Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir

Merve ATALAY, (Gıda Mühendisliği Bölümü, Mühendislik Fakültesi, İnönü Üniversitesi, 44280 Malatya, TÜRKİYE)
Didem Sahingil (Gıda Mühendisliği Bölümü, Mühendislik Fakültesi, İnönü Üniversitesi, 44280 Malatya, TÜRKİYE)
Tarım Bilimleri Dergisi
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Yıl: 2022 Cilt: 28 Sayı: 3 Sayfa Aralığı: 370 - 384 Metin Dili: İngilizce DOI: 10.15832/ankutbd.880744 İndeks Tarihi: 07-11-2022

Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir

Öz:
In the present study, bioactive properties such as ACE-I activity and antimicrobial activity of kefir using different Lactobacillus (Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842, Lactobacillus helveticus ATCC 15009 and Lactobacillus plantarum ATCC 14917) on some pathogen and Gram-positive bacteria during 28 day-storage periods was investigated and proteolysis (RP-HPLC peptide profiles, RP-HPLC amino acid profiles) were studied. The antimicrobial activity was investigated in kefir extract and separated peptide fractions (<3 kDa, named F2) which were characterized by LC-MS/MS and precursor and product ions were determined. Antimicrobial activity has been observed against pathogenic bacteria such as Escherichia coli (E. coli) in all samples. But the results revealed that the F2 fraction separated from kefir manufactured using Lactobacillus had a stronger antibacterial effect than control samples. It was determined that the F2 fraction has antimicrobial activity against S. aureus, S. warneri 95052 and S. hominis. The ACE-I activity of samples A, B, C and K were 76.47%, 84.95%, 87.33% and 85.57%, respectively. In the kefir using Lactobacillus has increase of ACE-I activity and was significant (P<0.01). In this study, it was concluded that the using of adjunct Lactobacillus contributed to the functional value of kefir.
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APA atalay m, Sahingil D (2022). Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. , 370 - 384. 10.15832/ankutbd.880744
Chicago atalay merve,Sahingil Didem Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. (2022): 370 - 384. 10.15832/ankutbd.880744
MLA atalay merve,Sahingil Didem Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. , 2022, ss.370 - 384. 10.15832/ankutbd.880744
AMA atalay m,Sahingil D Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. . 2022; 370 - 384. 10.15832/ankutbd.880744
Vancouver atalay m,Sahingil D Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. . 2022; 370 - 384. 10.15832/ankutbd.880744
IEEE atalay m,Sahingil D "Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir." , ss.370 - 384, 2022. 10.15832/ankutbd.880744
ISNAD atalay, merve - Sahingil, Didem. "Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir". (2022), 370-384. https://doi.org/10.15832/ankutbd.880744
APA atalay m, Sahingil D (2022). Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. Tarım Bilimleri Dergisi, 28(3), 370 - 384. 10.15832/ankutbd.880744
Chicago atalay merve,Sahingil Didem Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. Tarım Bilimleri Dergisi 28, no.3 (2022): 370 - 384. 10.15832/ankutbd.880744
MLA atalay merve,Sahingil Didem Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. Tarım Bilimleri Dergisi, vol.28, no.3, 2022, ss.370 - 384. 10.15832/ankutbd.880744
AMA atalay m,Sahingil D Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. Tarım Bilimleri Dergisi. 2022; 28(3): 370 - 384. 10.15832/ankutbd.880744
Vancouver atalay m,Sahingil D Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir. Tarım Bilimleri Dergisi. 2022; 28(3): 370 - 384. 10.15832/ankutbd.880744
IEEE atalay m,Sahingil D "Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir." Tarım Bilimleri Dergisi, 28, ss.370 - 384, 2022. 10.15832/ankutbd.880744
ISNAD atalay, merve - Sahingil, Didem. "Characterization of Antimicrobial Peptide Fraction Producing Lactobacillus spp. Based on LC/MS-MS and Determination of ACE-inhibitory Activity in Kefir". Tarım Bilimleri Dergisi 28/3 (2022), 370-384. https://doi.org/10.15832/ankutbd.880744
Sayfa Oluşturulma Tarihi
26-04-2024 11:41

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