This study was carried out between 2017-2018 and the effect of cutting collecting time and IBA doses on root and shoot growth and development of goji berry (Lycium barbarum L.) cuttings were determined. Cuttings collected three years old Damaye and NQ7 goji berry cultivars grown in Karkin Village of Aksaray Province. Semi-hardwood and hardwood cuttings collected at 4 different times (August-15, October-15, December-15 and February-15) and applied with 4 different Indole-3-butryic-acid (IBA) doses (0, 500, 1000 and 2000 ppm) in bottom heated trays with perlite medium and over misting under high tunnel. They planted in trays with perlite medium under misting and bottom heating for rooting. The study planned with three replications and 25 cuttings for each replication under randomized complete block design under greenhouse. Cuttings were mowed after two months and root length (cm), root number, rooting degree (1-9), shoot length (cm) and diameter (mm) were determined. According to the results of the experiment, the number of roots (10.80), root length (5.29 cm), rooting degree (6.39) and shoot diameter (1.48 mm) were the highest in August semi-hardwood cuttings from Damaye and with 1000 ppm IBA dose. In terms of the properties examined for root and shoot growth and development of goji berry cuttings, the best results were obtained from Damaye variety, while the best cutting taking time was August, semi-hardwood cuttings and the IBA dose was 1000 ppm.