Objective: This study was conducted to determine the relationship between pregnant women's health literacy level and health practices. Method: The research is descriptive. The sample of the study consisted of 250 pregnant women who applied to the outpatient clinic of a Maternity Hospital in June 2019 for antenatal care. In the collection of the data, the Personal Information Form, Health Practice Questionnaire in pregnancy, Turkey Health Literacy Survey Scale-32 (TSOY-32) was used. Percentage distribution, mean, standard deviation, Pearson Correlation Analysis and one-way Anova test were used to evaluate the data. Results: The mean age of the pregnant women was 28.0±6.0 year and the mean pregnancy week was 34.4±4.3. The mean total health literacy score of pregnant women is 36.1±9.7. It was determined that 12.8% of the pregnant women were inadequate, 24.0% had problematic limited, 28.4% were adequative, and 34.8% had excellent health literacy. The mean score of health practices of pregnant women was 97.5±11.6. It was determined that there was a positive statistically significant relationship between general health literacy and all sub-literacy indexes and the mean health practices of pregnant women (p<0.01). Conclusion: Although the health literacy level of the pregnant was found to be adequative in general, the health literacy of a significant part of the pregnant women was found to be inadequate and problematic limited level. As the health literacy of pregnant women increases, health practices increase.