INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS

BOZKURT, N.Neslihan; Hürkul, Muhammed Mesud; Yayla, Şeyda; Aysal, Ayhan ibrahim; Tok, Kenan Can

doi:10.33483/jfpau.1133615

INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS

Kenan Can TOK, (Ankara Üniversitesi, Adli Bilimler Enstitüsü, Adli Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Muhammed Mesud HÜRKUL, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Botanik Anabilim Dalı, Ankara, Türkiye)

N.Neslihan BOZKURT, (Ankara Üniversitesi, Adli Bilimler Enstitüsü, Adli Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Ayhan İbrahim AYSAL, (Gazi Üniversitesi, Eczacılık Fakültesi, Farmasötik Kimya Anabilim Dalı, Ankara, Türkiye)

Şeyda YAYLA (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Botanik Anabilim Dalı, Ankara, Türkiye)

Ankara Üniversitesi Eczacılık Fakültesi Dergisi

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Yıl: 2022 Cilt: 46 Sayı: 3 Sayfa Aralığı: 827 - 838 Metin Dili: İngilizce DOI: 10.33483/jfpau.1133615 İndeks Tarihi: 27-10-2022

INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS

Öz:

Objective: Silene L. (Caryophyllaceae) species are traditionally used for the treatment of inflammation, urinary inflammation, eye trouble, skin problem, stomach ache, dysentery, tooth decay, fever, headache, malaria, pimples and backache. Chemical ingredients of Silene species consist of flavonoids, anthocyanidins, terpenoids, triterpene saponins, phytoecdysteroids, benzenoids, vitamins, and show antioxidant, anti- inflammatory, antitumor and antiviral activities. Silene ruscifolia (Hub.-Mor. & Reese) Hub.-Mor. is called "gizli nakıl" in Türkiye. Material and Method: The plant material was collected from Beynam Forest (Ankara/Türkiye). The aerial parts of the plant were extracted with methanol in an ultrasonic bath. HPLC system (Agilent 1260 Series) with an autosampler, binary pump, column oven, and a UV detector was coupled with an iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) with a Dual Spray Agilent Jet Stream electrospray ionization source. Agilent TC C-18 (4.6 mm x 150 mm x 5 μm) column was used for the separation of the compounds. The GC-MS analysis of extract was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used to determine and identify the compounds. Result and Discussion: LC-MS Q-TOF analysis showed that S. ruscifolia contained rutin, narcissin, luteolin, isorhamnetin, rhamnetin and quercetin dimethyl ether. GC-MS analysis showed that the extract had the highest content of sugar (50.5%) and sugar alcohols (46.39%). It also contains carboxylic acid (0.47%), fatty acid (0.64%), sugar acid (0.42%), glycoside (0.48%), carotenoids (0.61%) and benzoic acid ester (0.49%). D-pinitol has the highest content in the extract with 41.14%.

Anahtar Kelime:

SILENE RUSCIFOLIA METANOLİK HERBA EKSTRESİNDEKİ FİTOKİMYASALLARIN LC- QTOF/MS VE GC/MS İLE İNCELENMESİ

Öz:

Amaç: Silene L. (Caryophyllaceae) türleri geleneksel olarak iltihap, idrar yolu iltihabı, göz rahatsızlığı, cilt sorunu, mide ağrısı, dizanteri, diş çürümesi, ateş, baş ağrısı, sıtma, sivilce ve sırt ağrısı tedavisinde kullanılmaktadır. Silene türlerinin kimyasal bileşenleri flavonoidler, antosiyanidinler, terpenoidler, triterpen saponinler, fitoekdisteroidler, benzenoidler, vitaminlerden oluşur ve antioksidan, antiinflamatuar, antitümör, antiviral aktivite gösterirler. Silene ruscifolia (Hub.-Mor. & Reese) Hub.-Mor. Türkiye'de "gizli nakıl" olarak adlandırılır. Gereç ve Yöntem: Bitki materyali Beynam Ormanı'ndan (Ankara/Türkiye) toplandı. Bitkinin toprak üstü kısımları ultrasonik banyoda metanol ile ekstre edildi. Otomatik örnekleyici, ikili pompa, kolon fırını ve bir UV dedektörüne sahip HPLC sistemi (Agilent 1260 Serisi), Çift Sprey Agilent Jet Stream elektrosprey iyonizasyon kaynağına sahip bir iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) ile birleştirildi. Bileşiklerin ayrılması için Agilent TC C-18 (4.6 mm x 150 mm x 5 μm) kolonu kullanıldı. Ekstrenin GC-MS analizi, bir Agilent 5973N dört kutuplu kütle spektrometresi (Agilent, ABD) ile donatılmış bir Agilent 6890 gaz kromatografı kullanılarak yapıldı. Bileşiklerin belirlenmesi ve tanımlanması için Mass Hunter yazılımı (Qualitative Analysis B.07.00) ve NIST Mass Spectral Library (2014) kullanıldı. Sonuç ve Tartışma: LC-MS Q-TOF analizi, S. ruscifolia'nın rutin, narsissin, luteolin, izoramnetin, ramnetin ve kersetin dimetil eter içerdiğini gösterdi. GC-MS analizi, ekstrenin şeker (%50.5) ve şeker alkolleri (%46.39) olarak en yüksek içeriğe sahip olduğunu göstermiştir. Ayrıca karboksilik asit (%0.47), yağ asidi (%0.64), şeker asidi (%0.42), glikozit (%0.48), karotenoidler (%0.61) ve benzoik asit esteri (%0.49) içerir. D- pinitol, %41.14 ile ekstredeki en yüksek içeriğe sahiptir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Tok K, Hürkul M, BOZKURT N, Aysal A, Yayla Ş (2022). INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. , 827 - 838. 10.33483/jfpau.1133615
Chicago	Tok Kenan Can,Hürkul Muhammed Mesud,BOZKURT N.Neslihan,Aysal Ayhan ibrahim,Yayla Şeyda INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. (2022): 827 - 838. 10.33483/jfpau.1133615
MLA	Tok Kenan Can,Hürkul Muhammed Mesud,BOZKURT N.Neslihan,Aysal Ayhan ibrahim,Yayla Şeyda INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. , 2022, ss.827 - 838. 10.33483/jfpau.1133615
AMA	Tok K,Hürkul M,BOZKURT N,Aysal A,Yayla Ş INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. . 2022; 827 - 838. 10.33483/jfpau.1133615
Vancouver	Tok K,Hürkul M,BOZKURT N,Aysal A,Yayla Ş INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. . 2022; 827 - 838. 10.33483/jfpau.1133615
IEEE	Tok K,Hürkul M,BOZKURT N,Aysal A,Yayla Ş "INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS." , ss.827 - 838, 2022. 10.33483/jfpau.1133615
ISNAD	Tok, Kenan Can vd. "INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS". (2022), 827-838. https://doi.org/10.33483/jfpau.1133615

APA	Tok K, Hürkul M, BOZKURT N, Aysal A, Yayla Ş (2022). INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 46(3), 827 - 838. 10.33483/jfpau.1133615
Chicago	Tok Kenan Can,Hürkul Muhammed Mesud,BOZKURT N.Neslihan,Aysal Ayhan ibrahim,Yayla Şeyda INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi 46, no.3 (2022): 827 - 838. 10.33483/jfpau.1133615
MLA	Tok Kenan Can,Hürkul Muhammed Mesud,BOZKURT N.Neslihan,Aysal Ayhan ibrahim,Yayla Şeyda INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, vol.46, no.3, 2022, ss.827 - 838. 10.33483/jfpau.1133615
AMA	Tok K,Hürkul M,BOZKURT N,Aysal A,Yayla Ş INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2022; 46(3): 827 - 838. 10.33483/jfpau.1133615
Vancouver	Tok K,Hürkul M,BOZKURT N,Aysal A,Yayla Ş INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2022; 46(3): 827 - 838. 10.33483/jfpau.1133615
IEEE	Tok K,Hürkul M,BOZKURT N,Aysal A,Yayla Ş "INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS." Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 46, ss.827 - 838, 2022. 10.33483/jfpau.1133615
ISNAD	Tok, Kenan Can vd. "INVESTIGATION OF PHYTOCHEMICALS IN METHANOLIC HERBA EXTRACT OF SILENE RUSCIFOLIA BY LC-QTOF/MS AND GC/MS". Ankara Üniversitesi Eczacılık Fakültesi Dergisi 46/3 (2022), 827-838. https://doi.org/10.33483/jfpau.1133615