Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury
Yıl: 2022 Cilt: 38 Sayı: 3 Sayfa Aralığı: 255 - 265 Metin Dili: İngilizce DOI: 10.47717/turkjsurg.2022.5620 İndeks Tarihi: 31-10-2022
Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury
Öz: Objective: Hepatic ischemia-reperfusion (IR) injury occurs in liver surgery, resection, and transplantation. Reactive oxygen species (ROS) produced following IR starts the cascade of cell damage, necrosis/apoptosis, and proinflammatory responses by activating intracellular signaling cascade to drive hepatocellular damage. Cerium oxide nanoparticles (CONPs) act as anti-inflammatory and antioxidant agents. Thus, we evaluated the protective effects of oral (o.g.) and intraperitoneal (i.p.) administration of CONPs on hepatic IR injury. Material and Methods: Mice were randomly divided into five groups: control, sham, IR protocol, CONP+IR (i.p.), and CONP+IR (o.g.). Mouse hepatic IR protocol was applied to the animals in the IR group. CONPs (300 μg/kg) were administered 24 hours before IR protocol. Blood and tissue samples were taken after the reperfusion period. Results: Hepatic IR injury markedly increased enzyme activities, tissue lipid peroxidation, myeloperoxidase (MPO), xanthine oxidase (XO), nitrite oxide (NO), and tissue nuclear factor kappa-B (NF-κB) p65 levels, plasma pro-inflammatory cytokines, chemokines, and adhesion molecules while decreasing antioxidant markers and caused pathological changes in hepatic tissue. The expression of tumor necrosis factor alpha (TNF-α), matrix metalloproteinase 2 (MMP-2), and 9 increased, and tissue inhibitor matrix metalloproteinase 1 (TIMP-1) expression decreased in the IR group. Pretreatment with CONPs o.g. and i.p. 24 hours before hepatic ischemia improved the biochemical parameters above and alleviated the histopathological findings. Conclusion: Results of the present study demonstrate a significant reduction in liver degeneration by administering CONPs via i.p. and o.g. route in an experimental liver IR model, suggesting that CONPs have the extensive potential to prevent hepatic IR injury.
Anahtar Kelime: Deneysel karaciğer iskemi-reperfüzyon hasarında cerium oksidin oral ve intraperitoneal uygulanmasının anti-enflamatuvar etkisi
Öz: Giriş ve Amaç: Hepatik iskemi-reperfüzyon (IR) hasarı karaciğer cerrahisi ve transplantasyonda meydana gelir. IR hücre hasarı kaskadını, nekroz/ apopitoz ve hepatosellüler hasarı yöneten intrasellüler sinyal kaskadını aktive olmasıyla oluşan proenflamatuvar cevaplar ile reaktif oksijen radikalleri (ROS) üretilir. Cerium oksit nanopartikülleri (CONPs) anti-enflamatuvar ve antioksidan ajan gibi davranmaktadır. Bu yüzden, CONPs’nin oral (o.g.) ve intraperitoneal (i.p.) uygulanmasının hepatic IR hasarındaki koruyucu etkisini değerlendirdik. Gereç ve Yöntem: Fareler rastgele kontrol, sham, IR protocol, CONP+ IR (i.p.) ve CONP (o.g.) olarak beş gruba ayrıldı. Fare hepatik IR protokolü IR grubundaki hayvanlara uygulandı. IR protokolünden 24 saat önce CONPs (300 μg/kg) uygulandı. Reperfüzyon periyodu sonrası kan ve doku örnekleri alındı. Bulgular: Hepatik IR hasarı, doku lipid peroksidasyonunu, miyeloperoksidaz (MPO), ksantin oksidaz (XO), nitrit oksit (NO) ve doku nükleer faktör kappa-B(NF-κB) p65 enzim aktivitelerinin seviyelerini belirgin şekilde arttırdı; plazma proenflamatuvar sitokinler, kemokinler ve adezyon molekülleri antioksidan belirteçleri azaltırken karaciğer dokusunda patolojik değişikliklere neden olmuştur. IR grubunda tümör nekroz faktör alfa (TNF-α), matriks metalloproteinaz 2 (MMP-2) ve 9 ekspresyonu artmış, doku inhibitörü matriks metalloproteinaz 1 (TIMP-1) ekspresyonu azalmıştır. Hepatik iskemiden 24 saat önce o.g. ve i.p. olarak uygulanan CONP’ler ile yukarıda belirtilen biyokimyasal parametreleri düzeldi ve histopatolojik özellikleri hafifletti. Sonuç: Bu çalışmanın sonuçları, deneysel bir karaciğer IR modelinde i.p. ve o.g. yoluyla CONP’lerin uygulanmasıyla karaciğer dejenerasyonunda önemli bir azalma olduğunu göstermektedir, bu da CONP’lerin hepatik IR hasarının önlenmesi için geniş bir potansiyele sahip olduğunu göstermektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Zengin A, Erikci A, TELLI G, GÜMÜSEL B, Kosemehmetoglu K, Ucar G, Algin M (2022). Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. , 255 - 265. 10.47717/turkjsurg.2022.5620 |
| Chicago | Zengin Akile,Erikci Acelya,TELLI GOKÇEN,GÜMÜSEL BÜLENT,Kosemehmetoglu Kemal,Ucar Gulberk,Algin Mustafa Cem Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. (2022): 255 - 265. 10.47717/turkjsurg.2022.5620 |
| MLA | Zengin Akile,Erikci Acelya,TELLI GOKÇEN,GÜMÜSEL BÜLENT,Kosemehmetoglu Kemal,Ucar Gulberk,Algin Mustafa Cem Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. , 2022, ss.255 - 265. 10.47717/turkjsurg.2022.5620 |
| AMA | Zengin A,Erikci A,TELLI G,GÜMÜSEL B,Kosemehmetoglu K,Ucar G,Algin M Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. . 2022; 255 - 265. 10.47717/turkjsurg.2022.5620 |
| Vancouver | Zengin A,Erikci A,TELLI G,GÜMÜSEL B,Kosemehmetoglu K,Ucar G,Algin M Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. . 2022; 255 - 265. 10.47717/turkjsurg.2022.5620 |
| IEEE | Zengin A,Erikci A,TELLI G,GÜMÜSEL B,Kosemehmetoglu K,Ucar G,Algin M "Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury." , ss.255 - 265, 2022. 10.47717/turkjsurg.2022.5620 |
| ISNAD | Zengin, Akile vd. "Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury". (2022), 255-265. https://doi.org/10.47717/turkjsurg.2022.5620 |
| APA | Zengin A, Erikci A, TELLI G, GÜMÜSEL B, Kosemehmetoglu K, Ucar G, Algin M (2022). Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. Turkish Journal of Surgery, 38(3), 255 - 265. 10.47717/turkjsurg.2022.5620 |
| Chicago | Zengin Akile,Erikci Acelya,TELLI GOKÇEN,GÜMÜSEL BÜLENT,Kosemehmetoglu Kemal,Ucar Gulberk,Algin Mustafa Cem Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. Turkish Journal of Surgery 38, no.3 (2022): 255 - 265. 10.47717/turkjsurg.2022.5620 |
| MLA | Zengin Akile,Erikci Acelya,TELLI GOKÇEN,GÜMÜSEL BÜLENT,Kosemehmetoglu Kemal,Ucar Gulberk,Algin Mustafa Cem Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. Turkish Journal of Surgery, vol.38, no.3, 2022, ss.255 - 265. 10.47717/turkjsurg.2022.5620 |
| AMA | Zengin A,Erikci A,TELLI G,GÜMÜSEL B,Kosemehmetoglu K,Ucar G,Algin M Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. Turkish Journal of Surgery. 2022; 38(3): 255 - 265. 10.47717/turkjsurg.2022.5620 |
| Vancouver | Zengin A,Erikci A,TELLI G,GÜMÜSEL B,Kosemehmetoglu K,Ucar G,Algin M Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury. Turkish Journal of Surgery. 2022; 38(3): 255 - 265. 10.47717/turkjsurg.2022.5620 |
| IEEE | Zengin A,Erikci A,TELLI G,GÜMÜSEL B,Kosemehmetoglu K,Ucar G,Algin M "Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury." Turkish Journal of Surgery, 38, ss.255 - 265, 2022. 10.47717/turkjsurg.2022.5620 |
| ISNAD | Zengin, Akile vd. "Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury". Turkish Journal of Surgery 38/3 (2022), 255-265. https://doi.org/10.47717/turkjsurg.2022.5620 |
