TY  - JOUR
TI  - THE EFFECT OF HUMIC ACID ON BONE REGENERATION IN RATS
AB  - Objectives: The objective of our study was to investigate the effect of the local humic acid application in addition to autogenous bone grafting on the premature new bone amount. Materials and Methods: 12-week-old 24 Wistar rats were divided into 4 groups. Two parietal bone defects with 5-mm diameter were constituted in each animal. All defects in each group were treated with only autogenous bone graft (n=6), autogenous bone graft combined with 100 mg/kg (n=6) or 200 mg/kg humic acid (n=6). Defects in one group remained empty as control (n=6). The laboratory animals were sacrificed on the 28th day following the procedure. Morphological properties of all experimental defects were evaluated using micro-computed tomography (Micro- CT). Results: The highest value among trabecular thickness (Tb.Th) and the ratio of the bone volume to the tissue volume (BV/ TV) was encountered in the autogenous graft group applied with 100mg/kg humic acid. The highest value in the bone volume (BV) variance was detected in the group to which only the autogenous graft was applied. A statistically significant difference was found between the control group and the only autogenous graft-applied group and the autogenous graft group applied with local 100mg/kg humic acid upon comparing the groups in pairs for bone surface area (BS) variance. BS/BV value was found higher in the autogenous graft group applied with local 200mg/kg humic acid than the autogenous group applied with local 100mg/kg humic acid. Conclusions: Humic acid application in addition to autogenous bone grafting caused a decrease in the bone volume value. However, the positive effect of the humic acid application was observed in trabecular thickness and bone volume/tissue volume values, dosage increase negatively impacted the same. The dosage increase negatively affected the bone surface area value. No positive effect of the humic acid application to bone surface/bone volume value was observed. The statistical significance between the control group and the other groups is considered to be autogenous graft.
AU  - Şengül, Muhammet
DO  - 10.7126/cumudj.999234
PY  - 2021
JO  - Cumhuriyet Dental Journal 
VL  - 24
IS  - 4
SN  - 1302-5805
SP  - 361
EP  - 370
DB  - TRDizin
UR  - http://search/yayin/detay/1137310
ER  -