TY  - JOUR
TI  - DETERMINATION OF ALPHA-2-MACROGLUBULIN IN SERUM SAMPLES
AB  - Objective: Proteomics is one of the fastest growing omics that has been extensively used in clinical studies. Proteomics involves qualitative and quantitative protein analysis in a wide range of samples starting from a single cell to complex biological samples. Protein-based biomarker studies have been applied to many diseases including metabolic diseases, cancer and neuropsychiatric diseases for both diagnostic and prognostic purposes. Alpha-2-macroglubulin (A2MG) is a clinically relevant secreted protein involving in various biological processes including blood coagulation, protein binding and protease inhibition. Current methods for A2MG analysis are limited, as they focus on either immune-specific binding through a certain protein unit or a unique peptide. As a single protein could be in different forms (complexes, modifications, etc) and the biological activity is structure specific, an extensive analysis is necessary. Here a new Mass-Spectrometry (MS) based method was developed for comprehensive A2MG analysis. Material and Method: A reference human serum and A2MG protein standard were used for method development. Proteolytic protein digestion was performed using trypsin and Circular-Dichroism (CD) spectroscopy was used to ensure protein unfolding and denaturation prior to digestion. Targeted MS method was developed to monitor 12 unique peptides for A2MG in serum. Result and Discussion: Monitoring multiple peptides for a single protein enabled to observe biological differences offer a robust and reliable A2MG analysis in serum. The method can also easily be implemented to other proteins. The concept of targeted-MS provides an ideal quantification and validation platform which then can be easily transferred to clinical laboratories.
AU  - Ozcan, Sureyya
DO  - 10.33483/jfpau.1139157
PY  - 2022
JO  - Ankara Üniversitesi Eczacılık Fakültesi Dergisi
VL  - 46
IS  - 3
SN  - 1015-3918
SP  - 967
EP  - 978
DB  - TRDizin
UR  - http://search/yayin/detay/1135394
ER  -