Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24

BELDÜZ KOLCU, Meral; Ay şal, Fulya; CANAKCI, Sabriye; BELDUZ, Ali Osman

doi:10.16984/saufenbilder.1074637

Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24

MERAL BELDÜZ KOLCU, (Avrasya Üniversitesi, Trabzon, Türkiye)

ALİ OSMAN BELDÜZ, (Karadeniz Teknik Üniversitesi, Trabzon, Türkiye)

Fulya AY ŞAL, (Karadeniz Teknik Üniversitesi, Trabzon, Türkiye)

Sabriye ÇANAKÇI (Karadeniz Teknik Üniversitesi, Trabzon, Türkiye)

Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi

5 1

Yıl: 2022 Cilt: 26 Sayı: 5 Sayfa Aralığı: 1956 - 1966 Metin Dili: İngilizce DOI: 10.16984/saufenbilder.1074637 İndeks Tarihi: 02-11-2022

Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24

Öz:

In this study, esterase of Aneurinibacillus sp. PDF24 strain, a thermophilic bacteria, was purified to homogenity (5.25 fold purification) by column chromotography, and characterized. The molecular weight of Aneurinibacillus sp. PDF24 esterase was determined about 40 kDa. The maximum activity of the purified esterase was analyzed at 55°C, pH 8.5. The esterase was found to be stable at 40ºC, 50ºC and 60ºC for 1 hour. Km and Vmax values for p-nitrophenyl butyrate were determined as 0.120 mM and 3164.8 U/mg, respectively. Considering Km values in the literature, Aneurinibacillus sp. PDF24 esterase was found to have a good Km value compared to other esterases. In the presence of 1 mM and 5 mM metal salts of Mg2+, Li+, Ca2+, K+, no significant change occured in enzyme activity. The activity of Aneurinibacillus sp PDF24 esterase was found to be stable also in the presence of ethanol, DMSO, EDTA, DTT and ß-mercaptoethanol. The data obtained suggest that the enzyme is a serine esterase, not a metalloprotein, and that disulfide bonds are not required to maintain enzyme conformation, and therefore, depending on its features, this esterase may be a suitable candidate for industrial applications.

Anahtar Kelime: Aneurinibacillus characterization esterase thermophilic

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BELDÜZ KOLCU M, BELDUZ A, Ay şal F, CANAKCI S (2022). Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. , 1956 - 1966. 10.16984/saufenbilder.1074637
Chicago	BELDÜZ KOLCU Meral,BELDUZ Ali Osman,Ay şal Fulya,CANAKCI Sabriye Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. (2022): 1956 - 1966. 10.16984/saufenbilder.1074637
MLA	BELDÜZ KOLCU Meral,BELDUZ Ali Osman,Ay şal Fulya,CANAKCI Sabriye Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. , 2022, ss.1956 - 1966. 10.16984/saufenbilder.1074637
AMA	BELDÜZ KOLCU M,BELDUZ A,Ay şal F,CANAKCI S Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. . 2022; 1956 - 1966. 10.16984/saufenbilder.1074637
Vancouver	BELDÜZ KOLCU M,BELDUZ A,Ay şal F,CANAKCI S Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. . 2022; 1956 - 1966. 10.16984/saufenbilder.1074637
IEEE	BELDÜZ KOLCU M,BELDUZ A,Ay şal F,CANAKCI S "Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24." , ss.1956 - 1966, 2022. 10.16984/saufenbilder.1074637
ISNAD	BELDÜZ KOLCU, Meral vd. "Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24". (2022), 1956-1966. https://doi.org/10.16984/saufenbilder.1074637

APA	BELDÜZ KOLCU M, BELDUZ A, Ay şal F, CANAKCI S (2022). Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 26(5), 1956 - 1966. 10.16984/saufenbilder.1074637
Chicago	BELDÜZ KOLCU Meral,BELDUZ Ali Osman,Ay şal Fulya,CANAKCI Sabriye Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 26, no.5 (2022): 1956 - 1966. 10.16984/saufenbilder.1074637
MLA	BELDÜZ KOLCU Meral,BELDUZ Ali Osman,Ay şal Fulya,CANAKCI Sabriye Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.26, no.5, 2022, ss.1956 - 1966. 10.16984/saufenbilder.1074637
AMA	BELDÜZ KOLCU M,BELDUZ A,Ay şal F,CANAKCI S Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2022; 26(5): 1956 - 1966. 10.16984/saufenbilder.1074637
Vancouver	BELDÜZ KOLCU M,BELDUZ A,Ay şal F,CANAKCI S Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2022; 26(5): 1956 - 1966. 10.16984/saufenbilder.1074637
IEEE	BELDÜZ KOLCU M,BELDUZ A,Ay şal F,CANAKCI S "Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24." Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 26, ss.1956 - 1966, 2022. 10.16984/saufenbilder.1074637
ISNAD	BELDÜZ KOLCU, Meral vd. "Characterization of a New Thermostable Carboxylesterase from Aneurinibacillus sp. PDF24". Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 26/5 (2022), 1956-1966. https://doi.org/10.16984/saufenbilder.1074637