TY  - JOUR
TI  - Ganoderma lucidum inhibits proliferation and promotes autophagy in breast cancer cell lines with varying hormonal sensitivity by regulating Beclin-1, LC3, and p62
AB  - Purpose: The aim of this study was to investigate the role of Ganoderma lucidum (GL) in cell proliferation and autophagy in breast cancer cell lines. Materials and Methods: Cell lines were cultured in vitro and the appropriate dose was determined by the MTT method. In order to show the effect of GL on autophagy in MCF-7 and MDA-MB-231 breast cancer cell lines, Beclin-1, LC3 and p62 expression were examined by immunohistochemistry staining. Additionally, cell cycle analysis was carried out in GL-treated and untreated cells using a cell cycle analysis kit to look into how GL affects cell division. Results: Viability level was significantly reduced in MCF-7 and MDA-MB-231 cells incubated with 40 and 80 μM concentrations of GL for 24 hours. In addition, GL induced the initiation of autophagy in MDA-MB-231 and MCF-7 cells, as evidenced by enhanced levels of Beclin-1, LC3 and p62 expression. At the same time, this study showed that GL induced cell cycle arrest in the G2/M phases. Conclusion: GL has anti-proliferative activity by arresting the cell cycle at the G2/M phase and accelerates cell death by inducing autophagy, particularly in aggressive breast cancers that are resistant to treatment..
AU  - yakan, birkan
AU  - Göktepe, Özge
AU  - Önder, Gözde Özge
AU  - ÇINAR, Venhar
AU  - Yay, Arzu Hanım
AU  - Hamurcu, Zuhal
AU  - Bolat, Demet
DO  - 10.17826/cumj.1182496
PY  - 2022
JO  - Cukurova Medical Journal
VL  - 47
IS  - 4
SN  - 2602-3032
SP  - 1620
EP  - 1629
DB  - TRDizin
UR  - http://search/yayin/detay/1149698
ER  -