TY  - JOUR
TI  - THE EFFECT OF MECLOFENAMIC ACID ON THE INVASION AND MIGRATION OF LNCaP PROSTATE CARCINOMA CELLS
AB  - Objective: The treatment of prostate cancer has not significantly advanced despite several research to enhance early diagnosis and the introduction of innovative therapy agents. Prostate cancer metastasis is also a significant obstacle to treatment approaches. Therefore, the aim of the current investigation was to examine how the non-steroidal anti-inflammatory medicine meclofenamic acid (MA) affected the invasion and migration of LNCaP prostate cancer cells. Methods: Firstly, the non-toxic concentrations of MA on LNCaP cells were determined by trypan blue exclusion assay. After that, the effect of MA on migration and invasion was evaluated by wound healing assay and matrigel invasion assay, respectively. Finally, western blotting was used to determine the vimentin expression level, a marker for the transition of epithelial to mesenchymal cells. Results: The results of trypan blue exclusion assay showed that 60 and 80 µM concentrations of MA were non-toxic to the cells. The migration rate of non-treated control cells was 44.4%, while it was significantly reduced to 12.9% and 2.9% in 60 and 80 µM MAtreated groups, respectively. When the cells were treated with 80 µM MA, it significantly reduced the invasion of LNCaP cells from 52% to 30.3%. Vimentin expression was considerably decreased in MA-treated cells, according to the results of a western blot. Conclusion: This study demonstrates for the first time that MA inhibits the migration and invasion of prostate cancer LNCaP cells by decreasing the expression level of vimentin.
AU  - KANLI, Aylin
AU  - Yanar, Sevinc
DO  - 10.53446/actamednicomedia.1166837
PY  - 2022
JO  - Acta Medica Nicomedia
VL  - 5
IS  - 3
SN  - 2717-8994
SP  - 142
EP  - 147
DB  - TRDizin
UR  - http://search/yayin/detay/1142024
ER  -