Yıl: 2011 Cilt: 35 Sayı: 2 Sayfa Aralığı: 125 - 136 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives

Öz:
Solanum cinsi içerisinde, ‘patlıcan’ terimi gıda ve daha az ölçüde tıp için kullanılan çeşitli kültür türlerini kapsamaktadır. Birden fazla türü tanımlamak için yaygın bir ismin kullanımı ve çok sayıda akraba yabani türlerin varlığı taksonomik karışıklığa yol açmıştır. Bu durumun bir sonucu olarak bu gruplar içerisindeki türlerin evrimsel ilişkilerin analizleri ve genetik çeşitliliğin ortaya çıkarılması karmaşık hale gelmiştir. Patlıcan araştırmaları için ortaya çıkan diğer bir sorun da, aslında fi logenetik çalışmalar için moleküler işaretleyicilerin kullanımı iyi kurulmasına rağmen, çok az sayıdaki çalışmada patlıcan için yeni işaretleyicilerin geliştirilmesi nitelendirilmiştir. Bu çalışmada, genik mikrosatalit (SSR) işaretleri Solanum melongena türü için geliştirilmiş bir ifade edilmiş DNA dizi etiketi kütüphanesinden belirlenmiştir ve 47 adet patlıcan ve yakın akraba türlerine ait tohum örneğinin analizinde kullanılmıştır. Bu işaretleyiciler aralarında 8 adet Solanum melongena tohum örneği içeren 18 türde çok iyi polimorfi zm vermiştir. Ayrıca, bu işaretleyiciler kullanılarak yapılan genetik analizler önceki araştırmalarla ve patlıcanın ıslahı ile ilgili bilinen bilgiler ile uyum göstermiştir. Bu işaretleyicilerin patlıcan da genetik ilişkilerin belirlenmesi, parmakizi analizleri ve genetik haritalama çalışmaları için çok değerli kaynak oluşturmaları beklenmektedir.
Anahtar Kelime: Solanum mikrosatellitler Solanum aethiopicum patlıcan Solanum melongena Solanum macrocarpon genetik farklılık SSR

Konular: Biyoloji

Patlıcan ve yakın akraba türlerinde genetik çeşitliliği incelemek için EST-SSRs işaretleyicilerinin uygulanması

Öz:
Within the genus Solanum, the term ‘eggplant’ encompasses several cultivated species that are used for food and, to a lesser extent, for medicine. Th e use of one common name to describe more than one species and the existence of many related wild species have led to taxonomic confusion which, in turn, have complicated analyses of evolutionary relationships and genetic diversity within this groups of species. A further challenge for eggplant research is that, despite the fact that the use of molecular markers for phylogenetic studies is well-established, very few studies have described the development of new markers for eggplant. In our work, genic microsatellite (SSR) markers were identifi ed from an expressed sequence tag library of S. melongena and used for analysis of 47 accessions of eggplant and closely related species. Th e markers had very good polymorphism in the 18 species tested including 8 S. melongena accessions. Moreover, genetic analysis performed with these markers showed concordance with previous research and knowledge of eggplant domestication. Th ese markers are expected to be a valuable resource for studies of genetic relationships, fi ngerprinting, and gene mapping in eggplant.
Anahtar Kelime: Solanum melongena Solanum macrocarpon genetic diversity SSR Solanum microsatellites Solanum aethiopicum aubergines

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA TÜMBİLEN y, Frary A, DAUNAY M, Doganlar S (2011). Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. , 125 - 136.
Chicago TÜMBİLEN yeliz,Frary Anne,DAUNAY Marie Christine,Doganlar Sami Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. (2011): 125 - 136.
MLA TÜMBİLEN yeliz,Frary Anne,DAUNAY Marie Christine,Doganlar Sami Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. , 2011, ss.125 - 136.
AMA TÜMBİLEN y,Frary A,DAUNAY M,Doganlar S Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. . 2011; 125 - 136.
Vancouver TÜMBİLEN y,Frary A,DAUNAY M,Doganlar S Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. . 2011; 125 - 136.
IEEE TÜMBİLEN y,Frary A,DAUNAY M,Doganlar S "Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives." , ss.125 - 136, 2011.
ISNAD TÜMBİLEN, yeliz vd. "Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives". (2011), 125-136.
APA TÜMBİLEN y, Frary A, DAUNAY M, Doganlar S (2011). Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. Turkish Journal of Biology, 35(2), 125 - 136.
Chicago TÜMBİLEN yeliz,Frary Anne,DAUNAY Marie Christine,Doganlar Sami Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. Turkish Journal of Biology 35, no.2 (2011): 125 - 136.
MLA TÜMBİLEN yeliz,Frary Anne,DAUNAY Marie Christine,Doganlar Sami Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. Turkish Journal of Biology, vol.35, no.2, 2011, ss.125 - 136.
AMA TÜMBİLEN y,Frary A,DAUNAY M,Doganlar S Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. Turkish Journal of Biology. 2011; 35(2): 125 - 136.
Vancouver TÜMBİLEN y,Frary A,DAUNAY M,Doganlar S Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. Turkish Journal of Biology. 2011; 35(2): 125 - 136.
IEEE TÜMBİLEN y,Frary A,DAUNAY M,Doganlar S "Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives." Turkish Journal of Biology, 35, ss.125 - 136, 2011.
ISNAD TÜMBİLEN, yeliz vd. "Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives". Turkish Journal of Biology 35/2 (2011), 125-136.