Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research

KORKMAZ BAYRAM, KEZİBAN; Dundar, Munis; Bayram, Arslan; KIRNAP, MEHMET; Kurt Colak, Fatma

doi:10.5336/medsci.2021-87249

Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research

Fatma KURT ÇOLAK, (Sütçü İmam Üniversitesi Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Kahramanmaraş, Türkiye)

Arslan BAYRAM, (Tıbbi Genetik Kliniği, Etlik Zübeyde Hanım Kadın Hastalıkları Eğitim ve Araştırma Hastanesi, Ankara, Türkiye)

Keziban KORKMAZ BAYRAM, (Tıbbi Genetik Anabilim Dalı, Ankara Yıldırım Beyazıt Üniversitesi Tıp Fakültesi, Ankara, Türkiye)

Mehmet KIRNAP, (Erciyes Üniversitesi Tıp Fakültesi, Fizik Tedavi ve Rehabilitasyon Anabilim Dalı, Kayseri, Türkiye)

Münis DÜNDAR (Tıbbi Genetik Anabilim Dalı, Erciyes Üniversitesi Tıp Fakültesi, Kayseri, Türkiye)

Türkiye Klinikleri Tıp Bilimleri Dergisi

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Yıl: 2022 Cilt: 42 Sayı: 3 Sayfa Aralığı: 213 - 220 Metin Dili: İngilizce DOI: 10.5336/medsci.2021-87249 İndeks Tarihi: 27-10-2022

Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research

Öz:

Objective: In this study, the relationship between theMDR1 gene and ankylosing spondylitis (AS) was investigated. Genetic and envi- ronmental factors are known to play an important role in thepathogenesis of AS. It is obvious that different patients have different reactions to prescribed drugs and drug dosages during standard treatment. One of these factors is the P-glycoprotein encoded by the MDR1 gene. The most widely studied alleles of this gene are rs1045642-NM_000927.4:c.3435T>C, p. Ile1145Ile (C3435T), rs2032582-NM_000927.4:c.2677T>G/A, p. Ser893Ala/Thr (G2677T/A) and silent rs1128503-NM_000927.4:c.1236T>C, p. Gly412Gly (C1236T).Mate- rial and Methods: The study group was formed from 34 patients with bio- logical therapy, 32 patients with other treatments (66 AS patients in total) and 32 healthy individuals. DNA isolation was performed using a High Pure PCR Template Preparation Kit. RNA isolation was performed with TRIzol man- ual isolation. For quantitative real-time polymerase chain reaction, a Roche LightCycler FastStart DNA Master device HybProbe was used. Results: There was no statistically significant difference between groups regarding the investigated polymorphisms. In the biological therapy [tumor necrosis factor alpha (TNF-α) inhibitor] group, a significant decrease was found in MDR1 gene expression (p<0.001). Conclusion: In conclusion, we could say that there is no relation between single-nucleotide polymorphism and treatment choice and response for AS patients. Decreased MDR1 expres- sion can be explained by the possible downregulatory effect of TNF-α in- hibitors.

Anahtar Kelime:

MDR1 Geni ile Ankilozan Spondilit Arasındaki İlişkinin İncelenmesi: Olgu Kontrol Araştırması

Öz:

Bu çalışmada MDR1 geni ile Ankilozan spondilit araştırıldı. Genetik ve çevresel faktörlerin ankilozan spondilit (AS) patogenezinde önemli rol oynadığı bilinmektedir. Standart tedavi sırasında farklı hastala- rın, reçeteli ilaçlara ve ilaç dozajlarına karşı farklı tepkileri olduğu açıktır. Bu faktörlerden biri, MDR1 geni tarafından kodlanan P-glikoproteindir. Bu genin en çok çalışılan allelleri rs1045642-NM_0009 27.4:c.3435T>C, p. Ile1145Ile (C3435T), rs2032582- NM_ 000927. 4:c.2677T>G/A, p. Ser893Ala/Thr (G2677T/A) ve sessiz rs1128503-NM_000927.4:c.1236T>C, p. Gly412Gly (C1236T)’dir. Gereç ve Yöntemler: Otuz dört biyolojik tedavi, 32 di ğer te- davileri alan toplam 66 AS hastas ı ve 32 sağlıklı herhangi bir ilaç kullanma- yan bireyden olu şan grup kar şılaştırıldı. DNA izolasyonu High Pure PCR Template Preparation Kiti kullanılarak gerçekleştirildi. RNA izolasyonu tri- zolle yapıldı. Kantitatif gerçek zamanlı polimeraz zincir reaksiyonu, Roche LightCycler FastStart DNA Master HybProbe cihaz ında gerçekleştirildi. Bulgular: Çalışmamızın sonucunda, ara ştırılan polimorfizmlerle ilgili gruplar arasında istatistiksel olarak anlaml ı bir fark yoktu. Biyolojik te- davileri [tümör nekrozis faktör alfa (TNF- α)] kullanan AS grubunun, MDR1 gen ekspresyonu arasında anlamlı bir farklılık bulundu (p<0,001). Sonuç: Sonuç olarak AS hastalarında tek nükleotid polimorfizmi ile tedavi seçimi ve yanıt arasında bir ilişki olmadığını söyleyebiliriz. Azalan MDR1 ekspresyonu, TNF-α inhibitörlerinin olası baskılayıcı regülatör etkisi ile açıklanabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Kurt Colak F, Bayram A, KORKMAZ BAYRAM K, KIRNAP M, Dundar M (2022). Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. , 213 - 220. 10.5336/medsci.2021-87249
Chicago	Kurt Colak Fatma,Bayram Arslan,KORKMAZ BAYRAM KEZİBAN,KIRNAP MEHMET,Dundar Munis Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. (2022): 213 - 220. 10.5336/medsci.2021-87249
MLA	Kurt Colak Fatma,Bayram Arslan,KORKMAZ BAYRAM KEZİBAN,KIRNAP MEHMET,Dundar Munis Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. , 2022, ss.213 - 220. 10.5336/medsci.2021-87249
AMA	Kurt Colak F,Bayram A,KORKMAZ BAYRAM K,KIRNAP M,Dundar M Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. . 2022; 213 - 220. 10.5336/medsci.2021-87249
Vancouver	Kurt Colak F,Bayram A,KORKMAZ BAYRAM K,KIRNAP M,Dundar M Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. . 2022; 213 - 220. 10.5336/medsci.2021-87249
IEEE	Kurt Colak F,Bayram A,KORKMAZ BAYRAM K,KIRNAP M,Dundar M "Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research." , ss.213 - 220, 2022. 10.5336/medsci.2021-87249
ISNAD	Kurt Colak, Fatma vd. "Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research". (2022), 213-220. https://doi.org/10.5336/medsci.2021-87249

APA	Kurt Colak F, Bayram A, KORKMAZ BAYRAM K, KIRNAP M, Dundar M (2022). Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. Türkiye Klinikleri Tıp Bilimleri Dergisi, 42(3), 213 - 220. 10.5336/medsci.2021-87249
Chicago	Kurt Colak Fatma,Bayram Arslan,KORKMAZ BAYRAM KEZİBAN,KIRNAP MEHMET,Dundar Munis Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. Türkiye Klinikleri Tıp Bilimleri Dergisi 42, no.3 (2022): 213 - 220. 10.5336/medsci.2021-87249
MLA	Kurt Colak Fatma,Bayram Arslan,KORKMAZ BAYRAM KEZİBAN,KIRNAP MEHMET,Dundar Munis Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. Türkiye Klinikleri Tıp Bilimleri Dergisi, vol.42, no.3, 2022, ss.213 - 220. 10.5336/medsci.2021-87249
AMA	Kurt Colak F,Bayram A,KORKMAZ BAYRAM K,KIRNAP M,Dundar M Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. Türkiye Klinikleri Tıp Bilimleri Dergisi. 2022; 42(3): 213 - 220. 10.5336/medsci.2021-87249
Vancouver	Kurt Colak F,Bayram A,KORKMAZ BAYRAM K,KIRNAP M,Dundar M Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research. Türkiye Klinikleri Tıp Bilimleri Dergisi. 2022; 42(3): 213 - 220. 10.5336/medsci.2021-87249
IEEE	Kurt Colak F,Bayram A,KORKMAZ BAYRAM K,KIRNAP M,Dundar M "Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research." Türkiye Klinikleri Tıp Bilimleri Dergisi, 42, ss.213 - 220, 2022. 10.5336/medsci.2021-87249
ISNAD	Kurt Colak, Fatma vd. "Investigation of Relation Between MDR1 Gene and Ankylosing Spondylitis: Case Control Research". Türkiye Klinikleri Tıp Bilimleri Dergisi 42/3 (2022), 213-220. https://doi.org/10.5336/medsci.2021-87249