THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS
Yıl: 2022 Cilt: 5 Sayı: 3 Sayfa Aralığı: 142 - 147 Metin Dili: İngilizce DOI: 10.53446/actamednicomedia.1166837 İndeks Tarihi: 06-12-2022
THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS
Öz: Objective: The treatment of prostate cancer has not significantly advanced despite several research to enhance early diagnosis and the introduction of innovative therapy agents. Prostate cancer metastasis is also a significant obstacle to treatment approaches. Therefore, the aim of the current investigation was to examine how the non-steroidal anti-inflammatory medicine meclofenamic acid (MA) affected the invasion and migration of LNCaP prostate cancer cells. Methods: Firstly, the non-toxic concentrations of MA on LNCaP cells were determined by trypan blue exclusion assay. After that, the effect of MA on migration and invasion was evaluated by wound healing assay and matrigel invasion assay, respectively. Finally, western blotting was used to determine the vimentin expression level, a marker for the transition of epithelial to mesenchymal cells. Results: The results of trypan blue exclusion assay showed that 60 and 80 µM concentrations of MA were non-toxic to the cells. The migration rate of non-treated control cells was 44.4%, while it was significantly reduced to 12.9% and 2.9% in 60 and 80 µM MAtreated groups, respectively. When the cells were treated with 80 µM MA, it significantly reduced the invasion of LNCaP cells from 52% to 30.3%. Vimentin expression was considerably decreased in MA-treated cells, according to the results of a western blot. Conclusion: This study demonstrates for the first time that MA inhibits the migration and invasion of prostate cancer LNCaP cells by decreasing the expression level of vimentin.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Rawla P. Epidemiology of Prostate Cancer. World J Oncol. Apr 2019;10(2):63-89. doi:10.14740/wjon1191
- 2. Xu S, Zhang Z, Ogawa O, et al. An EP4 antagonist ONO-AE3- 208 suppresses cell invasion, migration, and metastasis of prostate cancer. Cell Biochem Biophys. Sep 2014;70(1):521-7. doi:10.1007/s12013-014-9951-2
- 3. De U, Kundu S, Patra N, et al. A New Histone Deacetylase Inhibitor, MHY219, Inhibits the Migration of Human Prostate Cancer Cells via HDAC1. Biomol Ther (Seoul). Sep 2015;23(5):434-41. doi:10.4062/biomolther.2015.026
- 4. Jeong JW, Jin CY, Park C, et al. Inhibition of migration and invasion of LNCaP human prostate carcinoma cells by cordycepin through inactivation of Akt. Int J Oncol. May 2012;40(5):1697-704. doi:10.3892/ijo.2012.1332
- 5. Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646-74. doi:10.1016/j.cell. 2011.02.013
- 6. Chattopadhyay I, Ambati R, Gundamaraju R. Exploring the Crosstalk between Inflammation and EpithelialMesenchymal Transition in Cancer. Mediators Inflamm. 2021;2021:9918379. doi:10.1155/2021/9918379
- 7. Markopoulos GS, Roupakia E, Marcu KB, Kolettas E. Epigenetic Regulation of Inflammatory Cytokine-Induced Epithelial-To-Mesenchymal Cell Transition and Cancer Stem Cell Generation. Cells. 2019;8(10)doi:10.3390 /cells8101143
- 8. Rayburn ER, Ezell SJ, Zhang R. Anti-Inflammatory Agents for Cancer Therapy. Mol Cell Pharmacol. 2009;1(1):29-43. doi:10.4255/mcpharmacol.09.05
- 9. Delgado-Enciso I, Soriano-Hernandez AD, RodriguezHernandez A, et al. Histological changes caused by meclofenamic acid in androgen-independent prostate cancer tumors: evaluation in a mouse model. Int Braz J Urol. 2015;41(5):1002-7. doi:10.1590/S1677-5538.IBJU .2013.00186
- 10. Soriano-Hernandez AD, Madrigal-Perez D, Galvan-Salazar HR, et al. Anti-inflammatory drugs and uterine cervical cancer cells: Antineoplastic effect of meclofenamic acid. Oncol Lett. 2015;10(4):2574-2578. doi:10.3892/ol.2015.3 580
- 11. Cui Q, Shi H, Ye P, et al. m(6)A RNA Methylation Regulates the Self-Renewal and Tumorigenesis of Glioblastoma Stem Cells. Cell Rep. 2017;18(11):2622-2634. doi:10.1016/j. celrep.2017.02.059
- 12. Sekine Y, Nakayama H, Miyazawa Y, et al. Simvastatin in combination with meclofenamic acid inhibits the proliferation and migration of human prostate cancer PC3 cells via an AKR1C3 mechanism. Oncol Lett. 2018;15(3):3167-3172. doi:10.3892/ol.2017.7721
- 13. Wen L, Pan X, Yu Y, Yang B. Down-regulation of FTO promotes proliferation and migration, and protects bladder cancer cells from cisplatin-induced cytotoxicity. BMC Urol. 2020;20(1):39. doi:10.1186/s12894-020-00612- 7
- 14. Image Progecessing and Analysis in Java (ImageJ). https://imagej.nih.gov/ij/
- 15. Kanli A, Kasap, M., Akpinar, G., Yanar, S. . Changes occurring in the proteome of SH-SY5Y cells caused by fat mass and obesity assocıated (FTO) protein expression reveals multifaceted properties of the FTO protein. . Journal of Health Sciences of Kocaeli University. 2020;6(2):101-112.
- 16. Ahmed S, Alam W, Jeandet P, et al. Therapeutic Potential of Marine Peptides in Prostate Cancer: Mechanistic Insights. Mar Drugs. 2022;20(8)doi:10.3390/md20080466
- 17. Siddiqui IA, Malik A, Adhami VM, et al. Green tea polyphenol EGCG sensitizes human prostate carcinoma LNCaP cells to TRAIL-mediated apoptosis and synergistically inhibits biomarkers associated with angiogenesis and metastasis. Oncogene. 2008;27 (14):2055-63. doi:10.1038/sj.onc.1210840
- 18. Koshida K, Konaka H, Imao T, Egawa M, Mizokami A, Namiki M. Comparison of two in vivo models for prostate cancer: orthotopic and intratesticular inoculation of LNCaP or PC-3 cells. Int J Urol. 2004;11(12):1114-21. doi:10.1111/j.1442-2042.2004.00961.x
- 19. Koeneman KS, Yeung F, Chung LW. Osteomimetic properties of prostate cancer cells: a hypothesis supporting the predilection of prostate cancer metastasis and growth in the bone environment. Prostate. 1999;39(4):246-61. doi:10.1002/(sici)1097-0045(199906 01)39:4<246::aid-pros5>3.0.co;2-u
- 20. Chen H, Jia B, Zhang Q, Zhang Y. Meclofenamic Acid Restores Gefinitib Sensitivity by Downregulating Breast Cancer Resistance Protein and Multidrug Resistance Protein 7 via FTO/m6A-Demethylation/c-Myc in Non-Small Cell Lung Cancer. Front Oncol. 2022;12:870636. doi:10.3389/fonc.2022.870636
- 21. Sahinoz B, Kanli, A. Meclofenamic Acid, a Pharmacological Agent, Regulates the m6A Level by Inhibition the FTO Protein in Prostate Cancer Cell Line LNCaP Cells. IKSAD GLOBAL PUBLISHING HOUSE. 2021:195-204.
- 22. Araldi EM, Dell'aica I, Sogno I, Lorusso G, Garbisa S, Albini A. Natural and synthetic agents targeting inflammation and angiogenesis for chemoprevention of prostate cancer. Curr Cancer Drug Targets. 2008;8(2):146-55. doi:10.217 4/156800908783769382
- 23. Albini A, Tosetti F, Li VW, Noonan DM, Li WW. Cancer prevention by targeting angiogenesis. Nat Rev Clin Oncol. 2012;9(9):498-509. doi:10.1038/nrclinonc.2012.120
- 24. Moroz A, Delella FK, Almeida R, et al. Finasteride inhibits human prostate cancer cell invasion through MMP2 and MMP9 downregulation. PLoS One. 2013;8(12):e84757. doi:10.1371/journal.pone.0084757
- 25. Lee SH, Hatakeyama S, Yu SY, et al. Core3 O-glycan synthase suppresses tumor formation and metastasis of prostate carcinoma PC3 and LNCaP cells through downregulation of alpha2beta1 integrin complex. J Biol Chem. 2009;284(25):17157-17169. doi:10.1074/jbc.M109 .010934
- 26. Shin DY, Lee WS, Jung JH, et al. Flavonoids from Orostachys japonicus A. Berger inhibit the invasion of LnCaP prostate carcinoma cells by inactivating Akt and modulating tight junctions. Int J Mol Sci. 2013;14(9):18407-20. doi:10.339 0/ijms140918407
- 27. Soriano-Hernandez AD, Galvan-Salazar HR, MontesGalindo DA, et al. Antitumor effect of meclofenamic acid on human androgen-independent prostate cancer: a preclinical evaluation. Int Urol Nephrol. 2012;44(2):471-7. doi:10.1007/s11255-011-0012-0
- 28. Guzman-Esquivel J, Mendoza-Hernandez MA, TiburcioJimenez D, et al. Decreased biochemical progression in patients with castration-resistant prostate cancer using a novel mefenamic acid anti-inflammatory therapy: A randomized controlled trial. Oncol Lett. 2020;19(6):4151- 4160. doi:10.3892/ol.2020.11509
- 29. Kokkinos MI, Wafai R, Wong MK, Newgreen DF, Thompson EW, Waltham M. Vimentin and epithelial-mesenchymal transition in human breast cancer--observations in vitro and in vivo. Cells Tissues Organs. 2007;185(1-3):191-203. doi:10.1159/000101320
- 30. Zhao Y, Yan Q, Long X, Chen X, Wang Y. Vimentin affects the mobility and invasiveness of prostate cancer cells. Cell Biochem Funct. 2008;26(5):571-7. doi:10.1002/cbf.1478
- 31. Lang SH, Hyde C, Reid IN, et al. Enhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma. Prostate. 2002;52(4):253-63. doi:10.1002/pros.10088
| APA | KANLI A, Yanar S (2022). THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. , 142 - 147. 10.53446/actamednicomedia.1166837 |
| Chicago | KANLI Aylin,Yanar Sevinc THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. (2022): 142 - 147. 10.53446/actamednicomedia.1166837 |
| MLA | KANLI Aylin,Yanar Sevinc THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. , 2022, ss.142 - 147. 10.53446/actamednicomedia.1166837 |
| AMA | KANLI A,Yanar S THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. . 2022; 142 - 147. 10.53446/actamednicomedia.1166837 |
| Vancouver | KANLI A,Yanar S THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. . 2022; 142 - 147. 10.53446/actamednicomedia.1166837 |
| IEEE | KANLI A,Yanar S "THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS." , ss.142 - 147, 2022. 10.53446/actamednicomedia.1166837 |
| ISNAD | KANLI, Aylin - Yanar, Sevinc. "THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS". (2022), 142-147. https://doi.org/10.53446/actamednicomedia.1166837 |
| APA | KANLI A, Yanar S (2022). THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. Acta Medica Nicomedia, 5(3), 142 - 147. 10.53446/actamednicomedia.1166837 |
| Chicago | KANLI Aylin,Yanar Sevinc THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. Acta Medica Nicomedia 5, no.3 (2022): 142 - 147. 10.53446/actamednicomedia.1166837 |
| MLA | KANLI Aylin,Yanar Sevinc THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. Acta Medica Nicomedia, vol.5, no.3, 2022, ss.142 - 147. 10.53446/actamednicomedia.1166837 |
| AMA | KANLI A,Yanar S THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. Acta Medica Nicomedia. 2022; 5(3): 142 - 147. 10.53446/actamednicomedia.1166837 |
| Vancouver | KANLI A,Yanar S THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS. Acta Medica Nicomedia. 2022; 5(3): 142 - 147. 10.53446/actamednicomedia.1166837 |
| IEEE | KANLI A,Yanar S "THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS." Acta Medica Nicomedia, 5, ss.142 - 147, 2022. 10.53446/actamednicomedia.1166837 |
| ISNAD | KANLI, Aylin - Yanar, Sevinc. "THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS". Acta Medica Nicomedia 5/3 (2022), 142-147. https://doi.org/10.53446/actamednicomedia.1166837 |
