An investigation of the effect of acrylamide on fracture healing in rats

Gumus, Erkan; Bulut, Okay; kılınç, seyran; Keklikcioğlu Çakmak, Neşe; TAŞ, Ayca; PAZARCI, Özhan; Yıldız, Şeyma Nur

doi:10.14744/tjtes.2021.94848

An investigation of the effect of acrylamide on fracture healing in rats

Özhan PAZARCI, (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Sivas, Türkiye)

Erkan GÜMÜŞ, (Aydın Adnan Menderes Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı, Aydın, Türkiye)

AYÇA TAŞ, (Sivas Cumhuriyet Üniversitesi Sağlık Bilimleri Fakültesi, Beslenme ve Diyet Bölümü, Sivas, Türkiye)

Neşe KEKLİKCİOĞLU ÇAKMAK, (Sivas Cumhuriyet Üniversitesi Mühendislik Fakültesi, Kimya Mühendisliği Bölümü, Sivas, Türkiye)

Seyran KILINÇ, (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Sivas, Türkiye)

Şeyma Nur YILDIZ, (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Biyokimya Anabilim Dalı, Sivas, Türkiye)

Okay BULUT (Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Sivas, Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

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Yıl: 2022 Cilt: 28 Sayı: 5 Sayfa Aralığı: 570 - 578 Metin Dili: İngilizce DOI: 10.14744/tjtes.2021.94848 İndeks Tarihi: 07-11-2022

An investigation of the effect of acrylamide on fracture healing in rats

Öz:

BACKGROUND: The aim of this study was to investigate the effects of acrylamide (AA) on fracture healing histologically, biochemically, and radiologically in a rat femur fracture model. METHODS: Scanning electron microscopy imaging, Fourier transform infrared spectroscopy, and ultraviolet-visible spectrophotometer examination were performed for AA characterization. In this study, after the femur fracture model was created, the groups were formed to include eight rats in each group (G) as follows: G1: 15th-day control, G2: 15th-day AA, G3: 30th-day control, and G4: 30th-day AA. In G2 and G4, 5 mg/kg AA was administered 3 times a week by gastric gavage. The fracture was evaluated radiologically according to Lane-Sandhu scoring and histologically according to Huo scoring. The weight changes of the rats were recorded. Albumin, total protein, cholesterol, high-density lipoprotein, low-density lipoprotein, triglyceride, alkaline phosphatase, lactate dehydrogenase, vit. D, parathormone, calcium, phosphorus (P), white blood cell (WBC), hemoglobin (Hb), and Platelet values were examined in the blood samples. RESULTS: The characterization properties of AA were confirmed. No significant weight change was observed in the rats during the study. When blood values were compared, a statistically significant difference was determined between albumin, total protein, P, WBC, and Hb groups (p=0.41, p=0.00, p=0.003, p=0.019, and p=0.017, respectively). According to the histological score comparisons, G3 was significantly different from G1, G2, and G4 (p<0.05), and G4 was significantly different from G1 and G2 (p<0.05). According to Lane-Sandhu scoring, there was a significant difference between G2 and G3 and G4 (p=0.0, p=0.034), G1 and G3 (p=0.001), respectively. CONCLUSION: AA adversely affects fracture healing even at low doses, as in the present study. According to the results of this study, the authors recommend a diet poor in AA during fracture treatment. Therefore, further human studies are required to find out the complex effect of AA on bone healing and the body.

Anahtar Kelime:

Akrilamidin sıçanlarda kırık iyileşmesi üzerindeki etkisinin araştırılması

Öz:

AMAÇ: Bu çalışmanın amacı, bir sıçan femur kırığı modelinde akrilamidin (AA) histolojik, biyokimyasal ve radyolojik olarak kırık iyileşmesi üzerindeki etkilerini araştırmaktır. GEREÇ VE YÖNTEM: Akrilamid karakterizasyonu için taramalı elektron mikroskobu (SEM) görüntüleme ve Fourier dönüşüm kızılötesi spektroskopi (FTIR) ve UV (ultraviyole) -Vis (görünür) spektrofotometre incelemesi yapıldı. Bu çalışmada, femur kırığı modeli oluşturulduktan sonra gruplar, her grupta (G) sekiz sıçan olacak şekilde oluşturuldu; G1: 15. gün kontrol, G2: 15. gün AA, G3: 30. gün kontrol, G4: 30. gün AA. G2 ve G4’te 5 mg/ kg akrilamid, mide gavajı ile haftada üç kez uygulandı. Kırık, Lane-Sandhu skorlamasına göre radyolojik olarak ve Huo skorlamasına göre histolojik olarak değerlendirildi. Sıçanların ağırlık değişiklikleri kaydedildi. Kan örneklerinde; albümin, total protein, kolesterol, HDL, LDL, trigliserit, ALP, LDH, vitamin D, PTH, Ca, P, WBC, Hb, Plt değerleri incelendi. BULGULAR: Akrilamidin karakterizasyon özellikleri doğrulandı. Çalışma sırasında sıçanlarda önemli bir ağırlık değişikliği gözlenmedi. Kan değerleri karşılaştırıldığında albümin, total protein, fosfor, WBC ve hemoglobin grupları arasında istatistiksel olarak anlamlı bir fark tespit edildi (sırasıyla, p=0.41, p=0.00, p= 0.003, p=0.019 ve p=0.017). Histolojik skor karşılaştırmalarına göre G3, G1, G2 ve G4’ten anlamlı olarak farklıydı (p<0.05) ve G4, G1 ve G2’den anlamlı olarak farklıydı (p<0.05). Lane-Sandhu skorlamasına göre sırasıyla G2 ile G3 ve G4 (p=0.0, p=0.034), G1 ve G3 (p=0.001) arasında anlamlı fark vardı. TARTIŞMA: Akrilamidin çalışmamızda olduğu gibi düşük dozlarda bile kırık iyileşmesini olumsuz etkilemektedir. Bu çalışmanın sonuçlarına göre, yazarlar, kırık tedavisi sırasında AA bakımından fakir bir diyet önermektedir. AA’nın kemik iyileşmesi ve vücut üzerindeki karmaşık etkisini bulmak için daha fazla insan çalışmasına ihtiyaç vardır

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	PAZARCI Ö, Gumus E, TAŞ A, Keklikcioğlu Çakmak N, kılınç s, Yıldız Ş, Bulut O (2022). An investigation of the effect of acrylamide on fracture healing in rats. , 570 - 578. 10.14744/tjtes.2021.94848
Chicago	PAZARCI Özhan,Gumus Erkan,TAŞ Ayca,Keklikcioğlu Çakmak Neşe,kılınç seyran,Yıldız Şeyma Nur,Bulut Okay An investigation of the effect of acrylamide on fracture healing in rats. (2022): 570 - 578. 10.14744/tjtes.2021.94848
MLA	PAZARCI Özhan,Gumus Erkan,TAŞ Ayca,Keklikcioğlu Çakmak Neşe,kılınç seyran,Yıldız Şeyma Nur,Bulut Okay An investigation of the effect of acrylamide on fracture healing in rats. , 2022, ss.570 - 578. 10.14744/tjtes.2021.94848
AMA	PAZARCI Ö,Gumus E,TAŞ A,Keklikcioğlu Çakmak N,kılınç s,Yıldız Ş,Bulut O An investigation of the effect of acrylamide on fracture healing in rats. . 2022; 570 - 578. 10.14744/tjtes.2021.94848
Vancouver	PAZARCI Ö,Gumus E,TAŞ A,Keklikcioğlu Çakmak N,kılınç s,Yıldız Ş,Bulut O An investigation of the effect of acrylamide on fracture healing in rats. . 2022; 570 - 578. 10.14744/tjtes.2021.94848
IEEE	PAZARCI Ö,Gumus E,TAŞ A,Keklikcioğlu Çakmak N,kılınç s,Yıldız Ş,Bulut O "An investigation of the effect of acrylamide on fracture healing in rats." , ss.570 - 578, 2022. 10.14744/tjtes.2021.94848
ISNAD	PAZARCI, Özhan vd. "An investigation of the effect of acrylamide on fracture healing in rats". (2022), 570-578. https://doi.org/10.14744/tjtes.2021.94848

APA	PAZARCI Ö, Gumus E, TAŞ A, Keklikcioğlu Çakmak N, kılınç s, Yıldız Ş, Bulut O (2022). An investigation of the effect of acrylamide on fracture healing in rats. Ulusal Travma ve Acil Cerrahi Dergisi, 28(5), 570 - 578. 10.14744/tjtes.2021.94848
Chicago	PAZARCI Özhan,Gumus Erkan,TAŞ Ayca,Keklikcioğlu Çakmak Neşe,kılınç seyran,Yıldız Şeyma Nur,Bulut Okay An investigation of the effect of acrylamide on fracture healing in rats. Ulusal Travma ve Acil Cerrahi Dergisi 28, no.5 (2022): 570 - 578. 10.14744/tjtes.2021.94848
MLA	PAZARCI Özhan,Gumus Erkan,TAŞ Ayca,Keklikcioğlu Çakmak Neşe,kılınç seyran,Yıldız Şeyma Nur,Bulut Okay An investigation of the effect of acrylamide on fracture healing in rats. Ulusal Travma ve Acil Cerrahi Dergisi, vol.28, no.5, 2022, ss.570 - 578. 10.14744/tjtes.2021.94848
AMA	PAZARCI Ö,Gumus E,TAŞ A,Keklikcioğlu Çakmak N,kılınç s,Yıldız Ş,Bulut O An investigation of the effect of acrylamide on fracture healing in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2022; 28(5): 570 - 578. 10.14744/tjtes.2021.94848
Vancouver	PAZARCI Ö,Gumus E,TAŞ A,Keklikcioğlu Çakmak N,kılınç s,Yıldız Ş,Bulut O An investigation of the effect of acrylamide on fracture healing in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2022; 28(5): 570 - 578. 10.14744/tjtes.2021.94848
IEEE	PAZARCI Ö,Gumus E,TAŞ A,Keklikcioğlu Çakmak N,kılınç s,Yıldız Ş,Bulut O "An investigation of the effect of acrylamide on fracture healing in rats." Ulusal Travma ve Acil Cerrahi Dergisi, 28, ss.570 - 578, 2022. 10.14744/tjtes.2021.94848
ISNAD	PAZARCI, Özhan vd. "An investigation of the effect of acrylamide on fracture healing in rats". Ulusal Travma ve Acil Cerrahi Dergisi 28/5 (2022), 570-578. https://doi.org/10.14744/tjtes.2021.94848