TY  - JOUR
TI  - Protective Role of Progesterone on Lung Injury Induced by Ischemia Reperfusion of the Lower Limbs
AB  - Objective: Remote lung injury is one of the most challenging issues in patients undergoing  ischemia reperfusion (IR) injury of the lower limbs. We examined the role of progesterone  (PG) on the remote injury of the lungs seen after IR of the lower limbs. Methods: Eighteen male Sprague Dawley rats were divided into three groups. (1) Con trol: rats had only two physiological saline injections intraperitoneally (i.p.) 2 h apart under  general anesthesia. (2) IR: underwent 2 h tourniquet induced ischemia for both lower limbs  followed by 2 h of reperfusion. Animals were injected with physiological saline (i.p.) before  both ischemia and reperfusion. (3) IR + PG: rats underwent the same IR protocol as the  IR group and were injected with PG (16 mg/kg, i.p.) before both ischemia and reperfusion.  After reperfusion, rats were sacrificed, and lung tissues were taken out for histopathologic  and biochemical analyses. Results: In IR + PG group, tissue levels of malondialdehyde and nitric oxide decreased  significantly compared with the IR group (p<0.01). Similarly, glutathione level and superox ide dismutase and glutathione S-transferase activities significantly increased in the IR + PG  group than in the IR group (p<0.05, p<0.01, and p<0.05, respectively). In light microscopy,  reduced inflammatory cell infiltration, amelioration in alveolar structure, and mild vascular  congestion in the parenchyma were seen in the IR + PG group. A significant improvement in  histopathologic score was seen in the IR + PG group compared with the IR group (p<0.001).
AU  - ak, koray
AU  - Özbeyli, Dilek
AU  - Ak, Esin
AU  - Oktay, Sehkar
AU  - Muhan, Aleyna
DO  - 10.14744/scie.2022.57873
PY  - 2022
JO  - Southern Clinics of Istanbul Eurasia
VL  - 33
IS  - 3
SN  - 2587-0998
SP  - 320
EP  - 325
DB  - TRDizin
UR  - http://search/yayin/detay/1135420
ER  -