TY  - JOUR
TI  - Phytochemical Analysis and Screening of Acetylcholinesterase and Carbonic Anhydrase I and II Isoenzymes Inhibitory Effect of Heptaptera triquetra (Vent.) Tutin Root
AB  - Alzheimer’s disease (AD) is characterized by progressive memory loss, deterioration of other cognitive functions, and inability to perform activities of daily living. Inhibiting the acetylcholinesterase (AChE) enzyme causes Ach accumulation in cholinergic synapses. This situation is expected to increase cognitive functions. Carbonic anhydrase enzymes (CAs) are ubiquitous in all living organisms. They have crucial physiological and pathological roles. CA inhibitors (CAIs) bind to catalytic zinc ions in the active site of CA isoenzymes and block their activity. The clinical use of CAIs had been established as antiglaucoma, anticonvulsant agents, diuretics, and anti-obesity drugs, in managing mountain sickness, gastric and duodenal ulcers, neurological disorders, osteoporosis, and tumors. To evaluate the bioactive profile of Heptaptera triquetra root, isolation studies, AChE, and human carbonic anhydrase (hCA) I and II inhibitory activities were performed. According to isolation studies, one fatty acid, coniferyl palmitate (1); four sesquiterpene coumarins, umbelliprenin (2), badrakemin acetate (4), colladonin (5), karatavicinol (6); and two sterols, stigmasterol (3a), β-sitosterol (3b) were isolated. All isolated compounds showed high potency against all enzymes (except badrakemin acetate for AChE) compared to standards. Umbelliprenin (2) with an IC50 value of 31.500 nM against hCA I, colladonin (5) with an IC50 value of 36.473 nM against hCA II and stigmasterol (3a), and β-sitosterol (3b) mixture with an IC50 value 9.000 nM against AChE demonstrated the best activity
AU  - ÇİÇEK KAYA, Ayşe
AU  - YUCA, Hafize
AU  - Güvenalp, Zühal
AU  - Yılmaz, Gülderen
AU  - bingöl, zeynebe
AU  - Özbek, Hilal
AU  - Gulcin, ilhami
AU  - Kazaz, Cavit
DO  - 10.55262/fabadeczacilik.1147174
PY  - 2022
JO  - FABAD Journal of Pharmaceutical Sciences
VL  - 47
IS  - 3
SN  - 1300-4182
SP  - 381
EP  - 392
DB  - TRDizin
UR  - http://search/yayin/detay/1140438
ER  -