Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells

Merve ÇAPKIN YURTSEVER, (Adana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, Adana, Türkiye)
Özge Ekin AKDERE, (Hacettepe Üniversitesi, Fen Bilimleri Enstitüsü, Biyomühendislik Bölümü, Ankara, Türkiye)
Menemşe GÜMÜŞDERELİOĞLU (Hacettepe Üniversitesi, Fen Bilimleri Enstitüsü, Biyomühendislik Bölümü, Ankara, Türkiye)
Turkish Journal of Biology
2 0
Yıl: 2022 Cilt: 46 Sayı: 6 Sayfa Aralığı: 475 - 487 Metin Dili: İngilizce DOI: 10.55730/1300-0152.2633 İndeks Tarihi: 30-12-2022

Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells

Öz:
Chitosan has high biocompatibility, supports proliferation of many cells, and can be a good carrier for various growth factors. However, low attachment ratio and spheroid formation of several stem cell types on plain chitosan scaffolds/films is still a problem. In this study, it was aimed to obtain 3D scaffolds using medical grade chitosan (MC) with a high deacetylation degree (DD ≥ 92.6%) to overcome the spheroid formation of rat adipose tissue derived mesenchymal stem cells (rAdMSCs) on control chitosan (C, DD = 75%–85%) scaffolds. Genipin was used as a biological chemical crosslinker, and glycerol phosphate salt was used both as a pH adjusting agent and physical crosslinker. MTT and SEM analyses and live/dead staining indicated the increase in the attachment, cell viability, and proliferation of rAdMSCs on MC scaffolds with or without crosslinking when compared to the cells in spheroid formation on control scaffolds. Moreover, filamentous actin protein organization of rAdMSCs was found to be triggered on the crosslinked MC scaffolds. In conclusion, plain medical grade chitosan scaffolds with or without crosslinking prevented spheroid formation, supported the attachment, proliferation, and organization of rAdMSCs indicating that medical grade type of chitosan scaffolds with high DD can be a very good candidate as 3D carriers in stem cell cultivation.
Anahtar Kelime: Medical grade chitosan genipin stem cell scaffold

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  • Ahmed S, Annu AA, Sheikh J (2018). A review on chitosan centred scaffolds and their applications in tissue engineering. International Journal of Biological Macromolecules 116: 849– 862. https://doi.org/10.1016/j.ijbiomac.2018.04.176
  • Akdere ÖE, Shikhaliyeva İ, Gümüşderelioğlu M (2019). Boron mediated 2D and 3D cultures of adipose derived mesenchymal stem cells. Cytotechnology 9. https://doi.org/10.1007/s10616- 019-00310-9
  • Asghari Sana F, Çapkın Yurtsever M, Kaynak Bayrak G, Tunçay EÖ, Kiremitçi AS et al. (2017). Spreading, proliferation and differentiation of human dental pulp stem cells on chitosan scaffolds immobilized with RGD or fibronectin. Cytotechnology 69: 617–630. https://doi.org/10.1007/s10616- 017-0072-9
  • Baraniak PR, Cooke MT, Saeed R, Kinney MA, Fridley KM et al. (2012). Stiffening of human mesenchymal stem cell spheroid microenvironments induced by incorporation of gelatin microparticles. Journal of the Mechanical Behavior of Biomedical Materials 11: 63–71. https://doi.org/10.1016/j. jmbbm.2012.02.018
  • Bezrodnykh EA, Vyshivannaya OV, Polezhaev AV, Abramchuk SS, Blagodatskikh IV et al. (2020). Residual heavy metals in industrial chitosan: State of distribution. International Journal of Biological Macromolecules 155: 979–986. https://doi. org/10.1016/j.ijbiomac.2019.11.059
  • Bush JR, Liang H, Dickinson M, Botchwey EA (2016). Xylan hemicellulose improves chitosan hydrogel for bone tissue regeneration. Polymers for Advanced Technologies 27: 1050– 1055. https://doi.org/10.1002/pat.3767
  • Busilacchi A, Gigante A, Mattioli-Belmonte M, Manzotti S, RAA Muzzarelli (2013). Chitosan stabilizes platelet growth factors and modulates stem cell differentiation toward tissue regeneration. Carbohydrate Polymers 98: 665–676. https://doi. org/10.1016/j.carbpol.2013.06.044
  • Cesarz Z, Tamama K (2016). Spheroid Culture of Mesenchymal Stem Cells. Stem Cells International 2016. https://doi. org/10.1155/2016/9176357
  • Cheng NC, Wang S, Young TH (2012). The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities. Biomaterials 33: 1748–1758. https://doi.org/10.1016/j. biomaterials.2011.11.049
  • Chou SF, Lai JY, Cho CH, Lee CH (2016). Relationships between surface roughness/stiffness of chitosan coatings and fabrication of corneal keratocyte spheroids: Effect of degree of deacetylation. Colloids and Surfaces B: Biointerfaces 142: 105–113. https://doi.org/10.1016/j.colsurfb.2016.02.051
  • Das P, Salerno S, Remigy JC, Lahitte JF, Bacchin P et al. (2019). Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells. Colloids and Surfaces B: Biointerfaces 184. https://doi.org/10.1016/j. colsurfb.2019.110493
  • Dawlee S, Sugandhi A, Balakrishnan B, Labarre D, Jayakrishnan A (2005). Oxidized chondroitin sulfate-cross-linked gelatin matrixes: A new class of hydrogels. Biomacromolecules 6: 2040–2048. https://doi.org/10.1021/bm050013a
  • Ginzberg MB, Kafri R, Kirschner M (2015). On being the right (cell) size. Science 348: 1245075. https://doi.org/10.1126/ science.1245075
  • Gültan T, Bektaş Tercan Ş, Çetin Altındal D , Gümüşderelioğlu M (2021). Synergistic effect of fabrication and stabilization methods on physicochemical and biological properties of chitosan scaffolds. International Journal of Polymeric Materials and Polymeric Biomaterials 70 (6): 371-382. https://doi.org/10 .1080/00914037.2020.1725752
  • He J, Wu F, Wang D, Yao R, Wu Y et al. (2015). Modulation of cationicity of chitosan for tuning mesenchymal stem cell adhesion, proliferation, and differentiation. Biointerphases 10: 04A304. https://doi.org/10.1116/1.4932379
  • Hsu SH, Ho TT, Huang NC, Yao CL, Peng LH et al. (2014). Substrate- dependent modulation of 3D spheroid morphology self- assembled in mesenchymal stem cell-endothelial progenitor cell coculture. Biomaterials 35: 7295–7307. https://doi. org/10.1016/j.biomaterials.2014.05.033
  • Hsu S, Whu SW, Tsai C-L, Wu Y-H, Chen H-W et al. (2004). Chitosan as Scaffold Materials: Effects of Molecular Weight and Degree of Deacetylation. Journal of Polymer Research 11: 141–147. https://doi.org/10.1023/B:JPOL.0000031080.70010.0b
  • Huang GS, Dai LG, Yen BL, Hsu SH (2011). Spheroid formation of mesenchymal stem cells on chitosan and chitosan-hyaluronan membranes. Biomaterials 32: 6929–6945. https://doi. org/10.1016/j.biomaterials.2011.05.092
  • Ikeda T, Ikeda K, Yamamoto K, Ishizaki H, Yoshizawa Y et al. (2014). Fabrication and characteristics of chitosan sponge as a tissue engineering scaffold. BioMed Research International 2014. https://doi.org/10.1155/2014/786892
  • Jeong K-H, Park D, Lee Y-C (2017). Polymer-based hydrogel scaffolds for skin tissue engineering applications: a mini-review. Journal of Polymer Research 24: 112. https://doi.org/10.1007/s10965- 017-1278-4
  • Jhala D, Rather HA, R Vasita (2020). Extracellular matrix mimicking polycaprolactone-chitosan nanofibers promote stemness maintenance of mesenchymal stem cells via spheroid formation. Biomedical Materials 15: 35011. https://doi. org/10.1088/1748-605x/ab772e
  • Kafi MA, Aktar MK, Phanny Y, Todo M (2019). Adhesion, proliferation and differentiation of human mesenchymal stem cell on chitosan/collagen composite scaffold. Journal of Materials Science: Materials in Medicine 30: 131. https://doi. org/10.1007/s10856-019-6341-8
  • Kurita K, Kaji Y, Mori T, Nishiyama Y (2000). Enzymatic degradation of β-chitin: susceptibility and the influence of deacetylation. Carbohydrate Polymers 42: 19–21. https://doi.org/https://doi. org/10.1016/S0144-8617(99)00127-7
  • Lotfi M, Naderi-Meshkin H, Mahdipour E, Mafinezhad A, Bagherzadeh R et al. (2019). Adipose tissue-derived mesenchymal stem cells and keratinocytes co-culture on gelatin/chitosan/β-glycerol phosphate nanoscaffold in skin regeneration. Cell Biology International. https://doi. org/10.1002/cbin.11119
  • Murphy CM, Haugh MG, O’Brien FJ (2010). The effect of mean pore size on cell attachment, proliferation and migration in collagen- glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials 31: 461–466. https://doi.org/10.1016/j. biomaterials.2009.09.063
  • Muzzarelli RAA (2009). Genipin-crosslinked chitosan hydrogels as biomedical and pharmaceutical aids. Carbohydrate Polymers 77: 1–9. https://doi.org/10.1016/j.carbpol.2009.01.016
  • Muzzarelli RAA, Mehtedi ME, Bottegoni C, Aquili A, Gigante A (2015). Genipin-crosslinked chitosan gels and scaffolds for tissue engineering and regeneration of cartilage and bone. Marine Drugs 13: 7314–7338. https://doi.org/10.3390/ md13127068
  • Muzzarelli RAA, Mehtedi ME, Bottegoni C, Gigante A (2016). Physical properties imparted by genipin to chitosan for tissue regeneration with human stem cells: A review. International Journal of Biological Macromolecules 93: 1366–1381. https:// doi.org/10.1016/j.ijbiomac.2016.03.075
  • Pandey AR, Singh US, Momin M, Bhavsar C (2017). Chitosan: Application in tissue engineering and skin grafting. Journal of Polymer Research 24: 125. https://doi.org/10.1007/s10965- 017-1286-4
  • Petrova VA, Chernyakov DD, Poshina DN, Gofman IV, Romanov DP et al. (2019). Electrospun bilayer chitosan/hyaluronan material and its compatibility with mesenchymal stem cells. Materials 12: 1–17. https://doi.org/10.3390/ma12122016
  • Queiroz MF, Melo KR, Sabry DA, Sassaki GL, Rocha HA (2014) Does the use of chitosan contribute to oxalate kidney stone formation? Marine Drugs 13 (1):141-58. https://doi. org/10.3390/md13010141
  • Reys LL, Silva SS, Pirraco RP, Marques AP, Mano JF et al. (2017). Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering. European Polymer Journal 95: 232–240. https://doi.org/10.1016/j.eurpolymj.2017.08.017
  • Ronchi G, Fornasari BE, Crosio A, Budau CA, Tos P et al. (2018). Chitosan Tubes Enriched with Fresh Skeletal Muscle Fibers for Primary Nerve Repair. BioMed Research International 2018: 9175248. https://doi.org/10.1155/2018/9175248
  • Santos E, Hernández RM, Pedraz JL, Orive G (2012). Novel advances in the design of three-dimensional bio-scaffolds to control cell fate: Translation from 2D to 3D. Trends in Biotechnology 30: 331–341. https://doi.org/10.1016/j.tibtech.2012.03.005
  • Skwarczynska A, Kaminska M, Owczarz P, Bartoszek N, Walkowiak B et al. (2018) The structural (FTIR, XRD, and XPS) and biological studies of thermosensitive chitosan chloride gels with b-glycerophosphate disodium. Journal of Applied Polymer Science 46459: 1-6. https://doi.org/10.1002/app.46459
  • Stenberg L, Kodama A, Lindwall-Blom C, Dahlin LB (2016). Nerve regeneration in chitosan conduits and in autologous nerve grafts in healthy and in type 2 diabetic Goto-Kakizaki rats. European Journal of Neuroscience 43: 463–473. https://doi. org/10.1111/ejn.13068
  • Stie MB, Jones M, Sørensen HO, Jacobsen J, Chronakis IS et al. (2019). Acids ‘generally recognized as safe’ affect morphology and biocompatibility of electrospun chitosan/polyethylene oxide nanofibers. Carbohydrate Polymers 215: 253–262. https://doi.org/10.1016/j.carbpol.2019.03.061
  • Tığlı RS, Karakeçili A, Gümüşderelioğlu M (2007). In vitro characterization of chitosan scaffolds: influence of composition and deacetylation degree. Journal of Materials Science. Materials in Medicine 18: 1665–1674. https://doi.org/10.1007/ s10856-007-3066-x
  • Yan XZ, van den Beucken JJJP, Yuan C, Jansen JA, Yang F (2018). Spheroid formation and stemness preservation of human periodontal ligament cells on chitosan films. Oral Diseases 24: 1083–1092. https://doi.org/10.1111/odi.12855
  • Yeh HY, Liu BH, Hsu SH (2012). The calcium-dependent regulation of spheroid formation and cardiomyogenic differentiation for MSCs on chitosan membranes. Biomaterials 33: 8943–8954. https://doi.org/10.1016/j.biomaterials.2012.08.069
  • Yeh HY, Liu BH, Sieber M, Hsu SH (2014). Substrate-dependent gene regulation of self-assembled human MSC spheroids on chitosan membranes. BMC Genomics 15. https://doi. org/10.1186/1471-2164-15-10
  • Zhang S, Liu P, Chen L, Wang Y, Wang Z et al. (2015). The effects of spheroid formation of adipose-derived stem cells in a microgravity bioreactor on stemness properties and therapeutic potential. Biomaterials 41: 15–25. https://doi.org/10.1016/j. biomaterials.2014.11.019
APA CAPKIN YURTSEVER M, Akdere Ö, Gümüşderelioğlu M (2022). Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. , 475 - 487. 10.55730/1300-0152.2633
Chicago CAPKIN YURTSEVER MERVE,Akdere Özge Ekin,Gümüşderelioğlu Menemşe Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. (2022): 475 - 487. 10.55730/1300-0152.2633
MLA CAPKIN YURTSEVER MERVE,Akdere Özge Ekin,Gümüşderelioğlu Menemşe Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. , 2022, ss.475 - 487. 10.55730/1300-0152.2633
AMA CAPKIN YURTSEVER M,Akdere Ö,Gümüşderelioğlu M Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. . 2022; 475 - 487. 10.55730/1300-0152.2633
Vancouver CAPKIN YURTSEVER M,Akdere Ö,Gümüşderelioğlu M Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. . 2022; 475 - 487. 10.55730/1300-0152.2633
IEEE CAPKIN YURTSEVER M,Akdere Ö,Gümüşderelioğlu M "Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells." , ss.475 - 487, 2022. 10.55730/1300-0152.2633
ISNAD CAPKIN YURTSEVER, MERVE vd. "Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells". (2022), 475-487. https://doi.org/10.55730/1300-0152.2633
APA CAPKIN YURTSEVER M, Akdere Ö, Gümüşderelioğlu M (2022). Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. Turkish Journal of Biology, 46(6), 475 - 487. 10.55730/1300-0152.2633
Chicago CAPKIN YURTSEVER MERVE,Akdere Özge Ekin,Gümüşderelioğlu Menemşe Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. Turkish Journal of Biology 46, no.6 (2022): 475 - 487. 10.55730/1300-0152.2633
MLA CAPKIN YURTSEVER MERVE,Akdere Özge Ekin,Gümüşderelioğlu Menemşe Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. Turkish Journal of Biology, vol.46, no.6, 2022, ss.475 - 487. 10.55730/1300-0152.2633
AMA CAPKIN YURTSEVER M,Akdere Ö,Gümüşderelioğlu M Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. Turkish Journal of Biology. 2022; 46(6): 475 - 487. 10.55730/1300-0152.2633
Vancouver CAPKIN YURTSEVER M,Akdere Ö,Gümüşderelioğlu M Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells. Turkish Journal of Biology. 2022; 46(6): 475 - 487. 10.55730/1300-0152.2633
IEEE CAPKIN YURTSEVER M,Akdere Ö,Gümüşderelioğlu M "Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells." Turkish Journal of Biology, 46, ss.475 - 487, 2022. 10.55730/1300-0152.2633
ISNAD CAPKIN YURTSEVER, MERVE vd. "Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells". Turkish Journal of Biology 46/6 (2022), 475-487. https://doi.org/10.55730/1300-0152.2633
Sayfa Oluşturulma Tarihi
24-04-2024 5:7

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