PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY
Yıl: 2022 Cilt: 46 Sayı: 3 Sayfa Aralığı: 872 - 882 Metin Dili: İngilizce DOI: 10.33483/jfpau.1134347 İndeks Tarihi: 27-10-2022
PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY
Öz: Objective: The aim of this research was to assess the effect of the process and formulation parameters during the preparation of solid lipid microparticles. Solid lipid microparticles (SLMs) have evident advantages such as biocompatibility, simplicity of production and characterization, prolonged release, and especially high protein loading capacity, despite being less investigated than lipid nanoparticles. Material and Method: SLMs were prepared via emulsion solvent diffusion technique using glyceryl tridecanoate (GTD) as a biocompatible and biodegradable lipid. The optimum formulation conditions for producing homogenous spherical microparticles were found and represented by a triangle phase diagram area. After optimizing the particle size and encapsulation efficiency by changing the formulation parameters, the microparticles were characterized by in vitro release, morphological analysis, thermal analysis and electrophoretic analysis on the selected formulations. Result and Discussion: The maximum drug loading efficiency was achieved by combining 100 mg of lipid, 60% triacetin and 3% emulsifier. The average microparticle size was observed as 8.9 μm. The in vitro drug release were analyzed in pH 7.4 phosphate buffer and were mainly completed at 8th hour.
Anahtar Kelime: -
Öz: Bu araştırmanın amacı, katı lipid mikropartiküllerinin hazırlanması sırasında proses ve formülasyon parametrelerinin etkisini değerlendirmektir. Katı lipid mikropartiküller (SLM'ler), lipid nanopartiküllerinden daha az araştırılmış olmalarına rağmen biyouyumluluk, üretim ve karakterizasyon kolaylığı, uzun süreli salım ve özellikle yüksek protein yükleme kapasitesi gibi belirgin avantajlara sahiptir. Gereç ve Yöntem: SLM'ler, biyouyumlu ve biyolojik olarak parçalanabilen bir lipid olarak gliseril tridekanoat (GTD) kullanılarak emülsiyon çözücü difüzyon tekniği ile hazırlanmıştır. Homojen küresel mikropartiküller üretmek için en iyi formülasyon koşulları belirlenmiş ve bir üçgen faz diyagram alanı ile temsil edilmiştir. Mikropartiküller, formülasyon parametreleri değiştirilerek partikül boyutu ve enkapsülasyon etkinliği optimize edildikten sonra, seçilen formülasyonlar in vitro salım, morfolojik analizler, termal analiz ve elektroforetik analiz ile karakterize edilmiştir. Sonuç ve Tartışma: En yüksek etken madde yükleme etkinliği 100 mg lipid, %60 triasetin ve %3 emülgatör kullanılarak elde edilmiştir. Ortalama mikropartikül boyutu 8.9 μm olarak gözlenmiştir. İn vitro etken madde salımı pH 7.4 fosfat tampon çözeltisinde değerlendirilmiş ve 8. saatte tamamlanmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Küçüktürkmen B, Oz U, Bozkır A (2022). PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. , 872 - 882. 10.33483/jfpau.1134347 |
| Chicago | Küçüktürkmen Berrin,Oz Umut Can,Bozkır Asuman PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. (2022): 872 - 882. 10.33483/jfpau.1134347 |
| MLA | Küçüktürkmen Berrin,Oz Umut Can,Bozkır Asuman PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. , 2022, ss.872 - 882. 10.33483/jfpau.1134347 |
| AMA | Küçüktürkmen B,Oz U,Bozkır A PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. . 2022; 872 - 882. 10.33483/jfpau.1134347 |
| Vancouver | Küçüktürkmen B,Oz U,Bozkır A PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. . 2022; 872 - 882. 10.33483/jfpau.1134347 |
| IEEE | Küçüktürkmen B,Oz U,Bozkır A "PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY." , ss.872 - 882, 2022. 10.33483/jfpau.1134347 |
| ISNAD | Küçüktürkmen, Berrin vd. "PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY". (2022), 872-882. https://doi.org/10.33483/jfpau.1134347 |
| APA | Küçüktürkmen B, Oz U, Bozkır A (2022). PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 46(3), 872 - 882. 10.33483/jfpau.1134347 |
| Chicago | Küçüktürkmen Berrin,Oz Umut Can,Bozkır Asuman PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi 46, no.3 (2022): 872 - 882. 10.33483/jfpau.1134347 |
| MLA | Küçüktürkmen Berrin,Oz Umut Can,Bozkır Asuman PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, vol.46, no.3, 2022, ss.872 - 882. 10.33483/jfpau.1134347 |
| AMA | Küçüktürkmen B,Oz U,Bozkır A PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2022; 46(3): 872 - 882. 10.33483/jfpau.1134347 |
| Vancouver | Küçüktürkmen B,Oz U,Bozkır A PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2022; 46(3): 872 - 882. 10.33483/jfpau.1134347 |
| IEEE | Küçüktürkmen B,Oz U,Bozkır A "PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY." Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 46, ss.872 - 882, 2022. 10.33483/jfpau.1134347 |
| ISNAD | Küçüktürkmen, Berrin vd. "PREPARATION AND IN VITRO CHARACTERIZATION OF SOLID LIPID MICROPARTICLES FOR PROTEIN DELIVERY". Ankara Üniversitesi Eczacılık Fakültesi Dergisi 46/3 (2022), 872-882. https://doi.org/10.33483/jfpau.1134347 |
