGUT KARA NESLİHAN,ÇAKIR ÖZGÜR,ARI ŞULE Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). (2011): 203 - 210.
MLA HASANÇEBİ SEMRA,TURGUT KARA NESLİHAN,ÇAKIR ÖZGÜR,ARI ŞULE Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). , 2011, ss.203 - 210.
AMA HASANÇEBİ S,TURGUT KARA N,ÇAKIR Ö,ARI Ş Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). . 2011; 203 - 210.
Vancouver HASANÇEBİ S,TURGUT KARA N,ÇAKIR Ö,ARI Ş Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). . 2011; 203 - 210.
IEEE HASANÇEBİ S,TURGUT KARA N,ÇAKIR Ö,ARI Ş "Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae)." , ss.203 - 210, 2011.
ISNAD HASANÇEBİ, SEMRA vd. "Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae)". (2011), 203-210.
APA HASANÇEBİ S, TURGUT KARA N, ÇAKIR Ö, ARI Ş (2011). Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). Turkish Journal of Botany, 35(2), 203 - 210.
Chicago HASANÇEBİ SEMRA,TURGUT KARA NESLİHAN,ÇAKIR ÖZGÜR,ARI ŞULE Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). Turkish Journal of Botany 35, no.2 (2011): 203 - 210.
MLA HASANÇEBİ SEMRA,TURGUT KARA NESLİHAN,ÇAKIR ÖZGÜR,ARI ŞULE Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). Turkish Journal of Botany, vol.35, no.2, 2011, ss.203 - 210.
AMA HASANÇEBİ S,TURGUT KARA N,ÇAKIR Ö,ARI Ş Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). Turkish Journal of Botany. 2011; 35(2): 203 - 210.
Vancouver HASANÇEBİ S,TURGUT KARA N,ÇAKIR Ö,ARI Ş Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae). Turkish Journal of Botany. 2011; 35(2): 203 - 210.
IEEE HASANÇEBİ S,TURGUT KARA N,ÇAKIR Ö,ARI Ş "Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae)." Turkish Journal of Botany, 35, ss.203 - 210, 2011.
ISNAD HASANÇEBİ, SEMRA vd. "Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae)". Turkish Journal of Botany 35/2 (2011), 203-210.