Salmonellosis caused by nontyphoidal Salmonella is the second most common zoonotic infection worldwide. The major foodborne route of this infection is the consumption of contaminated poultry meat and egg. Therefore, monitoring and control of Salmonella agents in poultry populations is critical to reduce the risks for public health. In the poultry industry, the most common serotypes in Salmonella infections are Salmonella enterica subsp. enterica serotype Enteritidis and Typhimurium. In recent years, other frequently isolated Salmonella serotypes have also increased in Turkey because of the Salmonella control programs regulated by many countries. Hence, other Salmonella serotypes (except for the most frequently isolated serotypes, S. Enteritidis, S. Typhimurium) may become dominant in the future, so their epidemiology needs to be investigated. In this study, Multilocus Sequence Typing (MLST) was used to type rarely found Salmonella serotypes by sequencing seven housekeeping genes for each strain. The broiler chicken originated 54 Salmonella strains were used as Salmonella Bazenheid (n = 4), Salmonella Burgas (n = 5), Salmonella Gombe (n = 8), Salmonella Hayindogo (n = 9), Salmonella Jamaica (n = 4), Salmonella Kamoru (n = 10), Salmonella Paris (n = 2), and Salmonella Stuttgart (n = 12). This study is the first study to use MLST to serotype S. Bazenheid (sequence type, ST544), S. Burgas (ST1814), S. Gombe (ST413), S. Hayindogo (ST1959), S. Jamaica (ST32), S. Kamoru (ST1995), S. Paris (ST82), and S. Stuttgart (ST19) in our country. The presence of a single sequence type for each serotype suggests that these serotypes may be the dominant sequence types in broiler chicken flocks in Turkey.