Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer

Ebru DEVECİ, (Konya Teknik Üniversitesi, Teknik Bilimler Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri Bölümü, Konya, Türkiye)
Gülsen TEL ÇAYAN, (Muğla Sıtkı Koçman Üniversitesi, Muğla Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri Bölümü, Muğla, Türkiye)
Mehmet Emin DURU (Muğla Sıtkı Koçman Üniversitesi, Kimya Bölümü, Fen Fakültesi, Muğla, Türkiye)
MANTAR DERGİSİ
8 2
Yıl: 2022 Cilt: 13 Sayı: 2 Sayfa Aralığı: 112 - 119 Metin Dili: İngilizce İndeks Tarihi: 16-11-2022

Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer

Öz:
Phellinus species are in the classification of valuable mushrooms among medicinal mushrooms and have been the source of traditional medicine for centuries. In this study, the hexane (PPH), methanol (PPM) and water (PPW) extracts, fractions and isolated compounds from Porodaedalea pini (Çamkavı mantarı) were screened for their cytotoxic effects on PC3 (prostate cancer) and 3T3 (murine fibroblast) cell lines by MTT assay. PPH was found to have moderate cytotoxicity on PC3 cell with the IC50 values of 33.84±0.01 µg/mL. The fatty acid profile of PPH was identified by GC-FID (gas chromatography-flame ionization dedector) and GC-MS (gas chromatography-mass spectrometry) and the main fatty acids were recorded as palmitic (41.40±0.03%), linoleic (21.53±0.01%), oleic (12.06±0.01%), and stearic (8.20±0.01%) acids. PPM and PPW were fractioned by using liquid-liquid extractions. Among all fractions, the 2-butanol fraction of the methanol extract (PPM-B) indicated the best cytotoxicity on PC3 and 3T3 cell lines. Also, among the isolated compounds (dioctyl phthalate (1), ergosterol peroxide (2) and pinoresinol (3)) from PPM, ergosterol peroxide (2) was found as moderate cytotoxic on PC3 with the IC50 value of 95.47±1.01 µg/mL. This study, in which the effect of P. pini on PC3 cell was examined for the first time, proved its medicinal importance by revealing the cytotoxic properties of the species.
Anahtar Kelime: Porodaedalea pini Prostate cancer Extract Fatty acid Isolation

Porodaedalea pini Mantarının Prostat Kanseri Üzerindeki Sitotoksik Etkisinin Değerlendirilmesi

Öz:
Phellinus türleri şifalı mantarlar arasındaki değerli mantarlar sınıfında yer almaktadır ve yüzyıllardır geleneksel tıbbın kaynağı olmuştur. Bu çalışmada, Porodaedalea pini (Çamkavı mantarı)'den elde edilen hekzan (PPH), metanol (PPM) ve su (PPW) ekstrelerinin, fraksiyonlarının ve izole edilmiş bileşiklerin PC3 (prostat kanseri) ve 3T3 (fare fibroblast) hücre hatları üzerindeki sitotoksik etkileri MTT testi kullanılarak taranmıştır. PPH’ın 33.84±0.01 µg/mL olan IC50 değeri ile PC3 üzerinde orta derecede sitotoksisiteye sahip olduğu bulunmuştur. PPH’ın yağ asidi profili GC-FID (gaz kromatografisi-alev iyonizasyon dedektörü) ve GC-MS (gaz kromatografisi-kütle spektrometresi) ile belirlenmiş ve majör yağ asitleri palmitik (%41.40±0.03), linoleik (%21.53±0.01), oleik (%12.06±0.01) ve stearik (%8.20±0.01) asit olarak kaydedilmiştir. PPM ve PPW ekstreleri, sıvı-sıvı ekstraksiyon tekniği kullanılarak fraksiyonlandırılmıştır. Tüm fraksiyonlar arasında metanol ekstresinden elde edilen 2-bütanol fraksiyonu (PPM-B) PC3 ve 3T3 hücre hatlarında en iyi sitotoksisiteyi göstermiştir. Ayrıca, PPM’den izole edilen bileşiklerden (dioktil ftalat (1), ergosterol peroksit (2) ve pinoresinol (3)), ergosterol peroksit (2) 95.47±1.01 µg/mL olan IC50 değeri ile PC3 hücre hattında orta derecede sitotoksik olarak bulunmuştur. P. pini'nin prostat kanseri üzerindeki etkisinin ilk kez incelendiği bu çalışmada, türün sitotoksik özellikleri ortaya çıkartılarak tıbbi önemi kanıtlanmıştır.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Deveci E, Tel-Çayan G, DURU M (2022). Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. , 112 - 119.
Chicago Deveci Ebru,Tel-Çayan Gulsen,DURU Mehmet Emin Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. (2022): 112 - 119.
MLA Deveci Ebru,Tel-Çayan Gulsen,DURU Mehmet Emin Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. , 2022, ss.112 - 119.
AMA Deveci E,Tel-Çayan G,DURU M Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. . 2022; 112 - 119.
Vancouver Deveci E,Tel-Çayan G,DURU M Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. . 2022; 112 - 119.
IEEE Deveci E,Tel-Çayan G,DURU M "Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer." , ss.112 - 119, 2022.
ISNAD Deveci, Ebru vd. "Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer". (2022), 112-119.
APA Deveci E, Tel-Çayan G, DURU M (2022). Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. MANTAR DERGİSİ, 13(2), 112 - 119.
Chicago Deveci Ebru,Tel-Çayan Gulsen,DURU Mehmet Emin Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. MANTAR DERGİSİ 13, no.2 (2022): 112 - 119.
MLA Deveci Ebru,Tel-Çayan Gulsen,DURU Mehmet Emin Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. MANTAR DERGİSİ, vol.13, no.2, 2022, ss.112 - 119.
AMA Deveci E,Tel-Çayan G,DURU M Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. MANTAR DERGİSİ. 2022; 13(2): 112 - 119.
Vancouver Deveci E,Tel-Çayan G,DURU M Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer. MANTAR DERGİSİ. 2022; 13(2): 112 - 119.
IEEE Deveci E,Tel-Çayan G,DURU M "Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer." MANTAR DERGİSİ, 13, ss.112 - 119, 2022.
ISNAD Deveci, Ebru vd. "Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer". MANTAR DERGİSİ 13/2 (2022), 112-119.
Sayfa Oluşturulma Tarihi
18-04-2024 5:43

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