Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation
Yıl: 2022 Cilt: 18 Sayı: 4 Sayfa Aralığı: 451 - 460 Metin Dili: İngilizce DOI: 10.22392/actaquatr.1074431 İndeks Tarihi: 18-01-2023
Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation
Öz: In this research, it was aimed to investigate the production of water-soluble chitosan from the enzymatic deacetylation of chitin by Bacillus (B. cereus (BC) and B. thuringiensis (BT)) strains. Characteristic properties involving molecular weight, degree of deacetylation, and antibacterial activity of chitosan samples were identified. The degree of deacetylation of BC and BT chitosan samples obtained at 393 and 213 ppm amounts was reported as 80.54% and 86.07% by the IR spectrum. As well as the degree of deacetylation, the molecular weights of samples showed a significant effect on antibacterial activity were 48.27 and 48.46 Da, respectively. Among the tested bacteria, the highest inhibitory effect was recorded in Pseudomonas aeruginosa and Staphylococcus epidermis for both chitosan samples. On the other hand, no antibacterial effect on S. aureus, Vancomycin-resistant Enterococci, S. epidermidis, Klebsiella sp., and Salmonella spp. were observed. Our results indicated a simple and cost-efficient method for the production of chitosan polymers showing antibacterial activity.
Anahtar Kelime: Enzimatik Deasetilasyon ile Üretilen Su Çözülebilir Kitosan Bileşiklerinin Antibakteriyel Aktivitesi ve Karakterizasyonu
Öz: Bu araştırmada, Bacillus sp. (B. cereus (BC) ve B. thuringiensis (BT)) suşları ile kitinin enzimatik deasetilasyonundan suda çözülebilir kitosan üretiminin incelenmesi amaçlanmıştır. Kitosan örneklerinin moleküler ağırlıkları, deasetilasyon dereceleri ve antibakteriyel aktivitelerini içeren karakteristik özellikleri tanımlanmıştır. 393 (BC) ve 213 (BT) ppm miktarda elde edilen kitosan örneklerinin deasetilasyon derecesi IR spektrumu ile sırasıyla %80,54 ve %86,07 olarak belirlenmiştir. Deasetilasyon derecesinin yanı sıra antibakteriyel aktivitede önemli bir etki gösteren moleküler ağırlık değerleri ise 48,27 ve 48,46 Da’dır. Her iki kitosan bileşikleri için, test bakterileri arasında en yüksek inhibitör etki P. aeruginosa ve S. epidermis’e karşı kaydedilmiştir. Bunun yanı sıra S. aureus, Vankomisin dirençli Enterococci, S. epidermidis, Klebsiella sp. ve Salmonella spp’ye karşı hiçbir antibakteriyel etki gözlenmemiştir. Bizim sonuçlarımız, antibakteriyel aktivite gösteren kitosan polimerlerin üretimi için basit ve düşük maliyetli bir yönteme işaret etmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | mercimek h, MATYAR F, yılmaz f, GUZELDAG G, çelik i (2022). Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. , 451 - 460. 10.22392/actaquatr.1074431 |
| Chicago | mercimek hatice aysun,MATYAR FATIH,yılmaz fatma,GUZELDAG GULCIHAN,çelik ibrahim halil Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. (2022): 451 - 460. 10.22392/actaquatr.1074431 |
| MLA | mercimek hatice aysun,MATYAR FATIH,yılmaz fatma,GUZELDAG GULCIHAN,çelik ibrahim halil Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. , 2022, ss.451 - 460. 10.22392/actaquatr.1074431 |
| AMA | mercimek h,MATYAR F,yılmaz f,GUZELDAG G,çelik i Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. . 2022; 451 - 460. 10.22392/actaquatr.1074431 |
| Vancouver | mercimek h,MATYAR F,yılmaz f,GUZELDAG G,çelik i Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. . 2022; 451 - 460. 10.22392/actaquatr.1074431 |
| IEEE | mercimek h,MATYAR F,yılmaz f,GUZELDAG G,çelik i "Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation." , ss.451 - 460, 2022. 10.22392/actaquatr.1074431 |
| ISNAD | mercimek, hatice aysun vd. "Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation". (2022), 451-460. https://doi.org/10.22392/actaquatr.1074431 |
| APA | mercimek h, MATYAR F, yılmaz f, GUZELDAG G, çelik i (2022). Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. Acta Aquatica Turcica, 18(4), 451 - 460. 10.22392/actaquatr.1074431 |
| Chicago | mercimek hatice aysun,MATYAR FATIH,yılmaz fatma,GUZELDAG GULCIHAN,çelik ibrahim halil Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. Acta Aquatica Turcica 18, no.4 (2022): 451 - 460. 10.22392/actaquatr.1074431 |
| MLA | mercimek hatice aysun,MATYAR FATIH,yılmaz fatma,GUZELDAG GULCIHAN,çelik ibrahim halil Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. Acta Aquatica Turcica, vol.18, no.4, 2022, ss.451 - 460. 10.22392/actaquatr.1074431 |
| AMA | mercimek h,MATYAR F,yılmaz f,GUZELDAG G,çelik i Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. Acta Aquatica Turcica. 2022; 18(4): 451 - 460. 10.22392/actaquatr.1074431 |
| Vancouver | mercimek h,MATYAR F,yılmaz f,GUZELDAG G,çelik i Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation. Acta Aquatica Turcica. 2022; 18(4): 451 - 460. 10.22392/actaquatr.1074431 |
| IEEE | mercimek h,MATYAR F,yılmaz f,GUZELDAG G,çelik i "Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation." Acta Aquatica Turcica, 18, ss.451 - 460, 2022. 10.22392/actaquatr.1074431 |
| ISNAD | mercimek, hatice aysun vd. "Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation". Acta Aquatica Turcica 18/4 (2022), 451-460. https://doi.org/10.22392/actaquatr.1074431 |
