Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract

GÜN GÖK, Zehra; YİĞİTOĞLU, Mustafa; Ekici, Hüsamettin; BOZKAYA, OGÜN; arat, esra; Ekici, Seda; Vargel, ibrahim

doi:10.33988/auvfd.1014802

Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract

Ogün BOZKAYA, (Hacettepe Üniversitesi, Fen Bilimleri Enstitüsü, Biyomühendislik Anabilim Dalı, Ankara, Türkiye)

Hüsamettin EKİCİ, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı , Kırıkkale, Türkiye)

Zehra GÜN GÖK, (Kırıkkale Üniversitesi, Mühendislik Mimarlık Fakültesi, Biyomühendislik Bölümü, Kırıkkale, Türkiye)

Esra ARAT, (Kırıkkale Üniversitesi, Bilimsel ve Teknolojik Araştırma Uygulama ve Araştırma Merkezi, Kırıkkale, Türkiye)

Seda EKİCİ, (Veteriner Kontrol Merkezi Araştırma Enstitüsü, Ankara, Türkiye)

Mustafa YİĞİTOĞLU, (Kırıkkale Üniversitesi, Mühendislik Mimarlık Fakültesi, Biyomühendislik Bölümü, Kırıkkale, Türkiye)

İbrahim VARGEL (Veteriner Kontrol Merkezi Araştırma Enstitüsü, Ankara, Türkiye)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2023 Cilt: 70 Sayı: 1 Sayfa Aralığı: 87 - 96 Metin Dili: İngilizce DOI: 10.33988/auvfd.1014802 İndeks Tarihi: 17-05-2023

Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract

Öz:

In recent years, researchers have shown an increased interest in using medicinal plants for the synthesis of silver nanoparticles (AgNPs) having various therapeutic properties. Centella asiatica (CA), a medicinal plant, has been used to treat minor burn wounds, psoriasis, and hypertrophic wounds among many other pathological conditions. The current study aimed to synthesize CA coated AgNPs (CA-AgNPs) with appropriate biocompatibility and various therapeutic properties, including antimicrobial and analgesic activities. The synthesized CA-AgNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, zeta potential measurements, and fourier transform infrared (FT-IR) spectroscopy. The formation of spherical CA-AgNPs was confirmed by a single surface plasmon resonance (SPR) peak emerging at 420 nm wavelength by UV-Vis. The average hydrodynamic diameter and zeta potential of the particles were found to be 29.5 nm and -24.5 mV, respectively. The FTIR analyses showed that the AgNPs were coated and stabilized by bioactive compounds from the CA extract. MTT cytotoxicity assay revealed that CAAgNPs at .1 mM concentrations exhibited biocompatibility for L929 fibroblast cells. The antimicrobial activity of CA-AgNPs was confirmed by significant inhibition of Staphylococcus aureus and Escherichia coli. In addition, the analgesic effect of CA-AgNPs was investigated for the first time in the literature by tail-flick and hot plate methods, and statistically significant results were obtained for both methods. Taken together, these results suggest that CAAgNPs can be used as an effective antibacterial and analgesic agent in a variety of biomedical applications, including coating wound dressings.�

Anahtar Kelime: Analgesic effect antibacterial effect Centella asiatica cytotoxicity silver nanoparticles

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BOZKAYA O, Ekici H, GÜN GÖK Z, arat e, Ekici S, YİĞİTOĞLU M, Vargel i (2023). Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. , 87 - 96. 10.33988/auvfd.1014802
Chicago	BOZKAYA OGÜN,Ekici Hüsamettin,GÜN GÖK Zehra,arat esra,Ekici Seda,YİĞİTOĞLU Mustafa,Vargel ibrahim Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. (2023): 87 - 96. 10.33988/auvfd.1014802
MLA	BOZKAYA OGÜN,Ekici Hüsamettin,GÜN GÖK Zehra,arat esra,Ekici Seda,YİĞİTOĞLU Mustafa,Vargel ibrahim Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. , 2023, ss.87 - 96. 10.33988/auvfd.1014802
AMA	BOZKAYA O,Ekici H,GÜN GÖK Z,arat e,Ekici S,YİĞİTOĞLU M,Vargel i Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. . 2023; 87 - 96. 10.33988/auvfd.1014802
Vancouver	BOZKAYA O,Ekici H,GÜN GÖK Z,arat e,Ekici S,YİĞİTOĞLU M,Vargel i Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. . 2023; 87 - 96. 10.33988/auvfd.1014802
IEEE	BOZKAYA O,Ekici H,GÜN GÖK Z,arat e,Ekici S,YİĞİTOĞLU M,Vargel i "Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract." , ss.87 - 96, 2023. 10.33988/auvfd.1014802
ISNAD	BOZKAYA, OGÜN vd. "Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract". (2023), 87-96. https://doi.org/10.33988/auvfd.1014802

APA	BOZKAYA O, Ekici H, GÜN GÖK Z, arat e, Ekici S, YİĞİTOĞLU M, Vargel i (2023). Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 70(1), 87 - 96. 10.33988/auvfd.1014802
Chicago	BOZKAYA OGÜN,Ekici Hüsamettin,GÜN GÖK Zehra,arat esra,Ekici Seda,YİĞİTOĞLU Mustafa,Vargel ibrahim Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. Ankara Üniversitesi Veteriner Fakültesi Dergisi 70, no.1 (2023): 87 - 96. 10.33988/auvfd.1014802
MLA	BOZKAYA OGÜN,Ekici Hüsamettin,GÜN GÖK Zehra,arat esra,Ekici Seda,YİĞİTOĞLU Mustafa,Vargel ibrahim Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.70, no.1, 2023, ss.87 - 96. 10.33988/auvfd.1014802
AMA	BOZKAYA O,Ekici H,GÜN GÖK Z,arat e,Ekici S,YİĞİTOĞLU M,Vargel i Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2023; 70(1): 87 - 96. 10.33988/auvfd.1014802
Vancouver	BOZKAYA O,Ekici H,GÜN GÖK Z,arat e,Ekici S,YİĞİTOĞLU M,Vargel i Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2023; 70(1): 87 - 96. 10.33988/auvfd.1014802
IEEE	BOZKAYA O,Ekici H,GÜN GÖK Z,arat e,Ekici S,YİĞİTOĞLU M,Vargel i "Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 70, ss.87 - 96, 2023. 10.33988/auvfd.1014802
ISNAD	BOZKAYA, OGÜN vd. "Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract". Ankara Üniversitesi Veteriner Fakültesi Dergisi 70/1 (2023), 87-96. https://doi.org/10.33988/auvfd.1014802