The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation

Mammadov, Renad; ELMA, BEKİR; Suleyman, Halis; Bilgin, Yasin; GÜNDOĞDU, BETÜL; ÇOBAN, T. Abdulkadir; Yurt, Şerif

doi:10.14744/tjtes.2021.97760

The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation

Bekir Elma, (Göğüs Cerrahisi Anabilim Dalı, Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi, Erzincan-Türkiye)

Renad Mammadov, (Farmakoloji Anabilim Dalı, Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi, Erzincan-Türkiye)

Halis Süleyman, (Farmakoloji Anabilim Dalı, Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi, Erzincan-Türkiye)

Betül Gündoğdu, (Atatürk Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı, Erzurum-Türkiye)

Şerif Yurt, (Mengücek Gazi Eğitim ve Araştırma Hastanesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Erzincan-Türkiye)

Yasin Bilgin, (Acil Servis, Mengücek Gazi Eğitim ve Araştırma Hastanesi, Erzincan-Türkiye)

Abdulkadir Çoban (Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi, Biyokimya Anabilim Dalı, Erzincan-Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

2 0

Yıl: 2022 Cilt: 28 Sayı: 8 Sayfa Aralığı: 1073 - 1081 Metin Dili: İngilizce DOI: 10.14744/tjtes.2021.97760 İndeks Tarihi: 03-11-2022

The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation

Öz:

BACKGROUND: Acute cardiac contusion induced by trauma is known with its high mortality and morbidity. The role of oxidative stress and inflammation in its pathophysiology has led to the investigation of antioxidant and anti-inflammatory substances in non-sur- gical treatment. In this study, the effects of rutin which has these two features on acute cardiac contusion were investigated. METHODS: Thirty male albino Wistar rats were divided into three equal groups as healthy (HG), contusion (CG), and rutin + con- tusion (rutin + CG). A heart contusion was created dropping 200 g weight from 1-m height onto anterior thorax of CG (n=10) and Rutin + CG (n=10) group animals by anesthetizing with intraperitoneal administration of 60 mg/kg ketamine and xylazine inhalation at appropriate intervals. Thirty minutes after contusion was applied, rutin at the dose of 50 mg/kg was administered orally to the stomach by gavage to the rutin + CG group animals. The rutin was used once a day for 2 days. Rats were killed at the end of 48 h. Heart tissues were removed and examined biochemically and histopathologically. Troponin I (TP I) and creatine kinase-MB (CK-MB) were measured in blood samples taken from the tail veins just before the rats were killed. RESULTS: TP I, CK-MB, malondialdehyde, total oxidant status, and nuclear factor-kappa B levels increased in the CG when compared to the HG, and Rutin application prevented this increase, total glutathione (eGSH) and total antioxidant status levels decreased, and rutin application prevented this decrease. Histopathological findings also supported these findings. CONCLUSION: Rutin had a protective effect on heart tissue.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Huis MA, Craft CA, Hood RE. Blunt cardiac trauma review. Cardiol Clin 2018;36:183 91.
2. Singh S, Heard M, Pester JM, Angus LD. Blunt cardiac injury. Treasure Island, FL: StatPearls Publishing; 2020.
3. Kamdar G, Santucci K, Emerson BL. Management of pediatric cardiac trauma in the ED. Clin Pediatr Emerg Med 2011;12:323−32.
4. Kuśmierczyk M, Drohomirecka A, Michałowska I, Michałek P, Ju- raszyński Z, Różański J. Delayed diagnosis of pericardial hematoma compressing the right ventricle after blunt chest trauma. J Card Surg 2013;28:701.
5. Wang J, Lu K, Liang F, Li X, Wang L, Yang C, et al. Decreased autophagy contributes to myocardial dysfunction in rats subjected to nonlethal me- chanical trauma. PLoS One 2013;8:e71400.
6. Tao L, Liu HR, Gao F, Qu Y, Christopher TA, Lopez BL, et al. Mechan- ical traumatic injury without circulatory shock causes cardiomyocyte apoptosis: Role of reactive nitrogen and reactive oxygen species. Am J Physiol Heart Circ Physiol 2005;288:H2811−8.
7. Kalbitz M, Amann EM, Bosch B, Palmer A, Schultze A, Pressmar J, et al. Experimental blunt chest trauma-induced myocardial inflamma- tion and alteration of gap-junction protein connexin 43. PLoS One 2017;12:e0187270.
8. Varela ML, Mogildea M, Moreno I, Lopes A. Acute inflammation and metabolism. Inflammation 2018;41:1115−27.
9. Ganeshpurkar A, Saluja AK. The pharmacological potential of rutin. Saudi Pharm J 2017;25:149−64.
10. Li M, Jiang Y, Jing W, Sun B, Miao C, Ren L. Quercetin provides greater cardioprotective effect than its glycoside derivative rutin on iso- proterenol-induced cardiac fibrosis in the rat. Can J Physiol Pharmacol 2013;91:951−9.
11. Guo R, Wei P, Liu W. Combined antioxidant effects of rutin and Vitamin C in Triton X-100 micelles. J Pharm Biomed Anal 2007;43:1580−6.
12. Lee W, Ku SK, Bae JS. Barrier protective effects of rutin in LPS-induced inflammation in vitro and in vivo. Food Chem Toxicol 2012;50:3048−55.
13. Chen WY, Huang YC, Yang ML, Lee CY, Chen CJ, Yeh CH, et al. Pro- tective effect of rutin on LPS-induced acute lung injury via down-regu- lation of MIP-2 expression and MMP-9 activation through inhibition of Akt phosphorylation. Int Immunopharmacol 2014;22:409−13.
14. Demiryilmaz I, Turan MI, Kisaoglu A, Gulapoglu M, Yilmaz I, Suley- man H. Protective effect of nimesulide against hepatic ischemia/reperfu- sion injury in rats: Effects on oxidant/antioxidants, DNA mutation and COX-1/COX-2 levels. Pharmacol Rep 2014;66:647−52.
15. Weber B, Lackner I, Haffner-Luntzer M, Palmer A, Pressmar J, Scharf- fetter-Kochanek K, et al. Modeling trauma in rats: Similarities to humans and potential pitfalls to consider. J Transl Med 2019;17:305.
16. Bradford MM. A rapid and sensitive method for the quantitation of mi- crogram quantities of protein utilizing the principle of protein-dye bind- ing. Anal Biochem 1976;72:248−54.
17. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351−8.
18. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonpro- tein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968;25:192−205.
19. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004;37:112−9.
20. Erel O. A new automated colorimetric method for measuring total oxi- dant status. Clin Biochem 2005;38:1103−11.
21. Leite L, Gonçalves L, Vieira DN. Cardiac injuries caused by trauma: Re- view and case reports. J Forensic Leg Med 2017;52:30−4.
22. Zhou T, Xiang D, Xie X. Effect of L-carnitine on heart function fol- lowing myocardial contusion in mongrel dogs. Chinese J Trauma 2015;31:1030−4.
23. Li S, Li X, Rozanski GJ. Regulation of glutathione in cardiac myocytes. J Mol Cell Cardiol 2003;35:1145−52.
24. Ceconi C, Bernocchi P, Boraso A, Cargnoni A, Pepi P, Curello S, et al. New insights on myocardial pyridine nucleotides and thiol redox state in ischemia and reperfusion damage. Cardiovasc Res 2000;47:586−94.
25. Watanabe K, Nagao M, Toh R, Irino Y, Shinohara M, Iino T, et al. Crit- ical role of glutamine metabolism in cardiomyocytes under oxidative stress. Biochem Biophys Res Commun 2021;534:687−93.
26. Aydin S, Kuloglu T, Aydin Y, Yalcin MH, Ugur K, Albayrak S, et al. Effects of iloprost and sildenafil treatment on elabela, apelin-13, nitric oxide, and total antioxidant and total oxidant status in experimental enzyme-positive acute coronary syndrome in rats. Biotech Histochem 2020;95:145−51.
27. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9−19.
28. Li X, Cao T, Ma S, Jing Z, Bi Y, Zhou J, et al. Curcumin ameliorates cardiac dysfunction induced by mechanical trauma. Eur J Pharmacol 2017;814:73−80.
29. Lin KH, Liu CL, Kuo WW, Paul CR, Chen WK, Wen SY, et al. Ear- ly fluid resuscitation by lactated Ringer’s solution alleviate the car- diac apoptosis in rats with trauma-hemorrhagic shock. PLoS One 2016;11:e0165406.
30. Jun L, Neng-Ping L, Yong-Feng G, Xin Y, Xiao-Bing L, Jian-Nong C, et al. Dynamic activity of NF-kB in multiple trauma patients and protective effects of ulinastain. Chin J Traumatol 2011;14:354−8.
31. Lawrence T. The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol 2009;1:a001651.
32. Oeckinghaus A, Ghosh S. The NF-κB family of transcription factors and its regulation. Cold Spring Harb Perspect Biol 2009;1:a000034.
33. Li H, Yang H, Wang D, Zhang L, Ma T. Peroxiredoxin2 (Prdx2) reduces oxidative stress and apoptosis of myocardial cells induced by acute myo- cardial infarction by inhibiting the TLR4/Nuclear factor kappa B (NF- κB) signaling pathway. Med Sci Monit 2020;26:e926281.
34. Joukar S, Najafipour H, Dabiri S, Sheibani M, Sharokhi N. Cardiopro- tective effect of mumie (shilajit) on experimentally induced myocardial injury. Cardiovasc Toxicol 2014;14:214−21.
35. York M, Scudamore C, Brady S, Chen C, Wilson S, Curtis M, et al. Char- acterization of troponin responses in isoproterenol-induced cardiac inju- ry in the Hanover Wistar rat. Toxicol Pathol 2007;35:606−17.
36. Dhivya V, Priya LB, Chirayil HT, Sathiskumar S, Huang CY, Padma VV. Piperine modulates isoproterenol induced myocardial ischemia through antioxidant and anti-dyslipidemic effect in male Wistar rats. Biomed Pharmacother 2017;87:705−13.
37. Bıçakçı N, Karaboğa İ, Dökmeci A, Güzel S, Erboğa ZF. Cardioprotec- tive effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in rats. Biotech Histochem 2019;94:442−8.
38. Demir F, Güzel A, Katı C, Karadeniz C, Akdemir U, Okuyucu A, et al. A combination of methylprednisolone and quercetin is effective for the treatment of cardiac contusion following blunt chest trauma in rats. Braz J Med Biol Res 2014;47:766−72.
39. Tousson E, Hafez E, Zaki S, Gad A. The cardioprotective effects of L-carnitine on rat cardiac injury, apoptosis, and oxidative stress caused by amethopterin. Environ Sci Pollut Res Int 2016;23:20600−8.
40. Guan DW, Zhang XG, Zhao R, Lu B, Han Y, Hou ZH, et al. Diverse morphological lesions and serious arrhythmias with hemodynamic in- sults occur in the early myocardial contusion due to blunt impact in dogs. Forensic Sci Int 2007;166:49−57.
41. Siti HN, Jalil J, Asmadi AY, Kamisah Y. Roles of rutin in cardiac remod- eling. J Funct Foods 2020;64:103606.
42. Xianchu L, Lan Z, Ming L, Yanzhi M. Protective effects of rutin on lipopolysaccharide-induced heart injury in mice. J Toxicol Sci 2018;43:329−37.
43. Umarani V, Muvvala S, Ramesh A, Lakshmi B, Sravanthi N. Rutin po- tentially attenuates fluoride-induced oxidative stress-mediated cardio- toxicity, blood toxicity and dyslipidemia in rats. Toxicol Mech Methods 2015;25:143−9.
44. Punithavathi V, Shanmugapriya K, Prince PS. Protective effects of rutin on mitochondrial damage in isoproterenol-induced cardiotoxic rats: An in vivo and in vitro study. Cardiovasc Toxicol 2010;10:181−9.
45. Annapurna A, Reddy CS, Akondi RB, Rao SR. Cardioprotective actions of two bioflavonoids, quercetin and rutin, in experimental myocardial in- farction in both normal and streptozotocin-induced Type I diabetic rats. J Pharm Pharmacol 2009;61:1365−74.
46. Bilgin AO, Mammadov R, Suleyman B, Unver E, Ozcicek F, Soyturk M, et al. Effect of rutin on cytarabine-associated pulmonary oedema and ox- idative stress in rats. An Acad Bras Cienc 2020;92:e20190261.
47. Imam F, Al-Harbi NO, Al-Harbia MM, Korashy HM, Ansari MA, Sayed-Ahmed MM, et al. Rutin attenuates carfilzomib-induced cardio- toxicity through inhibition of NF-κB, hypertrophic gene expression and oxidative stress. Cardiovasc Toxicol 2017;17:58−66.
48. Celik H, Kandemir FM, Caglayan C, Ozdemir S, Comaklı S, Kucukler S, et al. Neuroprotective effect of rutin against colistin-induced oxida- tive stress, inflammation and apoptosis in rat brain associated with the CREB/BDNF expressions. Mol Biol Rep 2020;47:2023−34.
49. Prince PS, Priya S. Preventive effects of rutin on lysosomal enzymes in isoproterenol induced cardio toxic rats: Biochemical, histological and in vitro evidences. Eur J Pharmacol 2010;649:229−35.
50. Topal I, Bilgin AO, Cimen FK, Kurt N, Suleyman Z, Bilgin Y, et al. The effect of rutin on cisplatin-induced oxidative cardiac damage in rats. Ana- tol J Cardiol 2018;20:136−42.
51. Mahmoud H, Ahmed O, Fahim H, Ahmed N, Ashour M. Effects of rutin and quercetin on doxorubicin-induced renocardiotoxicity in male Wistar rats. Adv Animal Vet Sci 2020;8:2020370−84.

APA	ELMA B, Mammadov R, Suleyman H, GÜNDOĞDU B, Yurt Ş, Bilgin Y, ÇOBAN T (2022). The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. , 1073 - 1081. 10.14744/tjtes.2021.97760
Chicago	ELMA BEKİR,Mammadov Renad,Suleyman Halis,GÜNDOĞDU BETÜL,Yurt Şerif,Bilgin Yasin,ÇOBAN T. Abdulkadir The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. (2022): 1073 - 1081. 10.14744/tjtes.2021.97760
MLA	ELMA BEKİR,Mammadov Renad,Suleyman Halis,GÜNDOĞDU BETÜL,Yurt Şerif,Bilgin Yasin,ÇOBAN T. Abdulkadir The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. , 2022, ss.1073 - 1081. 10.14744/tjtes.2021.97760
AMA	ELMA B,Mammadov R,Suleyman H,GÜNDOĞDU B,Yurt Ş,Bilgin Y,ÇOBAN T The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. . 2022; 1073 - 1081. 10.14744/tjtes.2021.97760
Vancouver	ELMA B,Mammadov R,Suleyman H,GÜNDOĞDU B,Yurt Ş,Bilgin Y,ÇOBAN T The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. . 2022; 1073 - 1081. 10.14744/tjtes.2021.97760
IEEE	ELMA B,Mammadov R,Suleyman H,GÜNDOĞDU B,Yurt Ş,Bilgin Y,ÇOBAN T "The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation." , ss.1073 - 1081, 2022. 10.14744/tjtes.2021.97760
ISNAD	ELMA, BEKİR vd. "The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation". (2022), 1073-1081. https://doi.org/10.14744/tjtes.2021.97760

APA	ELMA B, Mammadov R, Suleyman H, GÜNDOĞDU B, Yurt Ş, Bilgin Y, ÇOBAN T (2022). The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. Ulusal Travma ve Acil Cerrahi Dergisi, 28(8), 1073 - 1081. 10.14744/tjtes.2021.97760
Chicago	ELMA BEKİR,Mammadov Renad,Suleyman Halis,GÜNDOĞDU BETÜL,Yurt Şerif,Bilgin Yasin,ÇOBAN T. Abdulkadir The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. Ulusal Travma ve Acil Cerrahi Dergisi 28, no.8 (2022): 1073 - 1081. 10.14744/tjtes.2021.97760
MLA	ELMA BEKİR,Mammadov Renad,Suleyman Halis,GÜNDOĞDU BETÜL,Yurt Şerif,Bilgin Yasin,ÇOBAN T. Abdulkadir The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. Ulusal Travma ve Acil Cerrahi Dergisi, vol.28, no.8, 2022, ss.1073 - 1081. 10.14744/tjtes.2021.97760
AMA	ELMA B,Mammadov R,Suleyman H,GÜNDOĞDU B,Yurt Ş,Bilgin Y,ÇOBAN T The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. Ulusal Travma ve Acil Cerrahi Dergisi. 2022; 28(8): 1073 - 1081. 10.14744/tjtes.2021.97760
Vancouver	ELMA B,Mammadov R,Suleyman H,GÜNDOĞDU B,Yurt Ş,Bilgin Y,ÇOBAN T The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation. Ulusal Travma ve Acil Cerrahi Dergisi. 2022; 28(8): 1073 - 1081. 10.14744/tjtes.2021.97760
IEEE	ELMA B,Mammadov R,Suleyman H,GÜNDOĞDU B,Yurt Ş,Bilgin Y,ÇOBAN T "The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation." Ulusal Travma ve Acil Cerrahi Dergisi, 28, ss.1073 - 1081, 2022. 10.14744/tjtes.2021.97760
ISNAD	ELMA, BEKİR vd. "The effect of rutin on experimentally induced acute heart contusion in rats: Biochemical and histopathological evaluation". Ulusal Travma ve Acil Cerrahi Dergisi 28/8 (2022), 1073-1081. https://doi.org/10.14744/tjtes.2021.97760