Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation

Hayat Topçu (Tarımsal Biyoteknoloji Bölümü, Tekirdağ Namık Kemal Üniversitesi, Tekirdağ, Türkiye)
Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online)
8 0
Yıl: 2022 Cilt: 6 Sayı: 3 Sayfa Aralığı: 205 - 217 Metin Dili: İngilizce DOI: 10.29329/ijiaar.2022.475.4 İndeks Tarihi: 28-10-2022

Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation

Öz:
Rubus spp. is a shrub-form plant known for its fruits called blackberries. Blackberries are plants with high commercial value, delicious taste, nice aroma, and high nutritional value. Turkey has wealthy genetic origins of Rubus species. Conventionally, the trading propagation of Rubus plants is done as vegetatively, utilizing truncation, rooting, or stratuming. However, these traditional methods are time-consuming and inefficient in virus-free plant production. Cloning of plant grown in the tissue culture also enables to obtain virus-free plants and to provide fast replicating high standard plants. Rubus obtained by micropropagation is used for the formation of commercial fruit plantations as well as source plant formation. In this work, the aim is the development of in vitro micropropagation process of the wild Rubus in the Trakya Region. Proliferation from axillary buds was made by adding BAP (6- Benzylaminopurine), NAA (Naphthalinacetic acid) and GA3 (Gibberellic acid) in various combinations and concentrations to the MS medium. Rooting was successfully realized with 83.3% rooted plants in 1 IBA medium. No roots were seen in 0 MS. The survival rate of plants transferred to ex vitro conditions was 100%.
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  • Aguilera-Arango, G.A., Gómez-López, E.D. & González-Mejia. A. (2019). Callogénesisen cultivares híbridos de Cocos nucifera L. mediante cultivo in vitro de inflorescencias in maduras. Biotecnología Vegeta, l 19(4):277-284.
  • Akin, M., Eyduran, S.P., Ercisli, S., Toteva, V.K. & Eyduran, E. (2016). Phytochemical profiles of wild blackberries, black and white mulberries fr.om southern Bulgaria. Biotechnology & Biotechnological Equipment, 30:899-906.
  • Andrade, A., Gómez, L., Torres, Y. & Aguilera-Arango., G. (2021). Evaluation Of Growing Media for the In Vitro Establishment, Multiplication and Rooting of Blackberry (Rubus glaucusBenth.). Chilean J. Agric. Anim. Sci., ex Agro-Ciencia, 37(2):117-127.
  • Bite, A. & Petrevica, L. (2002). The influence of in vitro propagation on the field behaviour of red raspberry variety ‘Norna’. Acta Hortic., 585, 615–619.
  • Bobrowski, V. L., Mello-Farias, P. & Peters, C.P. (1996). Micropropagation of blackberries (Rubus sp.) cultivars. Revista Brasileira de Agrociencia 2:17-20 (in Italian).
  • Borodulina, I.D., Plaksina, T.V., Panasenko, V.N. & Sokolova, G.G. (2019). Оptimization of blackberry clonal micropropagation. Ukrainian Journal of Ecology, 9(3):339-345.
  • Boxus, P., Damiano, C. & Brasseur, E. (1989). Strawberry. In: Ammirato P, Evans d, Sharp W, Yamada Y (Eds). Handbook of plant cell culture. New York, Macmillan pp 453-486.
  • Broome, O.C. & Zimmerman, R.H. (1978). Invitro propagation of blackberry. HortScience, 13:151-153.
  • Caldwell, J.D. (1984). Blackberry propagation. HortScience, 2:193-195.
  • Cancino-Escalante, G.O., Sánchez-Montaño, L.R., Quevedo-García, E. & Díaz-Carvajal, C. (2011).
  • Caracterización fenotípica de accesiones de especies de Rubus L. de los municipios de Pamplona y Chitagá, región Nororiental de Colombia. Universitas Scientiarum 16(3):219-233.
  • Cao, X &, Hammerschlag, F.A. (2000). Improved shoot organogenesis from leaf explants of highbush blueberry. HortScience, 35:945-947.
  • Cousineau, J.C. & Donnelly, D.J. (1991). Adventitious shoot regeneration from leaf explants of tissue cultured and greenhouse-grown raspberry. Plant Cell Tissue Organ Culture, 27:249-255.
  • De Oliveira, R.P. & Nino, A.F.P. (2009). In vıtro multiplication rate of raspberry cultıvars. Rev. Bras. Frutic., Jaboticabal - SP, 31,1, 280-284 (in Portuguese).
  • Debnath, S.C. (2004). Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation. AGRIS. 43(2):179-186. ISSN: 0167-6903.
  • Demenko, V.I., Shestibratov, K.A. & Lebedev, V.G. (2014). Ukorenenie – klyuchevoj etap razmnozheniya rastenij in vitro. Izvestiya TSKHA, 1, 13–26 (in Russian).
  • Diaconeasa, Z., Ranga, F., Rugină, D., Cuibus, L. & Socaciu, C. (2014). HPLC/PDA-ESI/MS identification of phenolic acids, flavonol glycosides and antioxidant potential in blueberry, blackberry, raspberries and cranberries. Journal of Food and Nutrition Research, 2:781-785.
  • Dziedzic, E. & Jagła, J. (2013). Micropropagation of Rubus and Ribes spp. In Protocols for Micropropagation of Selected Economically Important Horticultural Plants; Lambardi, M., Ozudogru, E.A., Jain, S.M., Eds.; Humana Press: Totowa, NJ, USA, 149–160. ISBN 978-1-62703-073-1.
  • Ercisli, S. (2014). A short review of the fruit germplasm resources of Turkey. Genetic Resources and Crop Evaluation, 51, 419-435.
  • Ercisli, S. & Orhan, E. (2005). Natural mulberry (Morus spp.) production in Erzurum region in Turkey. In Proceedings of the international scientific conference, ‘environmentally friendly fruit growing’ (p. 129–136). 7–9 September 2005, Tartu – Estonia.
  • Espinosa, B.N., M.G.A., Ligarreto, M.L.S., Barrero, C.C.I. & Medina. (2016). Variabilidad morfológica de variedades nativas de mora (Rubus sp.) en los Andes de Colombia. Revista Colombiana de Ciencias Hortícolas, 10(2):211-221.
  • Fiola, J.A., Hassan, M.A., Swartz, H.J. & McNicols, R. (1990). Effect of thidiazuron, light fluence rates and kanamycin on in vitroshoot organogenesis from excised Rubus cotyledons and leaves. Plant Cell Tissue Organ Culture, 20:223-228.
  • Galletta, G.J., Draper, A.D., Maas, J.L., Skirvin, R.M., Otterbacher, A.G., Swartz, H.J. & Chandler C.K. (1998). Chester thornless, blackberry, Fruit Var. J., 52(3), 118-122.
  • Georgieva, M., Kondakova, V., Dragoyski, K., Georgiev, D. & Naydenova, G. (2009). Comparative study of raspberry cv. Balgarski Rubin propagated by classical and in vitro methods. J. Pomol. 43, 81–86.
  • Graham, J., Squire, G.R., Marshall, B. & Harrison, R.E. (1997). Spatially-dependent genetic diversity within and between colonies of wild raspberry Rubus idaeus detected using RAPD markers. Mol Ecol 6: 272– 281.
  • Huang, J.Y. & Hu, J. M. (2009). Revision of Rubus (Rosaceae) in Taiwan. Taiwania, 54(4):285-310.
  • Jadan, M., Ruiz, J., Soria, N. & Mihal, R.A. (2015). Synthetic seeds production and the induction of organogenesis in blackberry (Rubus glaucusBenth). Romanian Biotechnological Letters, 20:10134- 10142.
  • Kefayeti, S., Kafkas, E.& Ercisli, S. (2019). Micropropagation of ʻChesterthornlessʼ Blackberry Cultivar using Axillary Bud Explants. Not. Bot. HortiAgrobo, 47(1), 162–168.
  • Leitzke, L., Damiani C. & Wulff, M. (2009). Multiplicação e enraizamento in vitro de amoreira-preta “Xavante”: efeito da concentração de sais, do tipo de explante e de carvão ativado no meio de cultura, Ciência agrotecnologia. Lavras, 33, 1959-1966.
  • Martin, R.R. (2002). Virus diseases of Rubus and strategies for their control. Acta Hortic. 585, 265–270.
  • Marulanda, M., Carvajalino, M. & Vento, H. (2000). Establecimiento y multiplicación in vitro de plantas seleccionadas de Rubus glaucus Benth para el departamento de Risaralda (Colombia). Actualidades Biológicas, 22(73), 121-129.
  • Matushkin, S. A.& Yarmolenko, L. V. (2017). Vliyanie mineral'nogo sostava pitatel'noj sredy na rizogenez yagodnyh kul'tur in vitro. Sbornik nauchnyh trudov GNBS, t. 144, 2, 73–76 (in Russian).
  • Meng, R., Chen, T.H.H., Finn, C. E. & Li, J. (2004). Improving in vitro plant regeneration from leaf and petiole explants of ‘Marion’ blackberry. HortScience, 39:316-320.
  • Mezzetti, B., Savini, G., Carnevali, F & Moti, D. (1997). Plant genotype and growth regulators interaction affecting in vitro morphogenesis of blackberry and raspberry. Biologia Plantarum, 39:139-150.
  • Muñoz-Concha, D., Quintero, J. & Ercişli, S. (2021). Media and hormones influence in micropropagation success of blackberry cv. ‘Chester’. Research Journal of Biotechnology. 16 (5):103-108.
  • Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum, 15:473-479.
  • Muratova, S. A. (2017). Biotekhnologicheskie aspekty razmnozheniya plodovyh i yagodnyh kul'tur. Sbornik nauchnyh trudov GNBS, 144, 2, 84–89 (in Russian).
  • McNicol, R. J. & Graham, J. (1990). In vitro regeneration of Rubus from leaf and stem segments. Plant cell tissue and organ culture, 21: 45–50.
  • Najaf-Abadi, A. J. & Hamidoghli, Y. (2009). Micropropagation of Thornless Trailing Blackberry (‘Rubus sp.’) by Axillary Bud Explants. Australian Journal of Crop Science, 3(4): 191-194.
  • De Oliveira, R. P. & Pacheco Nino, A. F. (2009). In vitro multiplication rate of raspberry cultivars. Rev. Bras. Frutic., Jaboticabal, 31, 1, 280-284 (in Italian).
  • Orlikowska, T. (1984). Micropropagation of Roodknop cv. black currant. Fruit Science Reports 11:15-17.
  • Petri, C. & Burgos, L (2005). Transformation of fruit trees: useful breeding tool or continued future prospect? Transgenic Research, 14:15-26.
  • Raeva-Bogoslovskaya, E N., Molkanova, O., Krakhmaleva, I. L. & Sobolev, E. V. (2021). Biotechnology methods to produce planting material of the genus Rubus L. IOP Conf. Series: Earth and Environmental Science, 941, 012027.
  • Shornikov, D. G., Bryuhina S. A., Muratova S. A., Yankovskaya M. B. & Papihin R. V. (2010). Optimizaciya uslovij kul'tivirovaniya invitro yagodnyh i dekorativnyh kul'tur. Vestnik TGU, t. 15, 2, 640–645 (in Russian).
  • Sigarroa-Rieche, A. & García-Delgado, C. (2011). Establecimiento y multiplicación in vitro de mora de castilla (Rubus glaucus Benth.) variedad sin espinas, mediante ápices meristemáticos. Acta Agron 60(4), 347-354.
  • Snedecor, G.W. & Cochran, W.G. (1967). Statistical Methods, ed. 6. Ames, Iowa, The Iowa State University Press.
  • Swartz, H.J., Bors, R., Mohamed, F. & Naes, S.K (1990). The effect of in vitropretreatments on subsequent shoot organogenesis from excised Rubusand Malus leaves. Plant Cell Tissue Organ Culture, 21:179- 184.
  • Tavartkiladze, O. K. & Vechernina, N. A. (2007). Razmnozhenie ezheviki v kul'ture in vitro. Biologicheskie nauki, 8, 28–30 (in Russian).
  • Turk, B.A., Swartz, H.J. & Zimmerman, R.H. (1994). Adventitious shoot regeneration from in vitro-cultured leaves of Rubusgenotypes. Plant Cell Tissue Organ Culture, 38:11-17.
  • Vaca, I. & Landázuri. y P. (2013). Evaluación de tres niveles de nitrógeno en medio de cultivo, en las fases de enraizamiento in vitro y adaptación a sustrato de Rubus glaucus (Benth). La Granja. Revista de Ciencias de la Vida, 18(2):48-54.
  • Villa, F., Pasqual, M., Asis, F.A., Las P. & Assis, G. A. (2007). In vitro blackberry growing: Effect of growth regulators and cultivar. Ciencia e Agrotecnologia 32:1754-1759.
  • Vujovic ́ , T., Ružic ́ , D., Cerovic ́ , R., Leposavic ́ , A., Karaklajic ́ -Stajic ́ , Z., Mitrovic ́ , O., Žurawicz, E. (2017). An assessment of the genetic integrity of micropropagated raspberry and blackberry plants. Sci. Hortic. 225, 454–461.
  • Wainwright, H. & Flegmann, A.W. (1986). Studies of the micropropagation of Ribes species. Acta Horticulturae, 183:315-322.
  • Wei, J., Gu Zhen, Y. & Zhi S. (1992). In vitro propagation of Rubus species. Scientia Horticulturae, 49, 3– 4, 335-340.
  • Wu, J.H., Miller, S.A., Hall, H.K, & Mooney, P.A. (2009). Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell Tissue Organ Culture 99:17- 25.
  • Zarei, A., Erfani-Moghadam, J. & Mozaffari. M. (2017). Phylogenetic analysis among some pome fruit trees of Rosaceae family using RAPD markers. Biotechnology & Biotechnological Equipment, 31(2):289-298.
  • Zawadzka, M. & Orlikowska, T. (2006). The influence of Fe EDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants. Plant Cell Tissue Organ Culture, 85:45-149.
APA TOPÇU H (2022). Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. , 205 - 217. 10.29329/ijiaar.2022.475.4
Chicago TOPÇU Hayat Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. (2022): 205 - 217. 10.29329/ijiaar.2022.475.4
MLA TOPÇU Hayat Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. , 2022, ss.205 - 217. 10.29329/ijiaar.2022.475.4
AMA TOPÇU H Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. . 2022; 205 - 217. 10.29329/ijiaar.2022.475.4
Vancouver TOPÇU H Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. . 2022; 205 - 217. 10.29329/ijiaar.2022.475.4
IEEE TOPÇU H "Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation." , ss.205 - 217, 2022. 10.29329/ijiaar.2022.475.4
ISNAD TOPÇU, Hayat. "Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation". (2022), 205-217. https://doi.org/10.29329/ijiaar.2022.475.4
APA TOPÇU H (2022). Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online), 6(3), 205 - 217. 10.29329/ijiaar.2022.475.4
Chicago TOPÇU Hayat Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online) 6, no.3 (2022): 205 - 217. 10.29329/ijiaar.2022.475.4
MLA TOPÇU Hayat Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online), vol.6, no.3, 2022, ss.205 - 217. 10.29329/ijiaar.2022.475.4
AMA TOPÇU H Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online). 2022; 6(3): 205 - 217. 10.29329/ijiaar.2022.475.4
Vancouver TOPÇU H Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online). 2022; 6(3): 205 - 217. 10.29329/ijiaar.2022.475.4
IEEE TOPÇU H "Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation." Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online), 6, ss.205 - 217, 2022. 10.29329/ijiaar.2022.475.4
ISNAD TOPÇU, Hayat. "Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation". Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online) 6/3 (2022), 205-217. https://doi.org/10.29329/ijiaar.2022.475.4
Sayfa Oluşturulma Tarihi
25-04-2024 5:23

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