TY  - JOUR
TI  - EGFR blocker lapatinib inhibits the synthesis of matrix metalloproteinases from synovial fibroblasts
AB  - Background/aim: Epidermal growth factor receptor (EGFR) family members and their associated ligands may be related to bone and joint destruction in rheumatoid arthritis. Matrix metalloproteinases are responsible for joint and bone tissue degradation. This study is intended to investigate the effect of epidermal growth factor receptor inhibition by lapatinib on the synthesis of matrix metalloproteinases in in vitro. Materials and methods: Synovial fibroblast cell culture was obtained from a patient with rheumatoid arthritis who underwent knee arthroplasty. Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were added to the cell culture to stimulate synovial fibroblast cells and create an inflammatory character. Understimulated and nonstimulated conditions, lapatinib was applied to the culture in four different concentrations of 25, 50, 100, and 200 μmol. Then, matrix metalloproteinase -1, -3, and, -13 levels were assessed. Results: When stimulated with IL-1β and TNF-α, the synthesis of matrix metalloproteinases from synovial fibroblast was increased significantly. When lapatinib is added to the stimulated synovial fibroblasts, matrix metalloproteinases synthesis is significantly suppressed. Conclusion: Inhibition of the EGFR pathway with lapatinib suppresses matrix metalloproteinases synthesis. Our results suggest EGFR pathway inhibition may be a promising option to prevent joint destruction in the treatment of rheumatoid arthritis.
AU  - Ozgen, Metin
AU  - Yalcin Kehribar, Demet
AU  - emmungil, hakan
AU  - Salva, Emine
AU  - başak türkmen, neşe
AU  - ÇİFTÇİ, OSMAN
DO  - 10.55730/1300-0144.5442
PY  - 2022
JO  - Turkish Journal of Medical Sciences
VL  - 52
IS  - 4
SN  - 1300-0144
SP  - 1355
EP  - 1361
DB  - TRDizin
UR  - http://search/yayin/detay/1144909
ER  -