Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells

Tanrıverdi, Gamze; tanriverdi, bulent; Basaran, Serdar Hakan; Bayrak, Alkan; Avkan, Mustafa Cevdet

doi:10.14744/tjtes.2021.64569

Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells

Serdar Hakan BAŞARAN, (Ortopedi ve Travmatoloji Anabilim Dalı, İstanbul Bakırköy Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi, İstanbul-Türkiye)

Alkan Bayrak, (Ortopedi ve Travmatoloji Anabilim Dalı, İstanbul Bakırköy Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi, İstanbul-Türkiye)

Gamze Tanrıverdi, (Histoloji ve Embriyoloji Anabilim Dalı, İstanbul Üniversitesi-Cerrahpaşa, Cerrahpaşa Tıp Fakültesi, İstanbul-Türkiye)

Bülent TANRIVERDİ, (Ortopedi ve Travmatoloji Anabilim Dalı, İstanbul Bakırköy Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi, İstanbul-Türkiye)

Mustafa Cevdet Avkan (Ortopedi ve Travmatoloji Anabilim Dalı, İstanbul Bakırköy Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi, İstanbul-Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

0 0

Yıl: 2022 Cilt: 28 Sayı: 8 Sayfa Aralığı: 1066 - 1072 Metin Dili: İngilizce DOI: 10.14744/tjtes.2021.64569 İndeks Tarihi: 03-11-2022

Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells

Öz:

BACKGROUND: The autologous bone grafts still have been used as the gold standard to initiate and facilitate bone healing in cases with bone defects. Because of some disadvantages of autologous bone grafts, the new biocomposite grafts have been researched. The purpose of the present study was to investigate whether the bone marrow-derived mesenchymal stem cells (BM-MSCs) loaded into a biocomposite scaffold enhance bone regeneration. METHODS: In our study, a 10 mm osteoperiosteal segmental bone defect was created in the middle of the right and left ulnar bones of eight rabbits. The created defects were filled in the right ulnar bones of eight rabbits (Group I) with BM-MSCs loaded onto a bio- composite scaffold (Plexur PTM, Osteotech, Eatontown, NJ, USA) and in the other ulnar bones of the same rabbits (Group II) with only biocomposite graft. Radiographs of each forelimb were taken postoperatively at the end of the 6th week. Then, the rabbits were euthanized pharmacologically for histopathological evaluation. RESULTS: Were scored using a modified Lane and Sandhu scoring system. All defects healed in both groups. Radiological and histo- logical total scores were slightly better in Group I, but statistical tests did not reveal any significant differences between the two groups at the end of the 6th week radiologically and histologically (p>0.05). CONCLUSION: The results of our study demonstrated that in rabbit ulnar segmental bone defect model was obtained satisfactory regeneration with using biocomposite graft with or without BM-MSC

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Sen MK, Miclau T. Autologous iliac crest bone graft: Should it still be the gold standard for treating nonunions? Injury 2007;38:S75−80.
2. Younger EM, Chapman MW. Morbidity at bone graft donor sites. J Or- thop Trauma 1989;3:192−5.
3. Enneking WF, Eady JL, Burchardt H. Autogenous cortical bone grafts in the reconstruction of segmental skeletal defects. J Bone Joint Surg Am 1980;62:1039−58.
4. Ruhé PQ, Hedberg-Dirk EL, Padron NT, Spauwen PH, Jansen JA, Mikos AG. Porous poly (DL-lactic-co-glycolic acid)/calcium phos- phate cement composite for reconstruction of bone defects. Tissue Eng 2006;12:789−800.
5. Wang J, Yu X. Preparation, characterization and in vitro analysis of novel structured nanofibrous scaffolds for bone tissue engineering. Acta Bioma- ter 2010;6:3004−12.
6. Panetta NJ, Gupta DM, Quarto N, Longaker MT. Mesenchymal cells for skeletal tissue engineering. Panminerva Med 2009;51:25−41.
7. Waese EY, Kandel RA, Stanford WL. Application of stem cells in bone repair. Skeletal Radiol 2008;37:601−8.
8. Jones E, Yang X. Mesenchymal stem cells and bone regeneration: Current status. Injury 2011;42:562−8.
9. Laurencin CT, Khan Y, Kofron M, El-Amin S, Botchwey E, Yu X, et al. The ABJS Nicolas Andry award: Tissue engineering of bone and liga- ment: A 15-year perspective. Clin Orthop Relat Res 2006;447:221−36.
10. Bruder SP, Kurth AA, Shea M, Hayes WC, Jaiswal N, Kadiyala S. Bone regeneration by implantation of purified, culture-expanded human mes- enchymal stem cells. J Orthop Res 1998;16:155−62.
11. Kim H, Suh H, Jo SA, Kim HW, Lee JM, Kim EH et al. In vivo bone formation by human marrow stromal cells in biodegradable scaffolds that release dexamethasone and ascorbate-2-phosphate. Biochem Biophys Res Commun 2005;332:1053−60.
12. Hao W, Dong J, Jiang M, Wu J, Cui F, Zhou D. Enhanced bone formation in large segmental radial defects by combining adipose-derived stem cells expressing bone morphogenetic protein 2 with nHA/RHLC/PLA scaf- fold. Int Orthop 2010;34:1341−9.
13. Wan C, He Q, Li G. Allogenic peripheral blood derived mesenchymal stem cells (MSCs) enhance bone regeneration in rabbit ulna critical-sized bone defect model. J Orthop Res 2006;24:610−8.
14. Keskin DS, Tezcaner A, Korkusuz P, Korkusuz F, Hasirci V. Colla- gen-chondroitin sulfate-based PLLA-SAIB-coated rhBMP-2 delivery system for bone repair. Biomaterials 2005;26:4023−34.
15. Chen SH, Lei M, Xie XH, Zheng LZ, Yao D, Wang XL, et al. PLGA/ TCP composite scaffold incorporating bioactive phytomolecule icar- itin for enhancement of bone defect repair in rabbits. Acta Biomater 2013;9:6711−22.
16. Bostrom M, Lane JM, Tomin E, Browne M, Bernerian W, Turek T, et al. Use of bone morphogenetic protein-2 in the rabbit ulnar nonunion model. Clin Orthop Relat Res 1996;327:272−82.
17. Bolander ME, Glian G. The use of demineralize bone matrix in the repair of segmental defect. J Bone Joint Surg 1983;68A:1264–74.
18. Beloti MM, De Oliveira PT, De Carvalho PS, Rosa AL. Seeding osteo- blastic cells into a macroporous biodegradable CaP/PLGA scaffold by a centrifugal force. J Biomater Appl 2009;23:481−95.
19. Ngiam M, Liao S, Patil AJ, Cheng Z, Chan CK, Ramakrishna S. The fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nano- fibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering. Bone 2009;45:4−16.
20. Heiple KG, Golberg VM, Powell AE, Bos GD, Zika JM. Biology of can- cellous bone grafts. Orthop Clin North Am 1987;18:179−85.
21. Lane JM, Sandhu HS. Current approaches to experimental bone graft- ing. Orthop Clin North Am 1987;18:213−25.
22. Bigham AS, Dehghani SN, Shafiei Z, Nezhad ST. Xenogenic deminer- alized bone matrix and fresh autogenous cortical bone effects on experi- mental bone healing: Radiological, histopathological and biomechanical evaluation. J Orthop Traumatol 2008;9:73−80.
23. Goldstein SA, Patil PV, Moalli MR. Perspectives on tissue engineering of bone. Clin Orthop Relat Res 1999;367:S419−23.
24. Berkes MB, Little MT, Schottel PC, Pardee NC, Zuiderbaan A, Lazaro LE, et al. Outcomes of schatzker II tibial plateau fracture open reduc- tion internal fixation using structural bone allograft. J Orthop Trauma 2014;28:97−102.
25. Hersel U, Dahmen C, Kessler H. RGD modified polymers: Bio- materials for stimulated cell adhesion and beyond. Biomaterials 2003;24:4385−415.
26. Patterson TE, Kumagai K, Griffith L, Muschler GF. Cellular strategies for enhancement of fracture repair. J Bone Joint Surg Am 2008;90:111−9.
27. Janicki P, Schmidmaier G. What should be the characteristics of the ideal bone graft substitute? Combining scaffolds with growth factors and/or stem cells. Injury 2011;42:S77−81.
28. Akintoye SO, Lam T, Shi S, Brahim J, Collins MT, Robey PG. Skeletal site-specific characterization of orofacial and iliac crest human bone mar- row stromal cells in same individuals. Bone 2006;38:758−68.
29. Van Zant G, Liang Y. The role of stem cells in aging. Exp Hematol 2003;31:659−72.
30. Lucarelli E, Donati D, Cenacchi A, Fornasari PM. Bone reconstruction of large defects using bone marrow derived autologous stem cells. Transfus Apher Sci 2004;30:169−74.
31. Planka L, Gal P, Kecova H, Klima J, Hlucilova J, Filova E, et al. Allogeneic and autogenous transplantations of MSCs in treatment of the physeal bone bridge in rabbits. BMC Biotechnol 2008;12;8:70.
32. Giardino R, Aldini NN, Torricelli P, Fini M, Giavaresi G, Rocca M, et al. A resorbable biomaterial shaped as a tubular chamber and containing stem cells: A pilot study on artificial bone regeneration. Int J Artif Organs 2000;23:331−7.
33. Huibregtse BA, Johnstone B, Goldberg VM, Caplan AI. Effect of age and sampling site on the chondro-osteogenic potential of rabbit marrow-de- rived mesenchymal progenitor cells. J Orthop Res 2000;18:18−24.
34. Hernigou P, Poignard A, Beaujean F, Rouard H. Percutaneous autolo- gous bone-marrow grafting for nonunions. Influence of the number and concentration of progenitor cells. J Bone Joint Surg Am 2005;87:1430−7.
35. Bajada S, Harrison PE, Ashton BA, Cassar-Pullicino VN, Ashammakhi N, Richardson JB. Successful treatment of refractory tibial nonunion us- ing calcium sulphate and bone marrow stromal cell implantation. J Bone Joint Surg Br 2007;89:1382−6.
36. Marcacci M, Kon E, Moukhachev V, Lavroukov A, Kutepov S, Quarto R, et al. Successful treatment of refractory tibial nonunion using calci- um sulphate and bone marrow stromal cell implantation. Tissue Eng 2007;13:947−55.
37. Quarto R, Mastrogiacomo M, Cancedda, Kutepov SM, Mukhachev V, Lavroukov A, et al. Repair of large bone defects with the use of autolo- gous bone marrow stromal cells. N Engl J Med 2001;344:385−6.
38. Hao W, Pang L, Jiang M, Lv R, Xiong Z, Hu YY. Skeletal repair in rab- bits using a novel biomimetic composite based on adipose-derived stem cells encapsulated in collagen I gel with PLGA-beta-TCP scaffold. J Or- thop Res 2010;28:252−7.

APA	Basaran S, Bayrak A, Tanrıverdi G, tanriverdi b, Avkan M (2022). Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. , 1066 - 1072. 10.14744/tjtes.2021.64569
Chicago	Basaran Serdar Hakan,Bayrak Alkan,Tanrıverdi Gamze,tanriverdi bulent,Avkan Mustafa Cevdet Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. (2022): 1066 - 1072. 10.14744/tjtes.2021.64569
MLA	Basaran Serdar Hakan,Bayrak Alkan,Tanrıverdi Gamze,tanriverdi bulent,Avkan Mustafa Cevdet Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. , 2022, ss.1066 - 1072. 10.14744/tjtes.2021.64569
AMA	Basaran S,Bayrak A,Tanrıverdi G,tanriverdi b,Avkan M Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. . 2022; 1066 - 1072. 10.14744/tjtes.2021.64569
Vancouver	Basaran S,Bayrak A,Tanrıverdi G,tanriverdi b,Avkan M Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. . 2022; 1066 - 1072. 10.14744/tjtes.2021.64569
IEEE	Basaran S,Bayrak A,Tanrıverdi G,tanriverdi b,Avkan M "Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells." , ss.1066 - 1072, 2022. 10.14744/tjtes.2021.64569
ISNAD	Basaran, Serdar Hakan vd. "Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells". (2022), 1066-1072. https://doi.org/10.14744/tjtes.2021.64569

APA	Basaran S, Bayrak A, Tanrıverdi G, tanriverdi b, Avkan M (2022). Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. Ulusal Travma ve Acil Cerrahi Dergisi, 28(8), 1066 - 1072. 10.14744/tjtes.2021.64569
Chicago	Basaran Serdar Hakan,Bayrak Alkan,Tanrıverdi Gamze,tanriverdi bulent,Avkan Mustafa Cevdet Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. Ulusal Travma ve Acil Cerrahi Dergisi 28, no.8 (2022): 1066 - 1072. 10.14744/tjtes.2021.64569
MLA	Basaran Serdar Hakan,Bayrak Alkan,Tanrıverdi Gamze,tanriverdi bulent,Avkan Mustafa Cevdet Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. Ulusal Travma ve Acil Cerrahi Dergisi, vol.28, no.8, 2022, ss.1066 - 1072. 10.14744/tjtes.2021.64569
AMA	Basaran S,Bayrak A,Tanrıverdi G,tanriverdi b,Avkan M Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. Ulusal Travma ve Acil Cerrahi Dergisi. 2022; 28(8): 1066 - 1072. 10.14744/tjtes.2021.64569
Vancouver	Basaran S,Bayrak A,Tanrıverdi G,tanriverdi b,Avkan M Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells. Ulusal Travma ve Acil Cerrahi Dergisi. 2022; 28(8): 1066 - 1072. 10.14744/tjtes.2021.64569
IEEE	Basaran S,Bayrak A,Tanrıverdi G,tanriverdi b,Avkan M "Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells." Ulusal Travma ve Acil Cerrahi Dergisi, 28, ss.1066 - 1072, 2022. 10.14744/tjtes.2021.64569
ISNAD	Basaran, Serdar Hakan vd. "Partial load-bearing rabbit ulnar segmental defects are regenerated with biocompatible grafts with or without bone marrow-derived mesenchymal stem cells". Ulusal Travma ve Acil Cerrahi Dergisi 28/8 (2022), 1066-1072. https://doi.org/10.14744/tjtes.2021.64569